The invention relates to a method for preserving tissue culture bulbs of fritillary viruses, which belongs to the technical field of plant virus preservation and comprises following steps: identifying pathogeny species of the viruses, preparing culture medium, culturing virus-free tissue culture bulbs, testing virus ELISA, vaccinating virus by friction, and preserving virus bulbs. The invention has the advantages that the tissue culture bulbs of the fritillary viruses are used to infect the purified viruses, and then the viruses of fritillary tissue culture bulbs are preserved, which overcomes the defects in traditional preservation, can prevent viruses from confounding and losing, is easy to extract and prepare infectious titer, provides virus with high purity for preparing antiviral breeding and antiviral serum, lowers preserving cost, increases preserving effects, and is easy to carry and communicate.