Primer group and kit for rapidly identifying respiratory tract microorganisms based on nanopore sequencing and application of primer group

Abstract

The invention discloses a primer group for detecting respiratory tract microorganisms based on a nanopore sequencing method. The nucleotide sequences of the primer group are as shown in SEQ ID NO:1-20. The microorganisms are streptococcus pneumoniae, staphylococcus aureus (resisting to methicillin), klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, stenotrophomonas maltophilia, candida albicans, haemophilus influenzae, legionella pneumophila, enterococcus faecium, chlamydia psittaci, cryptococcus gattii, aspergillus fumigatus and pneumocystis jiroveci. According to the invention, sequencing optimization is carried out on different types of samples of the respiratory tract microorganisms, so that the detection method, the kit and the like are suitable for various typesof respiratory tract samples; and a targeted amplification method is adopted, so that the detection requirements of sputum, alveolar lavage and other sample types can be met. In addition, parallel sequencing can be performed, so that the flux of the detected samples is increased, the sequencing time is shortened, and the contradiction between the flux of detected pathogenic species and the cost and time is further relieved.

Description

technical field [0001] The present invention relates to the technical field of detection of respiratory tract microorganisms, and more specifically, to a primer set, kit and application thereof for rapid identification of respiratory tract microorganisms based on nanopore sequencing. Background technique [0002] The disease caused by pathogenic microorganisms invading the respiratory tract and multiplying is called respiratory tract infection. There are many kinds of pathogenic microorganisms in respiratory tract infection, the symptoms of infection are similar, it is difficult to distinguish clinically, and co-infection of multiple pathogenic microorganisms often occurs. Since precise diagnosis and treatment urgently require the right medicine, the identification of multiple pathogenic microorganisms is required. [0003] The most direct way to identify the type of pathogenic microorganism is to detect the nucleic acid of the pathogenic microorganism. At present, the com...

AI Technical Summary

Problems solved by technology

The common point of the above patents is that the method of multiple fluorescent PCR or gene chip is used for detection, but the sequence of the pathogen cannot be analyzed. If the concentration of the pathogen is relatively low and close to the detection limit of the kit, the result at this time has certain uncertainty.

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