Bacillus cereus for activating soil nutrient and application of bacillus cereus

A technology of Bacillus cereus and soil nutrients, applied in the direction of application, soil conditioning materials, bacteria, etc., to achieve the effects of increasing diversity, promoting sustainable use of soil, and saving mineral resources

Active Publication Date: 2013-06-26
广东润华环保产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the activated nutrient bacteria currently studied are aerobic bacteria, and there are also a few reports on anoxic bacteria, but the activated nutrient bacteria that can efficiently improve soil fertility and promote crop growth under both aerobic and anoxic conditions have not been reported yet.

Method used

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  • Bacillus cereus for activating soil nutrient and application of bacillus cereus
  • Bacillus cereus for activating soil nutrient and application of bacillus cereus
  • Bacillus cereus for activating soil nutrient and application of bacillus cereus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The isolation, purification and identification of embodiment 1 bacillus cereus NC-1

[0030] (1) Separation

[0031] Prepare insoluble inorganic phosphorus medium: glucose 10.0g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, ferrous sulfate heptahydrate 0.03g, manganese sulfate tetrahydrate 0.03g, yeast extract 0.4g, phosphoric acid Calcium 5g, distilled water 1L, pH value 7.0~7.5.

[0032] The soil collected from the paddy field of South China Agricultural University in Guangzhou City, Guangdong Province was used as the screening soil sample, and 10.0g of the soil sample to be tested was accurately weighed by the flat plate coating method, and put into a 250mL triangular flask filled with 90mL sterile water (add glass beads), shake the shaker for 30 minutes to fully disperse the microorganisms, let it stand for 20~30s, and take the supernatant for 10-fold decreasing dilution, using 10 3 ~10 5 Dilution: Use a pipette gun to pipette 0.1mL of ...

Embodiment 2

[0036] Example 2 Characterization

[0037] (1) Morphological characteristics of bacteria

[0038] a-8 bacteria are Gram-positive bacteria with spores, and the bacterium does not expand after the formation of spores. The bacteria are rod-shaped, non-capsulated, motile, and aerobic. Gram staining effect see figure 1 , the cells were stained blue-purple, and the spores were not colored. Spore staining effect see figure 2 , the thallus is yellow-brown, and the spores are light blue.

[0039] (2) Colony Morphological Characteristics

[0040] The colonies formed on the nutrient agar medium are round or nearly round, soft, non-pigmented, and slightly glossy white colonies.

[0041] (3) Molecular biological characteristics

[0042]Bacterial total DNA was extracted using the Mobio PowerSoil DNA Isolation Kit (Bio-rad). Using bacterial 16S rDNA universal primers F63 (CAG GCC TAA CAC ATG CAA GTC, SEQ ID NO: 2) and R1087 (CTC GTTGCG GGA CTT ACC CC, SEQ ID NO: 3), routine PCR a...

Embodiment 3

[0043] Example 3 Effect experiment of NC-1 bacteria producing water-soluble phosphorus under insoluble inorganic phosphorus medium culture

[0044] Put 50mL of sterilized insoluble inorganic phosphorus medium (concentration of calcium phosphate is 5.0g / L) into the conical flask, add a-3, a-4, a -5, a-6, a-7, a-8 (that is, NC-1 bacteria) and a-9 bacteria (inoculum size 5 rings), after making the bacteria solution, culture it on a shaking table at 28°C and 70rpm for 7 days. Centrifuge the cultured bacterial solution at 10,000rpm and 4°C for 15min, take the supernatant and use the molybdenum antimony anti-colorimetric method to measure the content of water-soluble phosphorus (the unit is mg / L, which means the mg of water-soluble phosphorus contained in each liter of sample) number), the culture medium without any bacteria was set as the control, and each treatment was repeated 3 times. The test results are shown in Table 1 and image 3 . The results showed that the water-sol...

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Abstract

The invention discloses a bacillus cereus for activating soil nutrient and application of the bacillus cereus. The bacillus cereus is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 21, 2012 with preservation number of CGMCC No. 6127, and the 16SrDNA nucleotide sequence is shown by SEQ ID No: 1. According to the bacillus cereus, the soil nutrient can be obviously activated, the effectiveness of phosphorus and nitrogen in soil can be improved, the composition of a soil microbial community can be improved, the diversity and the evenness of the soil microbial community can be improved, the metabolic activity of the soil microbe can be improved and the like, the soil fertility can be improved, the crop growth can be promoted, the bacillus cereus is good for the agricultural sustainable development and utilization, and the bacillus cereus is wide in application prospect in the production practice.

Description

Technical field [0001] The invention involves a microorganism and its application, which specifically involves a waxybacteria ( Bacillus Cereus ) NC-1 and it promotes the application of crop growth in active soil nutrients, improve soil fertility, improve soil micro-ecological structure. Background technique [0002] During the growth of crops, the main nutritional elements, such as N, P, K, mostly absorbed through the soil through the root system, N, P, K sufficient nutrition supply is to restrict its potential production capacity and exert the ecology of ecology, exert the ecology of ecology,One of the main restrictions of efficiency.In order to meet the N, P, K and other nutritional elements required for plant survival, industrial fertilizer has always been considered the main way to achieve this goal.However, as the amount of fertilizers continues to increase, the negative impact is increasingly obvious, such as high cost, large consumption of non -renewable energy, pollution...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C09K17/00C12R1/085C09K101/00
Inventor 蔡燕飞李永涛孙礼勇黄娟孔德颖赵凤仪
Owner 广东润华环保产业有限公司
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