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Bacillus subtilis BC-198, and selenium-rich microbial inoculum and application thereof

A technology of Bacillus subtilis and BC-198, which is applied in the field of microorganisms, can solve the problems of single function and low selenium utilization, and achieve the effects of simple operation, increased selenium content, and improved quality of agricultural products

Active Publication Date: 2013-01-23
HUBEI HENO BIOLOGICAL ENG CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the present invention, the conversion of current bacterial agents to selenium or the use of a single strain or similar strains, the conversion of selenium and the utilization of selenium by plants are still relatively low
Moreover, most of the functional microbial fertilizers that are popularized and used, such as nitrogen fixation, potassium decomposing, phosphorus decomposing, decay promoting, growth promoting, and disease resistant fertilizers, are common microbial bacterial agents with a single function.

Method used

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  • Bacillus subtilis BC-198, and selenium-rich microbial inoculum and application thereof
  • Bacillus subtilis BC-198, and selenium-rich microbial inoculum and application thereof
  • Bacillus subtilis BC-198, and selenium-rich microbial inoculum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and identification of selenium-enriched multifunctional strains

[0033] From selenium-enriched tobacco waste in Baiyangping, Enshi City, Hubei Province, by adding 0.15% NaSeO 3 , 0.6% glucose, 0.5% sucrose, 0.8% peptone, 0.3% yeast extract, 0.4% sodium chloride, and 100 ml of water for enrichment, isolation and purification. Antagonism test and growth promotion test screened out a strain BC-198. The fermentation liquid of this strain increased the germination rate and root length of soybean seeds by 30-40% compared with the control; the inhibition zone for Fusarium and Penicillium was greater than 1.5 cm. It also has protease and cellulase activity; the morphological or physiological and biochemical characteristics of the strain are: Gram staining positive, cell rod-shaped, diameter figure 1 As shown, spores are formed, no swelling, no parasporium crystals; catalase, oxidase positive, aerobic growth, VP test positive, VPPH7 test negative, methyl red...

Embodiment 2

[0035] Example 2 Compatibility Test of Bacillus subtilis BC-198 and Nitrogenus Bacteroides

[0036] (1) Test medium composition: 1) 0.6% glucose, 0.5% sucrose, 0.8% peptone, 0.3% yeast extract, 0.4% sodium chloride water 100 ml, pH7.4; (2) Add 1.8% agar

[0037] (2) Cultivation of Bacillus subtilis BC-198 and Azotobacter sphaeroides

[0038] Put 50 ml of the above culture solution in a 250 ml Erlenmeyer flask, sterilize at 0.1 MPa for 25 minutes, insert Bacillus subtilis and Azotobacter sphaeroides respectively, place on a shaker, and culture at 28-30°C for 14-20 hours with shaking for later use.

[0039](3) Antagonism test

[0040] Prepare 100 ml of solid medium according to the above formula, sterilize it at 0.1 MPa for 25 minutes, add 1 ml of the 14-hour pre-cultivated nitrogen-fixing bacteria culture medium when it is cooled to 45 ° C, mix well, draw 15 ml and transfer it into a culture medium with a diameter of 9 cm Place the agar dish on a horizontal table top, cool i...

Embodiment 3

[0041] The preparation of embodiment 3 selenium-enriched bacterial agents

[0042] (1) The strain used in the selenium-enriched agricultural microbial agent: Bacillus subtilis BC-198;

[0043] The composition of the seed medium for cultivating Bacillus subtilis BC-198: Add 0.5% glucose, 0.3% starch, 0.6% peptone, 0.25% yeast extract, 0.4% sodium chloride, and phosphoric acid to 100 ml of selenium-containing solution (0.249 mg / ml) Dipotassium Hydrogen 0.1%, PH7.2;

[0044] Prepare seed culture medium according to the above formula, fill 100 milliliters in a 500 milliliter triangular flask, sterilize under 0.11MPa pressure for 25 minutes, after cooling to room temperature, pick the mother seed of Bacillus subtilis BC-198 bacteria with an inoculated bamboo shovel, inoculate Put it into the culture medium of the Erlenmeyer flask, and shake it at a constant temperature of 28°C ± 1°C for 14 hours on a shaker with a rotation speed of 190 rpm to obtain a Bacillus subtilis seed liquid...

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Abstract

The invention relates to Bacillus subtilis BC-198, and a selenium-rich microbial inoculum and application thereof. The collection number of the Bacillus subtilis BC-198 is CCTCC No.M2012321. The invention also provides a composite microbial inoculum of which the effective ingredients are the Bacillus subtilis BC-198 and Azotobacter chroococcum AS1.212. The preparation method of the selenium-rich microbial inoculum provided by the invention can utilize the existing universal fermentation plant, is simple to operate and has the advantage of low manufacturing cost; the prepared product has multiple functions of nitrogen fixation, growth promotion, fungus disease control and the like for crops; and after being applied to the crops, the selenium-rich microbial inoculum can increase the yield, improve the quality of the agricultural product, and enhance the selenium content in the agricultural product by more than 20 times, so that the agricultural product reaches the selenium-rich product quality standard.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis BC-198 and its selenium-enriched microbial agent and application. Background technique [0002] Selenium is an essential trace element for humans and animals. It has anti-cancer and anti-cancer effects. Long-term selenium deficiency can cause many diseases and is one of the causes of Keshan disease. Now people realize the necessity of supplementing selenium, and supplementing selenium through food is considered the safest and most convenient means. [0003] Like most countries in the world, my country is also a selenium-deficient area. There are more than 20 selenium-deficient provinces and cities in my country, and the selenium content in the soil is about 0.03-0.1mg / kg. Supplementing selenium to the soil is an effective way to obtain selenium-enriched agricultural products (food). Over the years, people have used selenium-enriched ore, selenium-enri...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/00A01N63/02A01P21/00A01P3/00C12R1/125C12R1/065
Inventor 陈家任刘金龙
Owner HUBEI HENO BIOLOGICAL ENG CO LTD
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