Genetically engineered virus of Chinese ballworm

A technology of genetic engineering and cotton bollworm, applied in the field of microorganisms, can solve the problems of affecting popularization and application, slow insecticidal speed, etc., and achieve the effects of improved insecticidal effect, fast insecticidal speed, and good field application effect

Inactive Publication Date: 2003-05-21
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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Problems solved by technology

But because it has the defect of slow insecticidal ...

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  • Genetically engineered virus of Chinese ballworm
  • Genetically engineered virus of Chinese ballworm
  • Genetically engineered virus of Chinese ballworm

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Embodiment Construction

[0012] 1. Embodiment

[0013] 1. Engineering virus No. 3 provided by the present invention has lost the egt gene in its genome, that is, the ecdysone uridine diphosphate glucosyltransferase gene, and at the loss of the egt gene site, it is inserted by the Chinese cotton bollworm virus basic DNA-binding protein Gene (p6.9) promoter and the insect-specific neurotoxin AaIT gene controlled by the polyhedrin gene promoter of the virus, and the marker gene β-galactosidase controlled by the heat shock protein gene (hsp70) promoter gene (LacZ). The source, effect, replacement and insertion site of the AaIT gene are the same as the prior art. The marker gene can also be replaced with the green fluorescent protein GFP gene, etc., or the marker gene can be deleted after the recombinant virus is constructed.

[0014] 2. No. 3 engineering virus provided by the invention, its construction method comprises the following steps:

[0015] Perform the first recombination to construct the reco...

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Abstract

A genetically engineered virus of Chinese ballworm is obtained by double recombination of wild virus. Its genome is deficient is egt gene. At the position deficient in egt gene, the insect-specific neurotoxic AaIT gene controlled by the promoters of Chinese ballworm virus' alkaline DNA binding protein gene (p6.9) and polyhedrin protein gene (ph) and the beta-galactosidase (LacZ) gene controlled by the promoter of heat shock protein gene (hsp70) are inserted. Its advantages are higher insecticiding speed and better application effect.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Chinese cotton bollworm genetic engineering virus capable of preventing and controlling agricultural and forestry pests such as cotton bollworm and tobacco budworm, that is, Genetically modified Helicoverpa armigera nucleopolyhedrovirus No. 3 ). The virus was deposited by the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms. The date of preservation was October 9, 1999, and the preservation number was CGMCCNO.0489. Background technique [0002] The Chinese cotton bollworm single nucleocapsidnucleopolyhedrovirus (Helicoverpa armigera single nucleocapsidnucleopolyhedrovirus, hereinafter referred to as wild-type virus or HaSNPV) has been used for the control of cotton bollworm and tobacco budworm. But because it exists the defective of desinsection speed slow, has influenced its popularization and application. In order to improve the ...

Claims

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Application Information

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IPC IPC(8): A01N63/00C12N7/01C12N15/12C12N15/63
Inventor 王华林陈新文孙修炼胡志红
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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