39 results about "Molting Hormone" patented technology

The scorpion breeding method in nature simulating condition includes providing cultivation pond with different temperature and humidity conditions for scorpion to select suitable inhabiting environment and adopting animal and plant complementing mode; providing composite feed including yellow meal worm, ground beetle, artificially compounded feed and molting hormone; and adopting loam and efflorescent wall soil as breeding soil. The present invention can raise the scorpion yield greatly.

The invention discloses feed capable of promoting molt of crayfish and a preparation method thereof and belongs to the field of agricultural feed. The feed is prepared by mixing rice bran powder, soybean meal, fish meal, seaweed powder, bone meal, squid liver paste, cholesterol powder, tricalcium phosphate, calcium hydrogen phosphate, vitamin D3, tea seed meal, potassium dihydrogen phosphate, bile acid and aquatic complex microbial agent and then performing fermentation. The feed has the advantages that the molting hormone of the crayfish is increased by promoting the crayfish to absorb the high cholesterol in the feed to synthesize ecdysterone so as to increase the molt and growth speed of the crayfish; the feed can promote the molt of the crayfish, increase the immunity of the crayfish, increase the number of intestinal tract beneficial bacteria and purify water; drugs such as hormone and antibiotics are not added into the feed, and the feed is simple to prepare, safe in raw materials, rich in nutrition, scientific in compatibility, good in feeding attraction performance, low in cost and capable of increasing benefits.

The invention discloses feed capable of promoting the molt of Penaeus vannamei and a preparation method thereof and belongs to the field of agricultural feed. The feed is prepared by mixing rice bran, bran, bean cake, minced fish meat, seaweed meal, squid liver paste, shrimp shell meal, salted duck egg, bile acid, radix astragali, Eucommia ulmoides, Adenophora stricta, radix rehmanniae preparata, dendrobium, fructus schizandrae, ginseng leaf, trace element agent and microbial fermentation agent and then performing fermentation. The feed has the advantages that the molting hormone of the Penaeus vannamei is increased by promoting the Penaeus vannamei to absorb the high cholesterol in the feed to synthesize ecdysterone so as to increase the molt and growth speed of the Penaeus vannamei; the feed can promote the molt of the Penaeus vannamei, increase the immunity of the Penaeus vannamei, increase the number of intestinal tract beneficial bacteria and purify water; drugs such as hormone and antibiotics are not added into the feed, and the feed is simple to prepare, safe in component, rich in nutrition, scientific in compatibility, good in feeding attraction performance and low in cost.

The invention relates to a feed additive for promoting growth of bred shrimps and crabs after shelling. The feed additive is prepared by screening the following components with an 80-mesh sieve and uniformly mixing the components in percentage by weight: 2 to 5 percent of beta-molting hormone, 3 to 15 percent of antibacterial peptide, 5 to 10 percent of yeast, 10 to 20 percent of bacillus subtilis and the balance of maize starch, wherein the molecular structure of the molting hormone is C27H44O7 and high performance liquid chromatography (HPLC) is more than or equal to 85 percent; the total number of viable bacteria in every gram of yeast is more than or equal to 50 hundred millions; and the total number of viable bacteria in every gram of bacillus subtilis is more than or equal to 200 hundred millions. The feed additive is preferably prepared by screening the following components with the 80-mesh sieve and uniformly mixing the components in percentage by weight: 5 percent of beta-molting hormone, 12 percent of antibacterial peptide, 8 percent of yeast, 15 percent of bacillus subtilis and 60 percent of maize starch, wherein the molecular structure of the molting hormone is C27H44O7 and high performance liquid chromatography (HPLC) is more than or equal to 85 percent; the total number of viable bacteria in every gram of yeast is more than or equal to 50 hundred millions; and the total number of viable bacteria in every gram of bacillus subtilis is more than or equal to 200 hundred millions. The feed additive has a rational formula, the preparation method is simple, and metabolism and in-vivo protein synthesis of the bred shrimps and crabs are promoted through the synergistic effect of the components, so that the autoimmunity and the digestion functions of the shrimps and the crabs can be improved while the shrimps and the crabs are shelled, the growth is promoted, the survival rate is increased and the economic benefit is obviously improved.

