Method for producing efficient protein feed by feed enzymes cooperated with bacillus coagulans

A technology for Bacillus coagulans and protein feed, which is applied in the field of biotechnology fermentation to achieve the effects of high peptide content and soluble protein content, strong stress resistance and low dry matter loss

Active Publication Date: 2017-07-25
南宁精成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few research reports on improving the nutritional value and utilization rate of vegetable protein (mainly soybean meal) by utilizing the synergis...

Method used

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  • Method for producing efficient protein feed by feed enzymes cooperated with bacillus coagulans
  • Method for producing efficient protein feed by feed enzymes cooperated with bacillus coagulans
  • Method for producing efficient protein feed by feed enzymes cooperated with bacillus coagulans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Implement as follows: the following components are calculated by mass percentage

[0045] (1) Preparation of Bacillus coagulans seed solution from different sources: pick one ring of Bacillus coagulans slant, inoculate it into a 250mL Erlenmeyer flask filled with 100mL LB liquid medium, cultivate it on a shaking table at 37°C, 180r / min for 6h, and make seeds liquid;

[0046] (2) Bacillus coagulans liquid culture: medium formula: yeast powder: 15g / L, sodium chloride: 5g / L, maltose: 8g / L, starch: 15g / L, magnesium sulfate: 0.8g / L, manganese sulfate : 0.2g / L, disodium hydrogen phosphate: 1g / L, sodium dihydrogen phosphate: 3g / L, initial pH value: 7.4. When cultivating, the liquid volume of the fermentation tank is 40%, the volume of seed liquid is 3%, the rotation speed is 160r / min, and the ventilation volume is 0.6m 3 / h, cultivated at 35°C for 18h;

[0047] (3) Solid-state culture of Bacillus coagulans: material (soybean meal: bran = 1:9) and the bacterial solution obta...

Embodiment 2

[0059] Implement as follows: the following components are calculated by mass percentage

[0060] (1) Preparation of Bacillus coagulans (Bacillus coagulans GIM1.646) seed solution: pick 1 ring of Bacillus coagulans slant, inoculate it into a 250mL Erlenmeyer flask containing 100mL LB liquid medium, shake the table at 37°C, 180r / min Cultivate for 6 hours to make seed solution;

[0061] (2) Bacillus coagulans liquid culture: medium formula: yeast powder: 30g / L, peptone: 10g / L, sodium chloride: 1g / L, disodium hydrogen phosphate: 5g / L, maltose, starch, magnesium sulfate, Manganese sulfate content design test (see Table 2), initial pH value: 6.8. When cultivating, the liquid volume of the fermentation tank is 20%, the seed liquid volume is 2%, the rotation speed is 200r / min, and the ventilation volume is 0.6m 3 / h, cultivated at 39°C for 20h;

[0062] Table 2 Design experiment of content of maltose, starch, magnesium sulfate and manganese sulfate

[0063] Test No. A M...

Embodiment 3

[0074] Implement as follows: the following components are calculated by mass percentage

[0075] (1) Preparation of Bacillus coagulans (Bacillus coagulans GIM1.646) seed solution: pick 1 ring of Bacillus coagulans slant, inoculate it into a 250mL Erlenmeyer flask containing 100mL LB liquid medium, shake the table at 37°C, 180r / min Cultivate for 6 hours to make seed solution;

[0076] (2) Bacillus coagulans liquid culture: medium formula: yeast powder: 25g / L, peptone: 5g / L, sodium chloride: 2g / L, maltose: 6g / L, starch: 20g / L, magnesium sulfate: 1.2 g / L, manganese sulfate: 0.3g / L, disodium hydrogen phosphate: 3g / L, sodium dihydrogen phosphate: 5g / L, initial pH value: 7.2. When cultivating, the liquid volume of the fermentation tank is 60%, the seed liquid volume is 1%, the rotation speed is 120r / min, and the ventilation volume is 0.6m 3 / h, cultivated at 37°C for 15h;

[0077] (3) Solid-state culture of Bacillus coagulans: material (soybean meal: bran = 1:9) and the bacterial...

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Abstract

The invention discloses a method for producing efficient protein feed by feed enzymes cooperated with bacillus coagulans, and belongs to the field of biotechnology fermentation. The bacterium enzymes are used for treating bean meal in a cooperated way; the sterilization treatment on the bean meal is not needed; the facultative anaerobic and anaerobic fermentation are performed in the whole process; the energy consumption is low; the dry substance loss is low. The technical flow process operation is simple and convenient; the method is suitable for direct production and feeding in a culture plant and is also suitable for scaled production in a feed plant; in addition, the raw material cost is low; the method is suitable for industrial production. The fermented bean meal has high acid fragrance; the palatability is high; the pH is 3.2 to 4.5; the organic acid content is 2.8% or above; the acid dissolution protein is improved by 6.5 times or above through being compared with that before the fermentation; the soluble protein with the relative molecular weight within 500 is 60% or above; the urease activity is lower than 0.073U/g; the cotton seed sugar content is lower than 0.42 percent; the stachyose is lower than 0.035 percent; the spore number of the bacillus coagulans is 2.0*10<9>cfu/g or above.

Description

technical field [0001] The invention belongs to the field of biotechnology fermentation, and in particular relates to a method for producing high-efficiency protein feed by cooperating with bacillus coagulans with feed enzymes. Background technique [0002] The absorption of macromolecular proteins by animals is obviously not as good as the absorption of small molecular polypeptides and amino acids. At the same time, there are many anti-nutritional factors in plant protein raw materials, such as urease, trypsin inhibitor, phytic acid, lectin, lipoxygenase and Soybean antigenic protein, etc. affect the digestion, absorption and metabolism of nutrients, and have adverse effects on the health and production performance of animals, thus restricting the more effective application of plant protein in poultry breeding. [0003] The current methods for producing high-efficiency biological protein feed mainly include protease hydrolysis and microbial degradation. [0004] The use of...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/14A23K10/37
CPCY02P60/87
Inventor 姚勇芳廖延智吴少微何秀婷谭才邓宋雪果魏强华朱美娟司徒满泉李平凡
Owner 南宁精成生物科技有限公司
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