Method for extracting cordycepin

An extraction method and technology for cordycepin, which are applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve problems such as microwave/light wave reports that have not yet been seen, and achieve long extraction time, low extraction rate, and infection. strong effect

Active Publication Date: 2013-02-20
SHIJIAZHUANG ZANGNUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] So far, there has been no report on the extraction of cordycepin by microwave / light wave

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0056] Preparation of cordycepin fermented liquid:

[0057] The cordycepin fermented liquid can be purchased commercially. In order to ensure the controllability of its raw materials, the present invention adopts the following method to prepare:

[0058] The fermentation medium is a liquid medium, and the weight percentage of each component in the medium is: sucrose 4.0%, corn juice 3.0%, bran 3.0%, whole milk powder 1.0%, VB 0.01%, KH 2 P0 4 1%, MgS0 4 0.05%.

[0059] The specific fermentation method is as follows: add medium into the fermenter, insert bacteria after sterilization, inoculum amount is 10%, and the fermentation conditions are: 22°C, 150r / min, pH6-7, so that the bacteria grow in suspension in the liquid, The culture time is 6 days, and the content of cordycepin reaches above 0.5%, and the cordycepin fermentation liquid is obtained.

[0060] The quality control index of the content of cordycepin in the fermentation broth is determined by liquid chromatography...

Embodiment 1

[0062] Embodiment 1: the extraction method of cordycepin

[0063] 1. Use a rotary evaporator to concentrate the cordycepin fermented liquid to 1 / 3 times the volume of the original liquid, extract it in a microwave oven with a combination of 55% microwave and 45% light wave, and a radiation power of 800W for 8 minutes, and centrifuge the extract. Centrifuge at 5000 rpm for 10 minutes, and collect the supernatant;

[0064] 2. Put the supernatant obtained in step 1 on a rotary evaporator, concentrate under reduced pressure to 1 / 5 of the original volume, and then use 3 times the volume of the concentrated solution to precipitate with absolute ethanol, overnight, then collect the supernatant, and use 5000r / min Centrifuge for 15 minutes, collect the supernatant, then mix with an equal amount of activated carbon, shake on a shaker for 12 hours, filter to remove the pigment, and obtain the cordycepin supernatant;

[0065] 3. Pass the cordycepin supernatant obtained in step 2 through ...

Embodiment 2

[0070] Embodiment 2: the extraction method of cordycepin

[0071] 1. Depressurize the cordycepin fermentation liquid according to the concentration ratio to 1 / 5 times of the original liquid. Ultrasonically extract it in a microwave oven with 55% microwave and 45% light wave combination and a radiation power of 800W for 5 minutes, and centrifuge the extract at a speed of 5000r / min And the time is 10min, collect the supernatant;

[0072] 2. Put the supernatant obtained in step 1 on a rotary evaporator, concentrate under reduced pressure to 1 / 5 of the original volume, and then use 3 times the volume of the concentrated solution to precipitate with absolute ethanol, overnight, then collect the supernatant, and remove the supernatant The solution was centrifuged at 5000r / min for 15min, then mixed with an equal amount of activated carbon, shaken on a shaker for 12h, filtered to remove the pigment, and the supernatant of cordycepin was obtained;

[0073] 3. Pass the cordycepin super...

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PUM

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Abstract

The invention relates to a method for extracting cordycepin. The method includes the following steps of subjecting a cordycepin fermentation liquid to reduced pressure concentration, then performing ultrasonic extraction in combinations of microwaves and light waves respectively, and further subjecting an obtained extraction liquid to separation and purification to obtain the cordycepin. Compared with traditional extraction methods, the method has the advantages that by means of the method, the extraction ratio is improved, extraction time is saved, and purity is increased.

Description

technical field [0001] The invention relates to an extraction method of active ingredients, in particular to an extraction method of cordycepin. Background technique [0002] Cordycepin (cordycepin) is also known as 3'-deoxyadenosine, Cordyceps, Cordyceps militaris, Cordyceps cephalosporin mycelium, 3-deoxyacyl glycosides, Cordyceps sinensis, Cordyceps sinensis. [0003] Cordycepin is the main active ingredient in Cordyceps militaris (especially nucleosides), and it is also the first nucleoside antibiotic isolated from fungi. [0004] Cordycepin (Cordycepin) is an analogue of adenosine, molecular formula: C 10 h 13 N 5 o 3 , Molecular weight: 251.24, basic, needle-like or flaky crystal, melting point 230°C-231°C, maximum absorption wavelength 259.0nm. [0005] In 1951, Cunningham in Germany isolated and discovered cordycepin from the culture filtrate of Cordyceps militaris. [0006] Since the 1960s, the synthesis and extraction of cordycepin has attracted widespread at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/16C07H1/08
Inventor 王智森高飞
Owner SHIJIAZHUANG ZANGNUO BIOTECH
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