The invention discloses a growth promoting premix compound for shrimps and crabs. The growth promoting premix compound comprises the following components in percentage by weight: 0.01-0.05 percent of L-carnitine raw material, 1-2 percent of lecithin raw material, 2-4 percent of inositol raw material, 4-5 percent of glycine raw material, 3.5-5 percent of lysine raw material, 11-15 percent of potassium chloride raw material, 0.5-0.9 percent of molting hormone raw material, 1-8 percent of vitamin C raw material, 1-5 percent of astragalus polysaccharides raw material and 58-73 percent of glucose raw material. A preparation method of the growth promoting premix compound for shrimps and crabs comprises the following steps of: weighing the raw materials according to the weight ratio; adding intoa mixer for mixing for 5-10 minutes till the uniformity is up to 5 percent to obtain a mixture; and packaging. By adopting the premix compound, the stress resistance of shrimps and crabs can be improved, the feed utilization ratio is increased, and the dehulling and growth of shrimps and crabs are facilitated simultaneously.

The invention provides a method for producing plate cocoons and white muscardine silkworms simultaneously. The method mainly comprises the following steps: after silkworms are raised to fifth-instar silkworms, according to fungus strain implantation nodes, beauveria spores are implanted into silkworm bodies, when matured silkworms appear, mulberry leaves which are sprayed with a molting hormone are applied to feed the silkworms, when the silkworms spin and begin to have a little urination, the silkworms are laid on a spinning bed, the angle of the spinning bed is adjusted to make the silkworms crawl uniformly and spin, when silkworms close feet and fall, all silkworms are swept from the spinning bed, the swept silkworms are put into a bamboo basket uniformly, the plate cocoons are collected, the bamboo basket is put into a white muscardine silkworm producing room which has a temperature and humidity until muscardine silkworms are white, baking or drying in the sun is performed, and therefore the white muscardine silkworm finished products are obtained. According to the method, production of the plate cocoons and production of the white muscardine silkworms are combined, so that the yield of the plate cocoons can be guaranteed, economic benefits of producers are improved, and meanwhile the rigidification rate of the produced white muscardine silkworms is high, the cleanness degree of stiff silkworm is high, and drug efficacy meets requirements of Chinese pharmacopoeia.

The extraction method of insect molting hormone analog in bulge relates to extraction of botanical insect hormone analog. The extraction method of insect molting hormone analog in bulge is one combining alcohol extraction, water precipitation and ultrasonic extraction. Through ultrasonic vibration and repeated extraction of the residue, the present invention has high extracting rate, high extract purity, simple extraction process and no environmental pollution.

The invention provides an ajuga multiflora bunge regeneration system and a tissue culture method of a rapid propagation method, belonging to the technical field of in-vitro propagation of plant seedling and laying a good foundation for a cell or tissue culture technology which takes plant molting hormone analogues as target products. The method comprises the following steps of: respectively taking a culture medium as a raw material and doping 1mg of 6-BA and 0.2mg of NAA to prepare a mud-induction culture medium, wherein the culture medium is an MS culture medium and the pH value is 5.8 to 6.5; doping 1mg of 6-BA and 0 to 1mg of 2,4-D to prepare an induced healing culture medium; doping 0 to 2mg of 6-BA to prepare an auxiliary-bud enrichment culture medium; doping 0 to 1mg of IBA (NAA) to prepare a rooting culture medium; selecting leaves and carrying out induced culture after asepsis, wherein the leaves can germinate and grow strong new buds after 30 to 35 days; then carrying out enrichment culture for 25 to 30 days, carrying out rooting culture for 25 to 30 days to form seedlings, and transplanting, wherein the survival ratio reaches more than 95 percent. The ajuga multiflora bunge tissue culture and rapid propagation method aims at the characteristics of ajuga multiflora bunge, is simple, convenient and easy to practice and free of deformity of tissue culture seedlings and has improved propagation coefficient, short propagation period, good culturing effect and high survival ratio.

