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Compound fungicide FX for degrading straws and application thereof

A technology for compounding bacteria and straw, applied in the directions of bacteria, fungi, microorganism-based methods, etc., can solve the problems of high-temperature compost decomposing, and achieve the effect of promoting decomposing and decomposing, realizing recycling and broad application prospects.

Active Publication Date: 2016-06-22
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, during the composting process, the decomposition of macromolecules such as lignin, cellulose, and hemicellulose, and the increase in the temperature of the compost itself lead to the loss of function of many strains that have the function of degrading lignocellulose, which is the main factor that limits the maturity of high-temperature composting.

Method used

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  • Compound fungicide FX for degrading straws and application thereof
  • Compound fungicide FX for degrading straws and application thereof
  • Compound fungicide FX for degrading straws and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Screening of rice straw degrading strains:

[0050] The samples of degrading bacteria come from mixed organic materials at 50°C-70°C in the high temperature stage of industrial composting of Nanjing Mingzhu Fertilizer Co., Ltd., Baguazhou, Nanjing, Jiangsu Province. Weigh 10 g of the mixed sample, pass it through a liquid enrichment medium with rice straw powder as the only carbon source for one week (the liquid enrichment medium is: 2 g of rice straw powder dried to constant weight, crushed and passed through a 20-mesh sieve, added without Bacterial water 90ml) enrichment, after 2 days of enrichment, draw 10ml of enrichment solution and inoculate it into a new liquid enrichment medium to continue to cultivate the second generation, and so on to the third generation, draw 0.1ml to dilute and spread on On the Congo red medium, culture at 50°C for 3-7 days, select the strains that grow faster, especially those that can produce transparent circles, for isolation and purifi...

Embodiment 2

[0058] Example 2 Preparation of Rice Straw Degradation Compound Bacteria FX

[0059] Inoculate Bacillus subtilis strain NJX501, Bacillus subtilis strain NJX502, and methylotrophic Bacillus strain NJXy on LB medium separately, temperature 50°C, rotation speed 170rpm, shake culture for 1-2 days, remove supernatant after centrifugation Take the precipitate from the solution, and then resuspend it with 30ml of 0.9% sterilized normal saline to obtain the bacterial bacterium liquid, adjust the bacterial OD value of the bacterial bacterium liquid to 0.5; inoculate the Aspergillus fumigatus strain NJZ501 on the PDA medium, and culture it statically at 50°C For 4-7 days, shake with 20ml of 0.9% sterilized normal saline, and filter the culture to get the number of fungal spores 10 4The spore suspension of each / ml; the above-mentioned 3 bacterial bacterial liquids and fungal spore suspensions are mixed in equal volumes to obtain the compound bacterial agent FX.

[0060] LB medium is: pe...

Embodiment 3

[0062] Example 3 Determination of FX Enzyme Activity of Rice Straw Degradation Compound Strain

[0063] The bacterial bacterium liquid of 3 bacteria that embodiment 2 makes, the spore suspension of fungus, compound bacterial agent FX, respectively inoculate in the Erlenmeyer flask that 100ml rice stalk section culture medium is housed according to volume ratio 1%, 50 Under the condition of 170rpm shaking culture at 170rpm for 7 days and 15 days, samples were taken, and the fermentation broth was centrifuged at 8000rpm / min and 4°C for 5min; the crude enzyme liquid was obtained after filtering to remove bacteria and solid impurities, and the enzyme activity of filter paper (FPase) and cellulase ( CMCase) and xylanase activity (Xylanase).

[0064] Definition of enzyme activity: The definition of enzyme activity refers to the international standard, that is, the amount of enzyme that catalyzes the hydrolysis of the substrate to generate 1 μmol reducing sugar within 1 minute under ...

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Abstract

The invention discloses a compound fungicide FX for degrading straws. The compound fungicide FX comprises a Bacillus subtilis strain NJX501, a Bacillus subtilis strain NJX502, a Bacillus methylotrophicus strain NJXy and an Aspergillus fumigatus strain NJZ501. The compound fungicide is prepared by mixing the bacterium liquid of NJX501 with a bacterium OD value of 0.5-1, the bacterium fluid of NJX502 with a bacterium OD value of 0.5-1, the bacterium liquid of NJXy with a bacterium OD value of 0.5-1 and NJZ501 spore suspension with spore number of not less than 104 / ml in equivalent volume. The compound fungicide FX can be used for promoting the composting and decomposition of rice straws under high temperature conditions.

Description

technical field [0001] The invention belongs to the technical field of agricultural intensive production, and relates to a compound bacterial agent FX for degrading straw and its application. Background technique [0002] With the rapid development of my country's social economy and the popularization of fossil energy such as oil and natural gas, the traditional use of straw as the main fuel has gradually disappeared; at the same time, with the scale and intensification of agricultural production, a large number of straw resources have been discarded, which not only Occupying cultivated land and seriously wasting biomass resources, especially straw burning leads to environmental pollution and fires. [0003] Studies have shown that rice straw and other straw-like biomass resources contain a large amount of lignin, cellulose, and hemicellulose, as well as a large number of elements such as nitrogen, phosphorus, and potassium, and are comprehensively rich in nutrients; straw ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F11/08C05F15/00C12R1/125C12R1/07C12R1/68
CPCC05F11/00C05F11/08C12N1/14C12N1/20
Inventor 徐阳春沈大春韦中沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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