42 results about "Ecdysteroid" patented technology

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-cyano-2-methyl-6[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, an N-oxide, or a salt thereof, Formula (1) and (b) at least one invertebrate pest control agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and salts of the foregoing. Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-cyano-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, an N-oxide, or a salt thereof, Formula (1) and (b) at least one invertebrate pest control agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and salts of the foregoing. Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

The invention relates to water splashing compound preparation for boosting exuviation of shrimps and crabs, hardening shells, preventing diseases and resisting stresses and belongs to the technical field of aquiculture. Glucose, sodium chloride, monopotassium phosphate, magnesium sulfate, calcium chloride, calcium lactate, taurine, trimethyl glycine, vitamin C, ecdysone and Chinese herbal medicament are chosen as the compound components of the water splashing compound preparation. The compound components are weighted in proportion, and then are mixed uniformly and packaged in batch, and finally are packaged and placed in a warehouse. Various nutrient components which are safe and nontoxic to shrimps and crabs are adopted by the invention; the functions of boosting exuviation, supplementing calcium and hardening shells, preventing diseases and eliminating stresses, and the like, are integrated; the components are clear; the effect is stable; the water splashing compound preparation can be directly splashed and conveniently used; the bioavailability is high and is not influenced by environmental condition; and after the water splashing compound preparation is used, the normal physiological functions and immunologic functions of cultivated shrimps and crabs are quickly recovered, thereby achieving the functions of boosting exuviation, supplementing calcium and hardening shells, preventing diseases and eliminating stresses, and increasing the survival rate and growth rate of the cultivated shrimps and crabs.

The invention discloses a method for extracting ecdysone from common cyanotis roots and the ecdysone thereof, belonging to the technical field of the extraction and the separation of effective components of plants. The method comprises the following steps of: decocting 0.5-1kg of common cyanotis roots by 10L of water, or extracting by using 1-95 percent methanol or ethanol, and the ratio of the using amount of the methanol or the ethanol to the common cyanotis roots is 5L-10L to 1kg; filtering, decompressing and concentrating to obtain liquid with the relative density of 1.05-1.20; regulating a pH value to 6-10 by using alkali; then extracting by a mixed solvent with normal butanol and ethyl acetate, wherein the ratio of the normal butanol and the ethyl acetate is 4:6-0.5:9.5; washing, decompressing and concentrating to obtain a fluid extract with the relative density of 1.2-1.3; adding 50-95 percent methanol or ethanol for dissolving and dilution, passig through overhigh alkali type anion exchange resin, and eluting, concentrating and drying in a decompressing way to obtain the ecdysone. Compared with a conventional method, the method enhances the yield by 1.16-1.38 percent.

Disclosed are mixtures and compositions for controlling invertebrate pests relating to combinations comprising (a) 3-bromo-N-[4-chloro-2-methyl-6-[(methylamino)carbonyl]phenyl]-1-(3-chloro-2-pyridinyl)-1H-pyrazole-5-carboxamide, and its N-oxides, and suitable salts thereofanda component (b) wherein the component (b) is at least one compound or agent selected from neonicotinoids, cholinesterase inhibitors, sodium channel modulators, chitin synthesis inhibitors, ecdysone agonists, lipid biosynthesis inhibitors, macrocyclic lactones, GABA-regulated chloride channel blockers, juvenile hormone mimics, ryanodine receptor ligands, octopamine receptor ligands, mitochondrial electron transport inhibitors, nereistoxin analogs, pyridalyl, flonicamid, pymetrozine, dieldrin, metaflumizone, biological agents, and suitable salts of the foregoing.Also disclosed are methods for controlling an invertebrate pest comprising contacting the invertebrate pest or its environment with a biologically effective amount of a mixture or composition of the invention.

