Fluorescence quantitative PCR (polymerase chain reaction) universal premier for detecting pathogenic aspergillus, detection probe and kit

A technology for detection kits and detection probes, applied in fluorescence/phosphorescence, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of lack of specificity, low positive rate, difficult to obtain materials, etc., and achieve high sensitivity High specificity and specificity, high specificity and sensitivity, and easy sampling
CN102321738AInactive Publication Date: 2012-01-18GUANGZHOU INST OF RESPIRATORY DISEASE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU INST OF RESPIRATORY DISEASE
Publication Date
2012-01-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a fluorescence quantitative PCR (polymerase chain reaction) universal premier for detecting aspergillus, detection probes and a detection kit, wherein the detection kit comprises universal premiers displayed by base compositions such as SEQ ID. NO: 1 and SEQ ID. NO: 2; and the detection probe is CY5-TAAAGTTGGGTGTCGGCTGG-BHQ aiming at aspergillus fumigatus, the detection probe is FAM-TTGATTTGCGTTCGGCAAGC-BHQ aiming at aspergillus flavus, the detection probe is HEX-ACAAGTTGCAAATAAATGCGTCG-BHQ aiming at aspergillus, and / or the detection probe is ROX-ATGGTTGGAAAACGTCGGCA-BHQ aiming at aspergillus Niger. The multiple PCR detection method, premier, the probes and the kit thereof provided by the invention aim at four main pathogenic aspergilli and have high specificity and sensitivity; and the detection method is rapid, simple and convenient, and can be used for detecting and identifying the strain of pathogenic bacteria.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a fluorescent quantitative PCR general primer, a detection probe and a kit for detecting pathogenic aspergillus. Background technique

[0002] In recent years, with the wide application of broad-spectrum antibiotics and immunosuppressants and the increasing number of immunocompromised populations such as hematological malignancies and organ transplants, the incidence of deep fungal infections is rising sharply. Among them, Aspergillus has become an important pathogenic fungus second only to Candida albicans in immunocompromised patients. Aspergillus infection occurs in up to 50% of immunocompromised patients. Aspergillus infection, especially invasive aspergillosis, has a poor prognosis. Although new antifungal drugs are emerging and used in clinical treatment, the fatality rate is still as high as 50% to 90%. Due to the non-specific clinical manifestations of the disea...

Claims

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