Preparation method and application of African swine fever virus P11.5 protein specific polyclonal antibody

A technology of African swine fever virus and polyclonal antibody, which is applied in the direction of antiviral immunoglobulin, viral peptide, immunoglobulin from serum, etc., can solve the problems of genome differences of ASFV isolates, and achieve less antigen usage and antigen purity High and low antigenicity effect

Active Publication Date: 2019-06-04
YANGZHOU UNIV
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Problems solved by technology

The virus is the only member of the ASFV genus of the African swine fever virus family, and currently has only one serotype, but there are certain differences in the genomes of ASFV isolates in different regions

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  • Preparation method and application of African swine fever virus P11.5 protein specific polyclonal antibody
  • Preparation method and application of African swine fever virus P11.5 protein specific polyclonal antibody
  • Preparation method and application of African swine fever virus P11.5 protein specific polyclonal antibody

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Embodiment Construction

[0017] Below in conjunction with specific embodiment, further elaborate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the application.

[0018] 1. Screening of peptide epitopes

[0019] According to the amino acid sequence of the African swine fever virus P11.5 protein published online by Medicine Online, a specific epitope with good hydrophilicity, strong antigenicity, and commonality was screened and designed, such as the sequence P11.5-1—DQEEKKALQNKETKNLGIP (SEQ IDNo.1 ), P11.5-2—PEERCTYKFNSYTKKMEL (SEQ ID No. 2).

[0020] 2. Synthesis of peptide epitopes

[0...

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Abstract

The present invention discloses a preparation method and an application of an African swine fever virus P11.5 protein specific polyclonal antibody. The method uses P11.5 polypeptide epitope immunological experimental animals of mice, rabbits, etc. to prepare the specific polyclonal antibody. The method has high antigen purity, the prepared antibody is strong in specificity and high in affinity, used antigen is small in amount, and operations are simple and convenient. The preparation method of the polyclonal antibody comprises steps of ASFV P11.5 specific antigen epitope selection, polypeptideantigen synthesis, animal immunity, antibody determination, etc. Compared with conventional methods, the preparation method is simple and rapid, and high in antigen purity, the prepared antibody is strong in specificity, and the used antigen is small in amount. The polyclonal antibody can be used for detection of African swine fever virus P11.5 protein and provides an important technical method for prevention and control of African swine fever in China.

Description

technical field [0001] The invention relates to a preparation method and application of an African swine fever virus P11.5 protein-specific polyclonal antibody. Background technique [0002] African swine fever, also known as East African swine fever or warthog disease, is an acute heat-type highly contagious infectious disease of pigs caused by African swine fever virus. The disease only infects pigs. The clinical symptoms and pathological changes are very similar to those of swine fever. The main manifestations are fever, skin cyanosis, and bleeding of lymph nodes, kidneys, and gastrointestinal mucosa. The mortality rate of this disease is very high, which can be as high as 100% during the epidemic. At present, African swine fever has been prevalent in more than 30 countries, and there is a tendency to continue to expand and spread. On August 3, 2018, the first African swine fever epidemic was confirmed in my country. The diagnosis of African swine fever often uses red b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/01C07K16/08C07K16/06G01N33/569
Inventor 秦爱建沈安宁周小钰钱琨邵红霞叶建强
Owner YANGZHOU UNIV
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