A process for increasing the expression to exogenous gene in the insect baculovirus system by insect hormones features that the molting hormone (MH), the juvenile hormone (JH) and their mixture are used to increase the expression efficiency of exogenous gene and polyhedron protein gene in the insect baculovirus system and the copying efficiency of insect baculovirus in insect cells.

The invention provides a functional fresh water crayfish feed and a preparation method and a use method thereof. The functional fresh water crayfish feed is prepared from the following raw material components in parts by weight: 10-20 parts of bean meal, 10-15 parts of sunflower kernel meal, 13-20 parts of Canada rapeseed meal, 5-15 parts of peanut meal, 15-20 parts of high gluten flour, 10-20 parts of fish meal, 3-5 parts of shrimp shell powder, 2-3 parts of squid paste, 3-5 parts of pine needle powder, 3-5 parts of spirulina powder, 1-2 parts of a yeast extract, 1-2 parts of phospholipid oil, 2-3 parts of fish oil, 1-2 parts of monocalcium phosphate, 0.5-1 part of a Chinese herbal medicine additive, 0.1-0.2 part of choline, 0.05-0.15 part of a molting hormone, 0.5-1.0 part of complex vitamin, and 0.5-1.0 part of complex mineral. The functional freshwater crayfish feed has more comprehensive and balanced nutrition, and crayfishes grow fast, and have better meat sense and taste.

The invention provides a compound preparation for promoting the shedding and growth of shrimps and crabs and a preparation method thereof. The compound preparation consists of 0.5 to 0.9 weight part of molting hormone, 40 to 60 weight parts of oriental waterplantain rhizome, 65 to 85 weight parts of gentian, 70 to 80 weight parts of common selfheal fruit-spike, 20 to 30 weight parts of glucan and 20 to 30 weight parts of zeolite. The preparation method comprises: weighing the molting hormone, the oriental waterplantain rhizome, the gentian, the common selfheal fruit-spike, the glucan and the zeolite, which serve as raw materials, in weight part; and uniformly stirring the raw materials to obtain the compound preparation. Through the synergy of the component, the compound preparation for promoting the shedding and growth of shrimps and crabs can promote the metabolism and in-vivo protein synthesis of shrimps and crabs to allow shrimps and crabs to shed in time and can improve the immunity and digestion of shrimps and crabs, promote growth and improve survival rate; and the preparation method is simple and meets the needs for large-scale industrial production.

The invention relates to a technology for extracting beta-molting hormone from tussah in a solvent-assisted high-voltage pulsed electric field. The technology is characterized by comprising the following steps of: (1) pretreatment of raw materials; (2) crude extraction; (3) extraction in the high-voltage pulsed electric field: adding 30% of acetic acid solution according to a ratio of feed to liquid being 1:(18-22), placing the mixed liquid in the high-voltage pulsed electric field, and extracting for 1-2 hours at 35-45 DEG C, the electric field intensity of 8-10kv.cm<-1> and pulse number of 8-12 to obtain an extracting solution; and (4) purification: filtering the extracting solution under reduced pressure, and separating and extracting with macroporous resin to obtain the beta-molting hormone. The technology for extracting the beta-molting hormone from the tussah in the solvent-assisted high-voltage pulsed electric field is low in extraction temperature and short in extraction time, ensures the stability of beta-molting hormone extraction and has the advantages of time saving, high efficiency and stability; and simultaneously the yield of the beta-molting hormone is obviously improved.

The invention discloses a molting hormone extracting method. According to the method, methyl alcohol or a methyl alcohol-acetone mixed solution is used to soak cyanotis arachnoidea to extract moltinghormone, and then the molting hormone is obtained through decoloring, crystalizing and recrystallizing. The method is simple and easy to operate, does not need column chromatography, is low in cost and short in period, and is suitable for industrial application and market promotion.

