Insect control sheet

Abstract

Provided is an insect control sheet using a polyhedrin protein polyhedron (Cry polyhedron) in which the insecticidal protein (Cry toxin) produced by Bacillus thuringiensis is fixed. The insect control sheet contains the Cry polyhedron, and is used by floating on water. The insect control sheet floats on the surface of water, and is obtained by layering a pure matrix layer (20) and a toxin-containing matrix layer (30) containing a Cry polyhedron (51) onto the undersurface of a sheet-shaped first sheet substrate (10). The pure matrix layer (20) comprises an easily decomposable or water-soluble second material, while the toxin-containing matrix layer (30) comprises the Cry polyhedron and an easily decomposable or water-soluble third material. The Cry polyhedron is gradually released from the toxin-containing matrix layer into the water on which the control sheet is floating.

Description

technical field

[0001] The present invention relates to a pest control sheet used by floating on water. Background technique

[0002] An insecticidal protein produced by Bacillus thuringiensis (hereinafter referred to as "Cry toxin") exhibits insecticidal activity against certain insects. The Cry toxin produced by Bacillus thuringiensis sisserovarisraelensis is known to be toxic to mosquito larvae.

[0003] The inventors immobilized Cry toxin in polyhedron protein polyhedrons, which are protein microcrystals, prepared by Bombyxmoricypovirus (BmCPV). Hereinafter, the polyhedron to which the Cry toxin is immobilized is referred to as "Cry polyhedron". That is, a tag for immobilizing the Cry toxin to the polyhedrin protein polyhedron is added to the N-terminal of the Cry toxin. Next, the tagged Cry toxin is immobilized on the polyhedrin protein polyhedron.

[0004] The inventor et al reported

[0005] (1) Cry polyhedron has insecticidal activity against mosquito larvae, ...

Examples

Embodiment Construction

[0058] Hereinafter, the control sheet, the control material and the transfer sheet according to the embodiment of the present invention will be further described with reference to the accompanying drawings. In this specification, in order to facilitate understanding of the present invention, some of the components and the like are schematically shown with reference to each drawing. Therefore, dimensions, ratios, and the like between constituent elements may differ from the actual ones.

[0059] In addition, in the cross-sectional view showing the stacked state, the spaces between layers are provided in the drawings for the purpose of explaining the stacked state clearly. In actual control sheets and controls, the layers are in contact with each other.

[0060] Furthermore, unless otherwise specified, the dimensions, materials, shapes, and relative positions of members and parts described in the embodiments of the present invention are merely illustrative examples, and are not...

technical field

[0001] The present invention relates to a pest control sheet used by floating on water. Background technique

[0002] An insecticidal protein produced by Bacillus thuringiensis (hereinafter referred to as "Cry toxin") exhibits insecticidal activity against certain insects. The Cry toxin produced by Bacillus thuringiensis sisserovarisraelensis is known to be toxic to mosquito larvae.

[0003] The inventors immobilized Cry toxin in polyhedron protein polyhedrons, which are protein microcrystals, prepared by Bombyxmoricypovirus (BmCPV). Hereinafter, the polyhedron to which the Cry toxin is immobilized is referred to as "Cry polyhedron". That is, a tag for immobilizing the Cry toxin to the polyhedrin protein polyhedron is added to the N-terminal of the Cry toxin. Next, the tagged Cry toxin is immobilized on the polyhedrin protein polyhedron.

[0004] The inventor et al reported

[0005] (1) Cry polyhedron has insecticidal activity against mosquito larvae, ...

Examples

Embodiment Construction

[0058] Hereinafter, the control sheet, the control material and the transfer sheet according to the embodiment of the present invention will be further described with reference to the accompanying drawings. In this specification, in order to facilitate understanding of the present invention, some of the components and the like are schematically shown with reference to each drawing. Therefore, dimensions, ratios, and the like between constituent elements may differ from the actual ones.

[0059] In addition, in the cross-sectional view showing the stacked state, the spaces between layers are provided in the drawings for the purpose of explaining the stacked state clearly. In actual control sheets and controls, the layers are in contact with each other.

[0060] Furthermore, unless otherwise specified, the dimensions, materials, shapes, and relative positions of members and parts described in the embodiments of the present invention are merely illustrative examples, and are not...

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