Genetically engineered virus of Chinese ballworm

Abstract

A genetically engineered virus of Chinese ballworm is obtained by double recombination of wild virus. Its genome is deficient is egt gene. At the position deficient in egt gene, the insect-specific neurotoxic AaIT gene controlled by the promoters of Chinese ballworm virus' alkaline DNA binding protein gene (p6.9) and polyhedrin protein gene (ph) and the beta-galactosidase (LacZ) gene controlled by the promoter of heat shock protein gene (hsp70) are inserted. Its advantages are higher insecticiding speed and better application effect.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Chinese cotton bollworm genetic engineering virus capable of preventing and controlling agricultural and forestry pests such as cotton bollworm and tobacco budworm, that is, Genetically modified Helicoverpa armigera nucleopolyhedrovirus No. 3 ). The virus was deposited by the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms. The date of preservation was October 9, 1999, and the preservation number was CGMCCNO.0489. Background technique [0002] The Chinese cotton bollworm single nucleocapsidnucleopolyhedrovirus (Helicoverpa armigera single nucleocapsidnucleopolyhedrovirus, hereinafter referred to as wild-type virus or HaSNPV) has been used for the control of cotton bollworm and tobacco budworm. But because it exists the defective of desinsection speed slow, has influenced its popularization and application. In order to improve the ...

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Problems solved by technology

But because it has the defect of slow insecticidal speed, it has affected its popularization and application.

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