The invention provides a molting hormone regulatory factor E78-C gene cDNA of cotton bollworm. A molting hormone induction factor E78-C gene is cloned from the cotton bollworm; a primer is designed for the molting hormone induction factor E78-C gene of the cotton bollworm; the 658th to the 1122nd in the SEQ ID No. 1 are used as an E78-C target gene fragment to synthesize dsRNA, and then the synthesized dsRNA is transferred to the body cavity of the cotton bollworm by microinjection; a green fluorescent protein (GFP) is used as a control group; and the counting of the egg laying amount of the cotton bollworm injected with dsRNA indicates that when the E78-C gene of the cotton bollworm is used as an interference target gene to carry out injection interference on the cotton bollworm and transcriptional expression of a silent scene, the ovary development of the cotton bollworm is inhibited, the egg laying amount is reduced, and further the population quantity of the cotton bollworm is controlled. The risk that injurious pests have drug resistance is low. A theoretical basis is provided for the creation of novel biological insecticides and plant pest-resistant genetic engineering.

The present invention discloses an anti-stress compound preparation. The anti-stress compound preparation comprises the following components in parts by mass: 20-25 parts of glycine, 10-15 parts of methylenetetrahydrofolic acid, 10-15 parts of calcium lactate, 2-5 parts of vitamins, 1-3 parts of molting hormones, 1-3 parts of potassium ferrate, 5-9 parts of polyaluminum chloride and 1-3 parts of sodium hypochlorite. The present invention also discloses a preparation method of the anti-stress compound preparation. The compound preparation is polymerized to form glucose in aquaculture water bodyand can remain in the aquaculture water for a long time; the excessive glycine can be directly ingested by shrimps to improve the immunity of the shrimps; the calcium lactate ensures supplement of calcium in the shelling process of the shrimps; besides, the molting hormones promote the concentration shelling of the shrimps in the same aquaculture water body, avoid the occurrence of death after the shelled shrimps are attacked by the same kind for food; the compound preparation disinfects the water body, avoids produced lesions and stress due to excessive pathogenic bacteria during the shelling of the shrimps; and the potassium ferrate can effectively remove algae substances in the aquaculture water body and increase the oxygen content in the aquaculture water body.

The invention discloses a method for breeding and cultivating cyanotis arachnoidea. The method mainly comprises the steps of seed selection and harvesting, seedbed preparation, sowing, seedbed management, field preparation, transplantation, tillage management, harvesting and the like. According to the method, biological characteristics are utilized, control measures for each key planting link are detailed on the basis of a conventional cyanotis arachnoidea planting technology, the fertilization ratio and the planting density are optimized, and a rare earth fertilizer and a biological organic fertilizer are applied according to a reasonable proportion after seedling raising and transplantation, so that the growth of the cyanotis arachnoidea can be effectively promoted, the proportion of nutritional ingredients in plants is optimized, the effects of improving the yield of a unit area and increasing the beta-molting hormone content are achieved under the conditions of reducing the fertilizing amount and expanding a planting row pitch, the planting cost is lowered, the planting return rate is increased, the income increase and the production increase of a farmer are promoted, the development of the cyanotis arachnoidea industry can be effectively promoted, and the method has remarkable economic benefits and social benefits.

The invention relates to a feed for preventing red speck disease of freshwater shrimps. The feed contains the following components: imported fish meal, soybean pulp, cottonseed meal, rapeseed cakes, rapeseed meal, DDGS, alpha starch, phosphorous calcium, choline, adhesive, premix, molt hormone, salt, rapeseed oil and a feed additive. By improving the proportion of the materials, the feed is rich in nutrition, good in palatability and high in utilization rate. The feed for preventing red speck disease of freshwater shrimps contains feed additive component, thus having the efficacies of resisting insects and sterilizing, clearing heat and detoxifying and the like. In the early period of cultivation, the freshwater shrimps are fed with the feed for preventing the red speck disease of the freshwater shrimps for one week, the red speck disease can be effectively prevented, the morbidity of the freshwater shrimps can be reduced, and the cultivation benefits can be guaranteed.

A material and a system are provided that efficiently inactivate a molting hormone by means of protein (a) or (b) below, that is, (a) a protein having an amino acid sequence shown by Sequence Listing SEQ ID NO:2, or (b) a protein having an amino acid sequence in which one or a plurality of amino acids are deleted, substituted, or added in Sequence Listing SEQ ID NO:2, the protein having ecdysteroid 22-oxidase activity.