Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus subtilis strain and application of antifungal metabolite in grain storage

A technology of Bacillus subtilis and Bacillus, applied in the fields of application, preservation of edible seeds, and methods based on microorganisms, can solve problems such as limited application development potential, low practical application value, and limited antibacterial spectrum, and achieve high application development potential , easy to large-scale production, wide antibacterial spectrum effect

Inactive Publication Date: 2017-05-10
HENAN UNIVERSITY OF TECHNOLOGY
View PDF11 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Bacillus subtilis antimicrobial peptide announced by Chinese patent (application number 200510038629.6) only has the effect of inhibiting Aspergillus flavus, the antibacterial spectrum is narrow, and the practical application value is low; the Bacillus subtilis LPL-NM45 announced by Chinese patent (application number 201310263918.0) only has Inhibition of Aspergillus niger, Fusarium moniliforme and Penicillium oxalicum in corn storage, with limited antibacterial spectrum and limited potential for application and development

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus subtilis strain and application of antifungal metabolite in grain storage
  • Bacillus subtilis strain and application of antifungal metabolite in grain storage
  • Bacillus subtilis strain and application of antifungal metabolite in grain storage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation, screening and identification of Bacillus LM3403

[0022] Bacterial colonies with obvious morphological differences were isolated and purified from the wild yak manure samples grown on the alpine non-polluted grassland above 3000 meters above sea level by dilution coating method, numbered and stored for future use.

[0023] The plate confrontation method was used to screen the bacterial strains with broad-spectrum antagonistic ability against mold from the bacterial strains obtained from the above separation and purification, and the strain with the strongest broad-spectrum antagonistic ability was obtained, which was named LM3403.

Embodiment 2

[0024] Embodiment 2: Bacillus LM3403 species identification

[0025] Refer to the "Common Bacteria System Identification Manual" for strain morphological observation and physiological and biochemical characteristics analysis. The colony of strain LM3403 on the NA plate is round, opaque and milky white, sticky, and the edges are irregular; the cells are rod-shaped, Gram-positive, spore-shaped, and oval. The colony of strain LM3403 on the NA plate is round, opaque and milky white, sticky, and the edges are irregular; the cells are rod-shaped, Gram-positive, spore-shaped, and oval. Aerobic growth, exercise, positive V-P test, positive catalase test, positive indole test, positive starch hydrolysis, positive casein hydrolysis, positive gelatin liquefaction, positive glucose fermentation test, positive nitrate reduction, positive urea utilization. The morphological characteristics, physiological and biochemical characteristics of the strain LM3403 were consistent with the standard...

Embodiment 3

[0028] Embodiment 3: Bacterial strain LM3403 antibacterial spectrum

[0029] Using Fusarium graminearum, Fusarium moniliforme, Aspergillus flavus, Aspergillus niger, Aspergillus gray-green, Aspergillus fumigatus, Penicillium oxalicum, Penicillium extensa, Mucor tallum and other main harmful molds for grain storage as indicator bacteria, the confrontation culture method was used to determine the strains The broad-spectrum antibacterial ability of LM3403, the results are shown in Table 2.

[0030] Table 1 Antibacterial spectrum of strain LM3403

[0031]

[0032] Note: "+" indicates that the antibacterial bandwidth is 5mm

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of grain storage, and discloses a bacillus subtilis strain having broad-spectrum antagonism for main grain storage hazard-moulds, a preparation method of an antifungal metabolite of the bacillus subtilis strain, and application of the bacillus subtilis strain and the antifungal metabolite in grain storage. The strain is bacillus subtilis LM3403, and has a preservation number of CGMCC No. 9377. The bacillus subtilis LM3403 has a better inhibiting effect for the main grain storage hazard-moulds such as fusarium graminearum, fusarium moniliforme, aspergillus flavus, aspergillus niger, aspergillus glaucus, aspergillus fumigatus, penicillium oxalicum, penicillium expansum and mucor mucedo, and is wide in antibacterial spectrum, thus having a giant application potential. The antifungal metabolite of the bacillus subtilis LM3403 has an obvious inhibiting effect for the growth of the main grain storage hazard-moulds. The storage test results prove that after the antifungal metabolite of the bacillus subtilis LM3403 is added into stored grains, the growth of the moulds can be effectively inhibited in a grain storage process, so that the grain storage safety is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of grain storage, and in particular relates to a Bacillus strain broad-spectrum antagonizing major grain-harming molds, a preparation method for its antibacterial metabolite, and an application of the Bacillus strain and its antibacterial metabolite in grain storage. Background technique [0002] Compared with other countries in the world, my country's grain storage period is longer, with an average storage period of 3 years. In long-term storage, stored grains will inevitably be affected by external environmental factors and moisture migration in grain piles, resulting in mold growth, metabolism, and toxin production. The growth and metabolism of mold can decompose the nutrients in the grain and cause the quality of the grain to deteriorate to varying degrees. According to incomplete statistics, the annual loss caused by grain mildew in the world alone accounts for more than 3% of the total output. In my c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04A23B9/28C12R1/125
CPCA23B9/28C12P1/04C12N1/205C12R2001/125
Inventor 陈亮王殿轩卞科
Owner HENAN UNIVERSITY OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com