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Bacillus vallismortis and application thereof

A technology of Bacillus dead valley and bacterial liquid, applied in the application, bacteria, fungicides and other directions, can solve the problems of small inhibition zone, difficult to meet the needs of agricultural production, weak bacteriostatic ability and so on

Active Publication Date: 2016-07-27
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It was preliminarily identified as Bacillus vallis smortis CZ by physiological and biochemical identification, which has a certain inhibitory effect on watermelon Fusarium wilt. It is difficult to meet the needs of agricultural production

Method used

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  • Bacillus vallismortis and application thereof
  • Bacillus vallismortis and application thereof
  • Bacillus vallismortis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Bacterial strain collection and the preparation of bacterial agent

[0031] 1. Obtaining of strains:

[0032](1) Sample collection: collect an appropriate amount of fresh plant samples from the roots of tomato plants in the vegetable base of Jiangsu Academy of Agricultural Sciences, Nanjing City, Jiangsu Province, rinse the dust and soil on the surface with tap water, dry naturally, and then sterilize the surface of the plant root tissue : Then soak in 75% alcohol for 1min and 8%NaClO for 4min in turn for surface disinfection treatment, and wash with sterile water for 4 times;

[0033] (2) Separation and screening: Cut the roots, stems and leaves with sterile scissors, cut the roots and stems into small pieces of about 1 cm, and place them on the LB plate; add water to the root and grind it into a paste, put it on the LB plate after standing for 10 minutes, and place it on the LB plate Incubate at 30°C for 48h;

[0034] (3) Purification: After the culture gr...

Embodiment 2

[0045] Example 2 Inhibition Test of Bacterial Strain WM005 to Watermelon Fusarium wilt

[0046] (1) Inoculate the watermelon Fusarium wilt bacteria on the PDA plate, cultivate the plate at 28°C, and then use a puncher with a diameter of 5mm to make a fungus cake. The WM005 bacterium liquid (concentration 10 8 cfu / mL, 100 μL), and at the same time, the plate only inoculated with pathogenic fungi was used as the control group, and each treatment was repeated three times;

[0047] (2) Place the PDA plate at 28°C for 7 days, wait until the pathogenic fungal colonies in the control group cover the plate, measure the diameter of the pathogenic fungal colony, and calculate the antibacterial rate according to the following formula:

[0048] Bacterial inhibition rate (%)=[(A-B) / (A-5)]*100%;

[0049] (Note: A is the diameter of the pathogenic fungal colony in the control group, B is the diameter of the pathogenic fungal colony in the treatment group).

[0050] (3) Antibacterial resul...

Embodiment 3

[0051] Embodiment 3WM005 bacterial strain colonization test in soil and watermelon bacterial strain

[0052] Add 1 μg / mL rifampicin to the LB plate, then inoculate the WM005 strain, gradually increase the concentration of rifampicin to 300 μg / mL, and screen out mutant strains that can grow stably and have the same physiological characteristics as the original strain; After 10 passages were transferred, the colony morphology was observed, and then transferred to LB medium containing 300 μg / mL rifampicin for labeling.

[0053] 1. WM005 colonization in soil

[0054] (1) Inoculate the strain WM005 labeled with 300 μg / mL rifampicin into LB culture medium, shake at 32°C, 180r / min, and shake for 24h to make a bacterial content of about 10 8 cfu / mL marker strain bacterial suspension;

[0055] (2) Put the natural soil (taken from Nanjing, the experimental field of Jiangsu Academy of Agricultural Sciences) and sterilized soil in flowerpots, 1kg of soil in each pot, inject 100mL of the...

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Abstract

The invention provides bacillus vallismortis WM005 with the preservation number being CCTCC NO: M2016020. The bacillus vallismortis can be stably planted in watermelon plants, thereby effectively preventing the occurrence of the fusarium wilt of watermelons. During use, a bacillus vallismortis liquid with the bacillus vallismortis content being 1, 010 cfu / mL can be prepared from the bacillus vallismortis for root irrigation of watermelon seedlings in fields, so that the purpose of preventing the fusarium wilt of the watermelons is achieved. The bacillus vallismortis agent is high in control effect, convenient to use and suitable for being applied and popularized in a large scale.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Bacillus vallismortis WMOO5 (Bacillus vallismortis WM005) and its application in the prevention and treatment of watermelon wilt Background technique [0002] Fusarium wilt (Blight) is a devastating disease of watermelon, which is widely distributed in watermelon producing areas in the world. With the adjustment of my country's agricultural industrial structure, the watermelon planting area is increasing, and continuous cropping obstacles have become an important factor restricting watermelon production. Fusarium wilt of watermelon is one of the main diseases of watermelon continuous cropping obstacle, and its pathogen is Fusarium oxyporum f.sp.niveum. With the continuous cropping and heavy cropping of watermelon year by year, the occurrence of watermelon wilt is becoming more and more serious, resulting in a large reduction in watermelon production, generally 30% to 40%...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 余向阳王琼张猛葛静程金金
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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