The invention discloses cyanotis root extract rich in beta-ecdysone and a preparation method thereof. The cyanotis root extract comprises 50-90% of beta-ecdysone. The cyanotis root extract made from cyanotis root is prepared by water circulation extraction, centrifugal separation, resin absorption, and purification. The preparation method particularly includes: selecting annual or biennial cyanotis, removing impurities, taking dried root for grinding, adding 4-10 times of water for circulation extraction for 1.5-2.5 hours, performing centrifugal separation, applying macroporous resin absorption columns to liquor, eluting with ethanol, recycling elution crystallization, adding ethyl acetate for recrystalization, and drying to obtain cyanotis root extract. The cyanotis root extract made from annual or biennial cyanotis is rich in beta-ecdysone as raw material. The cyanotis is a biological product raw material having excellent properties, and is widely applicable to preparing and production of health-care products and pharmaceuticals. Beta-ecdysone content of the cyanotis root extract prepared by the method is increased greatly and can reach more than 90%. Biological activity of beta-ecdysone can be maintained effectively by low-temperature process control, and product quality is guaranteed.

The invention discloses a method for extracting ecdysone from spinacia oleracea. The method comprises the following steps: taking a to-be-detected sample; grinding liquid nitrogen into powder and adding a mixed solution of MeOH-CHCl3 in a volume ratio of 9:150ml or 100% MeOH solution until the solution is 50ml; vibrating at 37 DEG C for 2 hours and filtering by using silica gel powder; adding less NaCl and 50ml of n-Hexane to extract for two times; collecting an MeOH layer and drying by using a rotary evaporator; dissolving the sample again by using isopropanol; filtering and carrying out high performance liquid chromatography; determining the content. According to the method, an extract is subjected to the high performance liquid chromatography; the extraction speed is rapid and the extraction efficiency is high; the trace extraction of the extract can be realized.

The invention relates to a safe and high-efficiency pilatory and the preparation method thereof. The pilatory is made from 40 to 80 parts of cepharanthine, 5 to 15 parts of a red sage root extract, 10 to 30 parts of total saponin in notoginseng, 5 to 15 parts of a polygonum extract, 10 to 20 parts of a cochinchnese asparagus root extract, 5 to 15 parts of a prepared rehmannia root extract, 15 to 30 parts of a Chinese angelica root extract and 5 to 15 parts of ecdysone. Due to stronger permeability, the pilatory can be infiltrated to the root part of hair and stimulate the cell tissues of the root part of the hair to regenerate new hair by utilizing the specific medical speciality of the Chinese medicinal herbs. Meanwhile, the pilatory can improve the function of the scalp capillary, promote the blood circulation of the scalp, increase the nutrients needed by hair growth and quicken hair growth.

A biologically active food supplement intended to increase the athletes' muscle mass comprises Leuzea (or other medicinal plant containing ecdysteroids), male bee brood, ascorbic acid (Vitamin C). Vitamin E, and excipients (calcium stearate, talc, lactose) with the following ratio of components (mass %): Male bee brood 1-70; Leuzea 5-70; Ascorbic acid 1-70; Vitamin E 0.05-30; calcium stearate 0.92-0.95; talc 3-6; lactose—the rest.

The invention discloses a preparation method and an application of an ecdysone-rich plant standardized extract. The preparation method comprises the steps of: extracting ecdysone-rich plant materials using ethanol under reflux, filtering, concentrating and centrifuing; decolorizing the centrifuged supernatant, purifying by macroporous resin column chromatography, concentrating and drying to obtain the standardized extract. The method is simple and feasible, short in production period, and stable and controllable in quality, and is easy for preparation and industrial production in a standardized and batch manner; and the similarity of the extract obtained by the method is more than 99% by HPLC (high performance liquid chromatography) fingerprint spectrum detection. The extract can be applied in preparing fixed preparations serving as drugs for reducing blood sugar and resisting rheumatoid arthritis, can be used as a raw material for natural cosmetics and can be applied in animal breeding industry.

A genetically engineered virus of Chinese ballworm is obtained by double recombination of wild virus. Its genome is deficient is egt gene. At the position deficient in egt gene, the insect-specific neurotoxic AaIT gene controlled by the promoters of Chinese ballworm virus' alkaline DNA binding protein gene (p6.9) and polyhedrin protein gene (ph) and the beta-galactosidase (LacZ) gene controlled by the promoter of heat shock protein gene (hsp70) are inserted. Its advantages are higher insecticiding speed and better application effect.

