Method for separating halimasch from endophytic fungi of gastrodia elata
A technology of endophytic fungi and armillaria, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of strain degradation, difficult operation, easy contamination of miscellaneous bacteria, etc., and achieve the goal of reducing mold Pollution, avoidance of miscellaneous bacteria contamination, less stimulation by drugs
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Embodiment 1
[0031] Embodiment one: from the experiment of armillaria mellea from the endophytic fungus of arrowroot
[0032] 1 Material:
[0033] Arrow hemp is collected from the main growth point of Gastrodia elata in Qinba mountainous area.
[0034] Improved PDA medium: potato (peeled) 200.0g, corn flour 30.0g, glucose 20.0g, agar 12.0g, KH2PO4 5.0g, MgSO4 3.0g, peptone 5.0g, VB1 10.0mg, pH natural, add water to 1000mL ; Dispense into test tubes, the liquid volume is 1 / 3 of the test tubes, put them upright after autoclaving, and wait for use.
[0035] Antibiotic medium: add chloramphenicol (25 μg / mL) and streptomycin (10 μg / mL) to PDA medium, after the medium is sterilized by high temperature and high pressure, cool to about 40-50°C and add chloramphenicol and streptomycin . Wherein the stock solution concentration of chloramphenicol is 34 mg / mL, and the stock solution concentration of streptomycin is 10 mg / mL.
[0036] 2 methods
[0037] 2.1 Arrow gastrodia pretreatment: select the ...
Embodiment 2
[0054] Embodiment two Gastrodia elata and armillaria cultivation test
[0055] 1 material
[0056] A total of 18 Armillaria strains from ZY-1 to ZY-18 were selected for the test Armillaria, and the germinating bacteria were Dendrobium small mushrooms. Gastrodia elata capsules used in the experiment: including Gastrodia elata red and Gastrodia green stalks, all collected from the natural distribution area of Gastrodia elata in Qinba Mountains. Cultivation fungus material: the fungus material used for cultivation selects the green gang wood material, large wood (length 30cm, diameter 8cm), small wood (length 10cm, diameter 2cm), section wood (length 3cm, diameter 2cm). Cut the fish scales with a depth of about 2cm before using large and small materials.
[0057] 2 methods
[0058] 2.1 Strain preparation: Armillaria tertiary species preparation: select pre-separated and screened Armillaria strains to prepare Armillaria cultivars. The cultivars use 8×17cm plastic cultivation ...
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