Vesicles with inner and outer aqueous-phase gradient difference and preparation method and application thereof

A technology of gradient difference and vesicles, which is applied in pharmaceutical formulations, liposome delivery, drug delivery, etc., and can solve problems such as long time-consuming, low drug encapsulation rate, and limited drug loading range

Inactive Publication Date: 2011-03-30
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the above-mentioned shortcomings of current liposomes / vesicles as drug carriers and their methods for establishing ion gradient differences, such as limited range of drug loading, low drug encapsulation efficiency, long time-consuming, high cost, small gradient, etc., the present invention One object of the invention is to provide a vesicle with a gradient difference between the inner and outer water phases, the second object of the invention is to provide a method for preparing the vesicle, and the third object of the invention is to provide the application of the vesicle

Method used

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  • Vesicles with inner and outer aqueous-phase gradient difference and preparation method and application thereof
  • Vesicles with inner and outer aqueous-phase gradient difference and preparation method and application thereof
  • Vesicles with inner and outer aqueous-phase gradient difference and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Prescription HSPC 3g

[0095] CH 1g

[0096] PEG-CHS 1g

[0097] Hydration medium: 100ml of 300mmol citric acid solution (pH 4.00).

[0098] Preparation of blank liposomes: Weigh the prescribed amount of HSPC, CH (cholesterol), PEG-CHS (PEG molecular weight is 2000), and dissolve the membrane material with 10ml ethanol at 55°C to obtain the lipid phase; preheated to 55°C The hydration medium was injected into the lipid phase and incubated for 10 minutes to obtain the primary liposome product, which was then subjected to 20,000 psi high-pressure homogenization treatment to reduce the liposome particle size to 90nm, and passed through 0.8, 0.45, and 0.22 μm microporous membranes in sequence. That is, blank liposomes were obtained.

[0099] Establish gradient liposomes: Calculated according to the exchange capacity, 1.2ml (wet volume) anion resin (OH-) (717 type anion resin) can completely exchange 1ml of 300mmol / L citric acid solution. In this embodiment, 1 ml of blan...

Embodiment 2

[0104] Prescription DSPC 5g

[0105] 300mM citrate buffer 100ml

[0106] (1) Take an appropriate amount of chloroform to dissolve DSPC (distearoylphosphatidylcholine), and remove the chloroform by rotary evaporation to prepare a DSPC film. With 100ml 300mM citrate buffer as the hydration medium, the phospholipids are thinly hydrated to prepare DSPC large unilamellar liposomes (about 610nm), using sodium hydroxide solution (conventional method) or hydroxide type anion exchange resin (OH -) Adjust the pH of the external aqueous phase to 7 to establish a pH gradient. The liposomes whose gradient was established by sodium hydroxide solution was marked as A, and the liposomes whose gradient was established by hydroxide type anion exchange resin was marked as B. Microscopic observation revealed that the particle size of B was larger than that of A, and the PSS (Particle Sizing Systems) measurement results showed that the particle sizes of A and B were 596nm and 681nm, respectively...

Embodiment 3

[0110] Prescription: DSPC 3g

[0111] DSPE-PEG 2000 1g

[0112] Hydration medium: 200mM EDTA ammonium salt and 10mg / ml ammonium hyaluronate solution 100ml.

[0113] Prepare blank liposomes: weigh the prescribed amount of DSPC, DSPE-PEG 2000 , 55°C, dissolve the membrane material with 10ml ethanol to obtain the lipid phase; inject the hydration medium preheated to 55°C into the lipid phase, and incubate for 10 minutes to obtain the primary liposome, and then undergo 20,000psi high-pressure homogenization treatment, Reduce the particle size of liposomes to 100 nm, and pass through 0.8, 0.45, and 0.22 μm microporous membranes in turn to obtain blank liposomes.

[0114] Build gradient liposomes:

[0115] A mixed ion exchanger consisting of three ion exchangers: 331 weakly basic epoxy anion exchange resin, 122 weakly acidic phenolic cation exchange resin and DEAE-cellulose. The wet visual volume ratio of 331 weakly basic epoxy anion exchange resin and 122 weak acid phenoli...

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Abstract

The invention belongs to the field of pharmaceutical preparations and discloses vesicles with inner and outer aqueous-phase gradient difference. The vesicles are prepared by treating blank vesicles by adopting an ion exchange method or combining the ion exchange method and a dialysis method or an ultrafiltration method. The invention also provides a preparation method and application of the vesicles; and the preparation method comprises the following steps of: preparing the blank vesicles; treating the prepared blank vesicles by using an ion exchanger; and performing elution treatment so as to prepare the vesicles with inner and outer aqueous-phase gradient difference. The prepared vesicles can be mixed with medicinal solution to realize active medicament carrying so as to prepare the pharmaceutical preparations. The prepared preparations also can be subjected to ion exchange treatment or freeze-drying treatment. The provided vesicles have higher inner and outer aqueous-phase gradient difference, can realize ion gradient medicament carrying of the vesicles and achieve higher encapsulation rate; meanwhile, the preparation method is simple and is low in cost, and the vesicles can be better applied to preparing liposome gel, magnetic liposome and nanoparticles / nanogel.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular to a vesicle with a gradient difference between internal and external water phases, a preparation method and application thereof. Background technique [0002] Some amphiphilic molecules, such as many natural or synthetic surfactants and phospholipids that cannot simply associate into micelles, will spontaneously form a molecular orderly assembly with a closed double-layer structure when dispersed in water, called Vesicles (vesicles), also known as liposomes (liposomes). The meaning of the terms vesicle and liposome is somewhat ambiguous in the literature. It is generally believed that if these amphiphilic molecules are the natural surfactant lecithin, the resulting structure is called a liposome; if it is composed of a synthetic surfactant, it is called a vesicle. Therefore, liposome refers to a special class of vesicles formed by phospholipids, which is the first vesic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K9/00
Inventor 邓意辉马艳铃张小飞杨强宋阳黄微葳
Owner SHENYANG PHARMA UNIVERSITY
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