Newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and preparation method thereof

A dual inactivated vaccine, a technology for chicken Newcastle disease, which is applied in the field of dual inactivated chicken Newcastle disease and H9N2 subtype avian influenza vaccine and its preparation, can solve the problems of low cross-immunization protection rate, large immune dose, low antibody level, and the like, Achieve the effect of no formaldehyde residue, high antibody level and long duration

Active Publication Date: 2014-05-21
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For Newcastle disease and H9N2 subtype avian influenza, there are corresponding inactivated single vaccines and double inactivated vaccines, but the immune dose is large, the antibody level is low, and the cross-immunity protection rate against epidemic strains is not high. brings certain risks

Method used

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  • Newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and preparation method thereof
  • Newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and preparation method thereof
  • Newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Isolation and Identification of Example 1 H9N2 Subtype Avian Influenza Virus HN03 Strain

[0025] 1. Disease data

[0026] The disease material is aseptically collected heart blood, liver, brain and lung tissues of dead sick chickens.

[0027] 2. Tissue contacts

[0028] The disease materials were used as heart blood smears and viral tissue contact slides of various organs, and stained microscopic examination was performed, and the staining microscopic examination results of viral tissue contact slides were negative.

[0029] 3. Bacterial Culture

[0030] The above disease materials were cultured with Martin broth and sheep blood culture medium (prepared according to the method in the appendix of the current "Chinese Veterinary Pharmacopoeia"), and the results showed no colony growth, indicating that the disease materials did not contain bacteria.

[0031] 4. Treatment of disease materials

[0032] Cut and grind the above disease material in a sterile container, an...

Embodiment 2

[0058] Example 2 Preparation of Chicken Newcastle Disease and H9N2 Avian Influenza Dual Inactivated Vaccine

[0059] 1. Source of poisonous seeds for seedling production:

[0060] The virus species used to manufacture this product are chicken Newcastle disease virus La Sota strain and avian influenza virus A / chicken / He Nan / 03 / 2009 / (H9N2) strain CGMCC NO: 6258, of which avian influenza virus A / chicken / He Nan / 03 / The 2009 / (H9N2) strain is abbreviated as the H9N2 subtype avian influenza virus HN03 strain.

[0061] Chicken Newcastle Disease Virus La Sota strain (China Veterinary Drug Control Institute strain number: CVCC AV1615 strain) was purchased from China Veterinary Drug Control Institute, H9N2 subtype avian influenza virus HN03 strain was obtained from the National Veterinary Biological Products Engineering Technology Research Center according to Example 1 The method was isolated, identified by the Harbin Veterinary Research Institute, and submitted to the General Microbi...

Embodiment 3

[0099] Example 3 Double-dose Inoculation Safety Test of Chicken Newcastle Disease and H9N2 Avian Influenza Dual Inactivated Vaccine

[0100] Prepare 5 batches of chicken Newcastle disease and H9N2 avian influenza dual inactivated vaccine according to Example 2 (batch numbers: 20100301, 20100502, 20100603, 20110104, 20110305 ） , before inactivation, the antigen content of Newcastle disease virus La Sota strain and avian influenza (H9 subtype) HN03 strain were both 10 8.0 EID 50 / 0.3ml / feather portion.

[0101] The research objects were 7-day-old SPF chickens (purchased from Beijing Merial Weitong Experimental Animal Co., Ltd.) and 7-day-old Sanhuang broiler chickens (purchased from Nanjing Shifosi Chicken Farm). After each batch of chickens was bought back, they were adapted for a few days to check the general condition and clinical disease status of the chickens. Weak chickens were eliminated and healthy chickens were selected to enter the test. Five batches of double-dos...

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Abstract

The invention provides a newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and a preparation method of the bivalent inactivated vaccine, which relate to the field of biological engineering. The bivalent inactivated vaccine comprises a newcastle disease virus LaSota strain and an avian influenza virus A / chicken / HeNan / 03 / 2009 / (H9N2) strain. The preparation method of the vaccine comprises the steps of preparing, concentrating and deactivating virus liquid, performing water phase preparation and oil phase preparation, and emulsify, thereby obtaining the bivalent inactivated vaccine. The newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine has good immunogenicity, creates a high antibody level, and can withstand not only the attack of homologous viruses, but also the attack of JX02 and SH01 of H9N2 subtype avian influenza viruses, which are prevalent since 2011; the bivalent inactivated vaccine can create high level antibodies and has a long persistent period; and the preparation method of the bivalent inactivated vaccine is simple and safe.

Description

Technical field [0001] The present invention involves the field of biological engineering, and specifically involves a chicken new city epidemic and H9N2 subtypen avian influenza dioxide vaccine and its preparation methods. Background technique [0002] New City's epidemic, also known as Asian chicken plague or pseudo -chicken plague, has a high incidence and mortality. It is a major infectious disease that endangers the poultry industry. OIE lists it as a Class A disease.Newcastle Disease (ND) is a kind of acute, thermal, sewage, and high contact infectious infectious diseases caused by the Newcastle Disease Virus (NDV)., Bleeding of mucous membranes and paste.The disease rate is high, and the chicken farming industry is seriously harmful.In 1926, it was first discovered in Indonesia, and soon found in the new city of the United Kingdom that countries around the world were recorded. [0003] Avian Influenza Virus (AIV) belongs to the genus A influenza virus of the Ai Virus, beca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/14A61P31/16A61K39/145A61K39/17
Inventor 于漾揭鸿英朱亚露何家惠邓碧华张小飞艾玉春刘志凌顾巍巍
Owner JIANGSU ACAD OF AGRI SCI
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