Compound fungicide and continuous fermentation method of compound microorganisms adopted

Abstract

The invention discloses a continuous fermentation method of compound microorganisms. The continuous fermentation method comprises the following steps: 1) respectively preparing a bacillus subtilis liquid, a bacillus licheniformis liquid and a clostridium butyricum liquid; 2) adding a basic fermentation liquid into a fermentation tank; 3) controlling the temperature of the basic fermentation liquid at 36-38 DEG C, controlling the pressure of the tank at 0.01MPa, firstly, inputting the bacillus subtilis liquid to cultivate, then, inputting the bacillus licheniformis liquid for continuous fermentation and cultivation and finally inputting the clostridium butyricum liquid to stand for anaerobic fermentation and cultivation; and 4) centrifugally concentrating the obtained fermentation liquid which is filtered in the step 3) to 28-32% of the original liquid in volume; and then spraying and drying to obtain a compound probiotic preparation. The method disclosed by the invention can be used for overcoming the defects that the number of sterilization and feeding in a single fermentation process of microorganisms are great, the fermentation procedure is simplified and meanwhile, the viable count is increased and the number of contaminating microorganisms is decreased by virtue of neutralizing synergistic effects of different microorganisms by continuous fermentation, so that the effect of microorganisms is improved.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and relates to a fermentation process of compound microorganisms—a staged continuous fermentation process of Clostridium butyricum, Bacillus licheniformis and Bacillus subtilis. Background technique [0002] Clostridium butyricum is an anaerobic Gram-positive bacillus. Because it is strictly anaerobic, if there is oxygen in the fermentation environment, the body of Clostridium butyricum will not grow or even die. Clostridium butyricum is resistant to heat, acid, and bile, and has strong tolerance to a variety of feed antibiotics, and can be used in combination; it can produce amylase, hydrolyze starch and sugars; produce butyric acid, acetic acid, and lactic acid, etc. metabolite. It has been widely used clinically to treat various intestinal diseases such as intestinal flora disorder, acute and chronic diarrhea, irritable bowel syndrome, and antibiotic-related enteritis caused by various r...

AI Technical Summary

Problems solved by technology

[0005] The separate fermentation of the above-mentioned probiotics faces problems such as high fermentation cost, low number of viable bacteria, and many miscellaneous bacteria, but there is no report on the triple fermentation technology at home and abroad.