2 results about "Molting Hormone" patented technology

This utility model belongs to the field of forestry pest control technology, and particularly relates to a longhorn beetle trapping and killing device, including a column and a base. An observation mechanism is located in the middle of the outer surface of the column, and a trapping box is connected to the bottom of the observation mechanism. The outer surface of the trapping box is fixedly connected to two trapping entrances, with heights of 100cm and 50cm respectively. Trapping box one contains a bark crevic filled with a pheromone lure from the red-edged longhorn beetle. Trapping box two contains a metabolite mimicry agent of the sea buckthorn wood-boring moth larvae, specifically an artificially synthesized odor of its feces or molting hormone. This longhorn beetle trapping and killing device, with two different trapping entrances, can cover active groups at different times and heights, specifically capturing upward-migrating larvae and adults. It also accommodates the spatial competition characteristics of the two pests, expanding the trapping range and enhancing the trapping effect through their competitive relationship. By confusing the odors, it attracts more red-edged longhorn beetle adults, improving trapping efficiency.

This invention relates to the field of biological control of agricultural pests, and particularly to a VIGS recombinant vector targeting the CYP314A1 gene of the tomato leafminer and its insecticidal application. This invention clones the CYP314A1 gene of the tomato leafminer, which is highly expressed in the epidermis of 2nd-3rd instar larvae and is a key gene for molting hormone synthesis. This invention constructs a pTRV2-TaCYP314A1 recombinant vector, combined with a pTRV1 helper vector. After infecting tomato plants with Agrobacterium GV3101, it induces the continuous endogenous synthesis of dsTaCYP314A1 in the plant. After feeding, the tomato leafminer triggers RNA interference, causing molting deformities, pupation, and death in the larvae; the larval mortality rate reaches over 80% after 7 days. This invention is a non-GMO technology, the product is easily degradable, and it is safe for non-target organisms such as tobacco mirid bugs, earthworms, and parasitic wasps. This invention solves the problems of easy degradation of free dsRNA, short duration of effect, and poor efficacy of traditional targets, and can achieve long-term insect resistance in Solanaceae crops throughout the entire growth period, with broad prospects for industrial application.