Complex microbial inoculant for controlling root-knot nematodes of crops and preparation method of complex microbial inoculant

A technology of root-knot nematodes and compound bacterial agents, applied in botany equipment and methods, microbial-based methods, biochemical equipment and methods, etc., can solve the problems of residual pesticide damage and environmental pollution, and achieve ingenious and reasonable formulas and effective control Good, eliminate the effect of mutual antagonism

Active Publication Date: 2019-12-17
四川郎三实业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, the soil is disinfected with lime nitrogen, and abamectin is used to prevent and control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Incline strain preparation:

[0027] Bacillus subtilis, Bacillus sphaericus, Pseudomonas fluorescens, Bacillus thuringiensis, Brevibacillus sporogenes, Paenibacillus polymyxa, and Pasteurella puncture were respectively cultured on the "beef extract peptone solid" medium for mother slant. For test tube culture, set the temperature at 32°C to 34°C, and cultivate for 2-3 days for later use.

[0028] Paecilomyces lilacinus, Verticillium chlamydoides, Beauveria bassiana, and Aspergillus fumigatus were cultured on the nutrient medium of "Maling potato sucrose solid" respectively, and the temperature was set at 28°C-30°C. 3--5 days for standby.

Embodiment 2

[0030] Seed culture:

[0031] The Bacillus subtilis, Bacillus sphaericus, Pseudomonas fluorescens, Bacillus thuringiensis, Brevibacillus spp., Bacillus polymyxa, and Pasteurella puncture parent species inclined test tubes cultivated in Example 1 were under aseptic conditions, respectively. Inoculate in a sterilized 500ml Erlenmeyer flask for culture. There is beef extract peptone broth medium in the bottle, and the volume of each bottle is 200ml; the temperature is set at 32°C, and the culture is carried out in a shaker at 200r / min for 3 days .

[0032] The Paecilomyces lilacinus, Verticillium chlamydoides, Beauveria bassiana, and Aspergillus fumigatus parent species test tubes cultivated in Example 1 were respectively inoculated in sterilized 500ml Erlenmeyer flasks in the potato sucrose liquid medium For culturing, the liquid volume in each bottle is 200ml; the temperature is set at 30°C, the shaker is set at 160r / min, and the time is 3 days.

Embodiment 3

[0034] Fermentation tank mixed culture:

[0035] 1: Fermentation tank compound fermentation medium composition (weight ratio):

[0036] Molasses 3%; corn starch 4%; soybean meal powder 2%; glucose 0.5%; peptone 0.2%; ferrous sulfate 0.008%; ammonium nitrate 0.3%; yeast extract 0.1%; Bran 3%; potassium dihydrogen phosphate 0.02%; calcium carbonate 0.1%; superphosphate 0.1%; sodium chloride 0.2%; magnesium sulfate 0.02%; sodium carbonate 0.8%; manganese sulfate 0.003%; defoamer 0.02%.

[0037]2: the microbial proportioning of composite fermentation culture (by the weight ratio of bacterial liquid in the Erlenmeyer flask obtained in embodiment two): Bacillus subtilis 10%; Bacillus sphaericus 5%; Pseudomonas fluorescens 10%; Bacillus thuringiensis 10% ; Brevibacillus spp. 5%; Paenibacillus polymyxa 10%; Pasteurella puncture 10%; Paecilomyces lilacinus 10%; %.

[0038] 3: Put the compound fermentation medium and each microbial liquid into the fermenter at the same time, and then...

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Abstract

The invention relates to the field of biologically-derived insecticidal preparations, in particular to a complex microbial inoculant for controlling root-knot nematodes of crops and a preparation method of the complex microbial inoculant. The complex microbial inoculant for controlling the root-knot nematodes of the crops and the preparation method of the complex microbial inoculant are characterized in that microorganism is composed of bacillus subtilis, bacillus sphaericus, pseudomonas fluorescens, bacillus thuringiensis, brevibacillus laterosporus, paenibacillus polymyxa, pasteurella pneumoniae, paecilomyces lilacinus, verticillium chalamydosporium, beauveria bassiana and aspergillus fumigatus. The novel multi-bacteria same-pot fermentation technology is adopted, the formula is clever and reasonable, the disadvantages of mutual antagonism in the liquid deep fermentation culture of compound bacteria are eliminated, the compound bacteria has high activity, and the complex microbial inoculant for controlling the root-knot nematodes of the crops has a good control effect on tomatoes and cucumber root-knot nematodes.

Description

technical field [0001] The invention relates to the field of biological source insecticide preparations, in particular to a compound bacterial agent for controlling root-knot nematodes of crops and a preparation method thereof. Background technique [0002] Vegetable root-knot nematode is a worldwide disease. There are four main types of root-knot nematodes that harm vegetables: root-knot nematode incognita, root-knot nematode northern, root-knot nematode peanut, and root-knot nematode Javan. The diseases of tomato and cucumber root-knot nematodes are mostly caused by the root-knot nematode incognita, and the damage is serious. According to many years of experimental surveys in Shandong, Guangxi, Henan and other places: the total incidence rate of tomato and cucumber greenhouses reached 67.6%, the total diseased plant rate was close to 50%, and the average incidence rate of old greenhouses was close to 95%. The diseased part is mainly parasitic on the fibrous roots and late...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/04A01N63/00A01P5/00C12R1/07C12R1/125C12R1/39C12R1/01C12R1/79C12R1/645C12R1/68
CPCC12N1/20C12N1/14A01N63/00Y02W30/40
Inventor 杨建设张元元
Owner 四川郎三实业发展有限公司
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