The invention relates to a recombined insect molting hormone inactivation gene Bbsp::egt and an insecticidal fungistatic agent thereof. The recombined insect molting hormone inactivation gene Bbsp::egt contains coding beauveria bassiana chitinase signal peptide sequences (Bbsp) and coding lymantria dispar nuclepolyhedrovirus (hereinafter referred to as LdMNPV) ecdysteroid uridine 5minute-diphosphonic acid-glucosyltransferase gene (egt), and has a nucleotide sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.2. The molecular biological technique is adopted to shift into beauveria bassiana to obtain correct genetic transformation bacterial strains. The recombined Bbsp::egt bacterial strain obtained by the invention can disturb the normal operation of a hormone system in vivo of insects, and is beneficial to reducing immune resistance of insects on insecticidal fungi beauveria bassiana and accelerating knockout of fungus on hosts, so that the insecticidal fungi virulence is improved.

A material and a system are provided that efficiently inactivate a molting hormone by means of a protein having ecdysteroid 22-oxidase activity.

The invention discloses a method for establishing an ecdysone-enriched ajuga multiflora bunge suspension cell line. The method comprises the following steps: 1, placing an ajuga multiflora bunge explant on an induction culture medium for induction culture to obtain a first callus; 2, placing the first callus on a subculture medium for carrying out subculture for more than 5 times to obtain a second callus; 3, placing the second callus on a suspension culture medium for suspension shake culture; and 4, harvesting cells and extracting ecdysone. By the method disclosed by the invention, the ajuga multiflora bunge suspension cell line is firstly established and haploid plants having a full set of parental genes can be quickly and efficiently obtained. The method is conductive to deeply analyzing the mechanism for synthesizing ecdysone from ajuga multiflora bunge from the perspective of cytology and lays the technical foundation for the genetic transformation and genetic modification of ajuga multiflora bunge.

The invention provides an azadirachta indica cell suspension culture medium and a cell suspension culture method. According to the azadirachta indica cell suspension culture medium, based on a basic culture medium, component matching is optimized, and a certain amount of sodium vanadate, L-cysteine, tyrosine, nicotinamide, dithiothreitol, beta-D-galactose, cyclohexanol phosphate, diethylaminoethylhexanoate, 6-BA, ecdysone, sodium nitroprusside, docusate sodium, flaccid knotweed herb juice and henry munronia herb juice is added; the cell suspension culture method comprises the following stepsof 1) obtaining of sterile test-tube plantlets; 2) induction and subculture for calluses; 3) cell suspension culture. By adopting the culture medium and the culture method, not only can the stable azadirachta indica cell suspension culture system be obtained, but also the biomass of suspension cells and the content of azadirachtin in the suspension cells can be significantly improved, the technical support is provided for azadirachtin production through large-scale azadirachta indica cell culture, and important application value is achieved.

The invention relates to a large specification Eriocheir sinensis culture high-yield method comprising the following steps: firstly, determining a pond used for culturing Eriocheir sinensis; secondarily, inputting Eriocheir sinensis seeds into the culture pond, wherein females and males are separately cultured; thirdly, stop feeding compound pellets and animal baits after 17th exuviations of the Eriocheir sinensis, using botanical baits, and stop inputting quicklime and calcium dihydrogen phosphate containing rich calcium and phosphor substances; fourthly, feeding animal baits with added ecdyson after feeding botanical baits for 15 days, and applying calcium dihydrogen phosphate into the culture pond. The method is simple and easy in operation, and can effectively improve Eriocheir sinensis culture average mu yield and average specification by 10%.

The invention discloses a unilaterally-substituted phenyl urea compound (as shown in formula I) and an application thereof. The structural formula of the compound is as shown in the formula I in description, wherein R1, R2 and R3 in the formula I represent hydrogen, C1-C4 alkyl, C1-C4 halo-alkyl, C1-C4 alkoxy, C1-C4 alkyl sulfenyl, halogen, nitryl, cyan and hydroxyl. The compound VS01 disclosed by the invention has an obvious ecdysone antagonistic activity on 20E and also has a certain killing activity on lepidopteron; the compound is simple in structure and large in modification space, and can be used for in-depth study as an ecdysone antagonist type lead compound with a totally new structure.

