Insect-derived promoters for foreign proteins expression in insect cells

A protein and promoter technology, applied in the biological field
CN102203264AInactive Publication Date: 2011-09-28替代基因表达有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
替代基因表达有限公司
Publication Date
2011-09-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

Insect-derived promoters for foreign proteins expression in insect cells. Regulatory polynucleotide sequences which drive the expression of major insect proteins (hexamerins) at specific evolution stages of larva have been isolated from insects (Trichoplusia ni) in the present invention. Said regulatory polynucleotide sequences promote stronger foreign gene expression in the baculovirus system than the conventional polyhedrin promoter. Additionally, the combination of the new larva-derived promoters of the invention with the pL promoter increased baculovirus expression levels up to 61%-375% (depending on the time of infection) with respect to conventional baculo viruses used in the biotechnology industry. Promoter pB2 also drives gene expression at earlier times than polyhedrin promoter, being this feature a great advantage for correct protein folding and posttranslational modification.
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Description

technical field

[0001] The invention may relate to the field of biotechnology. The present invention relates to regulatory polynucleotide sequences (such as promoters) derived from hexamerin family genes in insects, and their use for expression of foreign proteins in insect cells and insect larvae, for example using baculovirus expression vectors. Background technique

[0002] The baculovirus-insect cell expression system is versatile and widely used to produce heterologous (native and recombinant) proteins. This system is generally accepted as one of the most powerful and versatile systems to produce recombinant proteins of any size. It is based on two main properties: high levels of protein expression and proper protein folding to become biologically active, along with important post-translational modifications in mammalian cells. In addition, the development time of this system is short, and the genetic stability of the recombinant virus is very high. The baculovirus g...

Claims

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