Method for preparing poultry cholera microcapsule vaccine

A technology for microcapsules and fowl cholera, which is applied in the field of bioengineering and achieves the effects of convenient use, long storage period and scientific method

Inactive Publication Date: 2009-12-09
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a preparation method of fowl cholera microcapsule vaccine for the existing shortcomings of fowl cholera vaccine, and introduce microcapsule technology to solve the problems in the production practice of fowl cholera vaccine

Method used

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  • Method for preparing poultry cholera microcapsule vaccine
  • Method for preparing poultry cholera microcapsule vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] After rejuvenating the freeze-dried strains of Pasteurella multocida type A, select typical colonies and culture them to make seed liquid, use the flat bottle solid culture method to obtain semi-finished products with a bacterial count of more than 12 billion / ml, and pass the inactivation test Then mix with sodium alginate solution for primary emulsification, then mix this emulsion with chitosan solution for secondary emulsification, dehydrate the final emulsion through a spray dryer, and make microcapsule vaccine dry powder, each gram of vaccine contains bacteria The body size is 350 billion, and the vaccine particle size ranges from 2-9um (see attached figure 1 ). The test chickens produced an immune response 3 days after being inoculated with the vaccine, and within 6 months of immunization, the average protection rate was 80.67%.

Embodiment 2

[0017] After the freeze-dried strain of Pasteurella multocida type A is rejuvenated, typical colonies are selected and cultured to make seed bacterial liquid, and the semi-finished product containing more than 9 billion bacteria / ml is obtained by deep aerated fermentation culture method, which is tested by inactivation Then mix with sodium alginate solution for primary emulsification, then mix this emulsion with chitosan solution for secondary emulsification, dehydrate the final emulsion through a spray dryer, and make microcapsule vaccine dry powder, each gram of vaccine contains bacteria The body size is 300 billion, and the vaccine particle size ranges from 1-12um (see attached figure 2 ). The test chickens produced an immune response 3 days after being inoculated with the vaccine, and within 6 months of immunization, the average protection rate was 76.32%.

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Abstract

The invention discloses a preparation method of fowl cholera microcapsule vaccine. The preparation method of the vaccine is to prepare the seed bacterial liquid after the freeze-dried strain of Pasteurella multocida type A is resuscitated and subcultured, and obtain a semi-finished product containing more than 9 billion bacteria / ml through culture and proliferation; the semi-finished bacterial liquid is sterilized After passing the biopsy test, it is homogenized with an equal volume of 2% sodium alginate solution through a disperser for primary emulsification; the above emulsion and an equal volume of 5% chitosan solution are subjected to secondary emulsification through a high-pressure emulsifier; the final product The emulsion is dehydrated by a spray dryer to make a microcapsule vaccine dry powder. The preparation method is scientific, simple and reasonable, the production is stable and reliable, and the product yield reaches more than 85%. Using microencapsulation technology, the finished product has the advantages of high antigen content, long sustained release time, low toxic and side effects, low cost, convenient use, and long shelf life.

Description

technical field [0001] The invention relates to a preparation method of fowl cholera microcapsule vaccine, which belongs to the technical field of bioengineering. Background technique [0002] Fowl cholera is a contact septic infectious disease caused by Pasteurella multocida that mainly attacks poultry (chickens, ducks, geese) and wild birds, with high morbidity and mortality. Among the measures to prevent fowl cholera, in addition to keeping the breeding environment clean, vaccine prevention has always been the main method to prevent and control fowl cholera. Although my country currently has vaccine products for the prevention of fowl cholera such as "fowl cholera propolis inactivated vaccine" and "G 190 Freeze-dried live vaccine " etc., but these vaccines still have many deficiencies: the one, cause various stress reactions (injection local inflammation, muscle necrosis, food and egg reduction, growth inhibition, etc.) after inactivated vaccine injection; Because some ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/102A61K9/50A61P31/04
Inventor 刘学贤龚建森吕晓娟
Owner JIANGSU INST OF POULTRY SCI
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