The invention relates to the technical field of microbes, in particular relating to a genetically engineered virus I (SpltMNPV-Delta egt-egfp) capable of preventing and controlling farming and forestry pests such as cotton leafworm. The virus is preserved in the China Center for Type Culture Collection (CCTCC) with the collection number of CCTCC NO: V201027. In the invention, a genetic engineering method is utilized to delete and recombine the wild virus so as to obtain the cotton leafworm genetically engineered virus. The genome of the virus loses an egt gene, namely an ecdysteroid uridine diphosphate glucosyltransferase gene; and a marker gene, namely an enhanced green fluorescent protein (egfp) gene is inserted to the locus losing the egt gene, wherein the marker gene is controlled by the wild virus polyhedrin (ph) promoter. Compared with the wild virus, the genetically engineered virus has the advantages of higher pesticidal speed, better field application effect and the like.

A topically admimstrable vasodilator formulation comprises arginine and / or one or more derivatives thereof, black pepper extract and / or one or more components or derivatives thereof; and peppermint extract and / or one or more components or derivatives thereof. The formulation may further comprise rosemary oil, a penetration enhancers, an ecdysteroid and / or one or more other agents that enhance the vasodilator activity of the formulation. The valodilalor formulation may be suitable for treating or preventing a disease, disorder or condition associated with a decrease and / or impairment of blood flow in a subject by topically administration to the subject.

The invention relates to a method for expressing and purifying a silkworm ecdyson receptor EcR / USP protein compound. The method comprises the following steps: constructing a USP-pET-28a (+) recombinant vector and an EcR-pET21b-MDX12 recombinant vector; converting the USP-pET-28a (+) recombinant vector and the EcR-pET21b-MDX12 recombinant vector into an expression strain, culturing, and carrying out inducible expression to obtain a recombinant target compound; and purifying the obtained recombinant target compound through nickel ion affinity chromatography and molecular sieve chromatography. The recombinant expression vector EcR-pET21b-MDX12 and USP-pET-28a (+) are constructed, and are co-converted to induce co-expression of two proteins in order to obtain a large amount of the recombinant target protein compound, and the recombinant target protein compound undergoes nickel ion affinity chromatography column and molecular sieve separation and purification in order to obtain the highly-pure target protein.

A food supplement for restoring male sex drive (libido) comprises in a daily dose a set of L-arginine, rutin from pollen or beebread, decenic acids from drone brood, zinc from a zinc compound, and vitamin B6. It also comprises icariin from horny goat weed, saponins from true or false ginseng root, and ecdysteroids from Leuzea or crowned saw-wort, in various combinations thereof.

The invention discloses a biological insecticide screening mode, which comprises the following steps: integrating acceptor EcR with ecdysone GAP with gene expressing box Promoter-EcR-AOX1 TT and super spiral protein USP gene with gene expressing box TEF1 Promoter-USP-CYC1 TT on the colorant layer of Pasteur pichia; integrating DNA fragment with fruit fly 5XEcRE-HSP27 Promoter-GFP on the colorant layer yeast; getting the product. To co-culture screening drug with this product, we can get stopping or reinforcing GFP expression biological insecticide.

The invention discloses an ethanol extract (PDE4) and total saponin (PDE5), which have anticancer activity, from paris dulongensis H. Li&Kurita, preparation methods of the ethanol extract (PDE4) and the total saponin (PDE5) and application of the ethanol extract (PDE4) and the total saponin (PDE5) to the preparation of a medicine for treating or preventing leukemia, a liver cancer, a lung cancer, a mammary cancer and a colon cancer, and provides a separation and identification method of chemical components in the PDE 5. Polyphyllin V, polyphyllin I, beta-ecdysone, protodioscin, parisaponin I and ginsenoside Rg1 are separated from the PDE5 and identified by adopting a separation method of chromatographic column chromatography and semi-preparative high performance liquid chromatography and an identification method of a mass spectrum and a nuclear magnetic resonance wave spectrum. The separation and identification method is used for clarifying main chemical components of the PDE5 for the first time, can be used for simply ad quickly obtaining the chemical components from the PDE5 through a provided method, and is used for reconstructing a quality control standard for the paris dulongensis H. Li&Kurita, the extract PDE4 of the paris dulongensis H. Li&Kurita or the total saponin PDE5 of the paris dulongensis H. Li&Kurita.

The present invention provides an ecdysteroid derivative in which a fluorescent moiety is attached to an ecdysteroid moiety by derivatisation of a hydroxyl group on the alkyl side chain of the ecdysteroid moiety. The derivative is capable of binding to an ecdysone receptor or ligand domain thereof and can be used in assays.

A biologically active food supplement intended to increase the athletes' muscle mass comprises Leuzea (or other medicinal plant containing ecdysteroids), male bee brood, ascorbic acid (Vitamin C), Vitamin E, and excipients (calcium stearate, talc, lactose) with the following ratio of components (mass %): Male bee brood 1-70; Leuzea 5-70; Ascorbic acid 1-70; Vitamin E 0.05-30; calcium stearate 0.92-0.95, talc 3-6, lactose—the rest.

A food supplement for restoring male sex drive (libido) comprises in a daily dose a set of L-arginine, rutin from pollen or beebread, decenic acids from drone brood, zinc from a zinc compound, and vitamin B6. It also comprises icariin from horny goat weed, saponins from true or false ginseng root, and ecdysteroids from Leuzea or crowned saw-wort, in various combinations thereof.

The present invention relates to flea ecdysone receptor and ultraspiracle proteins; to flea ecdysone receptor and ultraspiracle nucleic acid molecules, including those that encode such flea ecdysone receptor and ultraspiracle proteins; to antibodies raised against such flea ecdysone receptor and ultraspiracle proteins; and to compounds that inhibit flea ecdysone receptor and / or ultraspiracle activity. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising a protective compound derived from a protein of the present invention that inhibits the binding between ecdysone receptor and ecdysone as well as the use of such therapeutic compositions to protect animals from flea infestation.

The invention relates to the field of a microorganism technology, in particular relating to a prodenia litura genetically engineered virus which can prevent farming and forestry pests such as prodenia litura. The virus is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: V201028. In the invention, a genetic engineering method is employed to delete and restructure a wild type virus. The egt gene, namely the ecdysone uridine diphosphate glucosyltransferase gene is deleted from the genome of the virus; a BmKTal gene controlled by a wild type virus polyhedron gene (ph) promoter and a marking gene-enhanced green fluorescent protein (egfp) gene controlled by the wild type virus polyhedron gene (ph) promoter are inserted into the locus at which the egtgene is deleted. The virus provided by the invention has the advantages of faster pest killing speed than that of the wild type virus, less dosage effect, better field application effect and the like.

The invention discloses a method for extracting ecdysone from common cyanotis roots and the ecdysone thereof, belonging to the technical field of the extraction and the separation of effective components of plants. The method comprises the following steps of: decocting 0.5-1kg of common cyanotis roots by 10L of water, or extracting by using 1-95 percent methanol or ethanol, and the ratio of the using amount of the methanol or the ethanol to the common cyanotis roots is 5L-10L to 1kg; filtering, decompressing and concentrating to obtain liquid with the relative density of 1.05-1.20; regulatinga pH value to 6-10 by using alkali; then extracting by a mixed solvent with normal butanol and ethyl acetate, wherein the ratio of the normal butanol and the ethyl acetate is 4:6-0.5:9.5; washing, decompressing and concentrating to obtain a fluid extract with the relative density of 1.2-1.3; adding 50-95 percent methanol or ethanol for dissolving and dilution, passig through overhigh alkali type anion exchange resin, and eluting, concentrating and drying in a decompressing way to obtain the ecdysone. Compared with a conventional method, the method enhances the yield by 1.16-1.38 percent.