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Bacillus atrophaeus YL3 and application thereof

A technology of Bacillus rysophila and Bacillus, which is applied in the field of Bacillus fumeus YL3 and its application in the prevention and control of plant diseases, can solve the problems of unreported application of biocontrol, achieve rich biocontrol resources, good application prospects, The effect of reducing usage

Active Publication Date: 2016-03-23
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biocontrol application of Bacillus atrophaeus to strawberry anthracnose, powdery mildew and crop wilt has not been reported at home and abroad

Method used

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  • Bacillus atrophaeus YL3 and application thereof
  • Bacillus atrophaeus YL3 and application thereof
  • Bacillus atrophaeus YL3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Inhibitory Effect of Bacillus Fuesus YL3 on Bacteria

[0021] A. The pathogenic bacteria tested

[0022] The tested bacteria include Bacillus megaterium, Phytophthora amylovora and Bacterial spot of rice, provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences. Use LB culture solution (10.0 g of peptone, 5.0 g of yeast powder, 10 g of NaCl, 1000 mL of distilled water) at 28°C and 150 rpm for 2 days, and dilute to 10 with sterile water. 8 cfu / mL, spare.

[0023] B. Test method:

[0024] The Bacillus fuencus YL3 screened by the Jiangsu Academy of Agricultural Sciences was activated on LB medium, then transferred into 50mL LB medium, cultured with shaking at 28°C and 150rpm for 2 days, and diluted with sterile water to 10 8 cfu / mL, spare.

[0025] Take 5 μL of Bacillus fueci YL3 bacteria solution, spot it on the center of the LB plate, incubate at 28°C for 48 hours, spray with pathogenic bacteria diluent, incubate overnight at 28°C, and ...

Embodiment 2

[0030] Inhibitory Effects of Bacillus Fuesus YL3 on Five Kinds of Phytopathogenic Fungi

[0031] A. For testing pathogenic fungi:

[0032] Pathogenic fungi, including tomato fusarium wilt, rice sheath blight, rape sclerotinia, strawberry anthracnose and cabbage black spot fungus, were provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences. Use PDA medium (200g potato, 20g glucose, 20g agar and 1000mL distilled water, pH 6.5-7.0) to culture at 28°C for 5 days, set aside.

[0033] B. Test method:

[0034] The preparation of the Bacillus fuencus YL3 bacterial liquid was the same as that in Example 1.

[0035] Place the pathogenic fungus bacterium cake (6mm in diameter) on one side of the blank PDA plate, 30mm away from the edge of the plate. On the other side of the plate, 30 mm away from the edge of the plate, 5 μL of Bacillus fueceae YL3 bacterial solution was spotted. Cultivate at a constant temperature of 28°C, set up clean water as a cont...

Embodiment 3

[0043] Metabolic secretions of Bacillus fumigatus YL3-production of antimicrobial-related substances

[0044] Test materials: protease detection medium: skimmed milk powder 100g, agar 20g, dilute to 1000mL. Siderophilic detection medium: CAS60.5mg, 10mL ferric iron solution (1mmol L -1 FeCl 3 ·6H 2 O), HDTMA72.9mg, agar 20g, be settled to 1000mL, pH7.0. Chitinase detection medium: Chitin 15g, MgSO 4 ·7H 2 O0.5g, FeS0 4 ·7H 2 O0.01g, K 2 HPO 4 0.7g, KH 2 P0 4 0.3g, 20g agar, dilute to 1000mL, pH7.0-7.2. Cellulase detection medium: peptone 10g, yeast powder 10g, sodium carboxymethylcellulose 10g, NaCl 5g, KH2 PO 4 1g, 20g agar, dilute to 1000mL, pH7.0.

[0045] The preparation of the Bacillus fuencus YL3 bacterial liquid was the same as that in Example 1. In the center of each detection medium plate, 5 μL of Bacillus fueci YL3 bacterial solution was spotted, and each treatment was repeated 3 times. Incubate at a constant temperature of 28°C for 3-7 days and observ...

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Abstract

The invention relates to antibiological inoculant. The effective active component of the antibiological inoculant is bacillus atrophaeus YL3. The antibiological inoculant is characterized in that the antibiological inoculant is an aqueous solution and is prepared from, by weight, 89.5-99.8% of bacillus atrophaeus YL3, 0.1-10% of sodium dodecyl sulfate and 0.1-0.5% of potassium sorbate. During application, the aqueous solution is used for preventing and treating strawberry anthracnose and strawberry powdery mildew when sprayed on leaf surfaces and used for preventing and treating various types of crop wilt and the like when irrigated to roots. The antibiological inoculant has the advantages that by introducing bacillus atrophaeus YL3 which is safe to humans and livestock and has a good effect on preventing and treating diseases of strawberries and various plants, and the antibiological inoculant can prevent and treat plant diseases through biological agents; the dosage of chemical agents is reduced; meanwhile, the biological environment is improved, and the amount of chemical agents left in agricultural products is decreased.

Description

technical field [0001] The invention relates to a biocontrol fungus and its application, in particular to a strain of bacillus fuece YL3 and its application in preventing and treating plant diseases. Background technique [0002] With the adjustment of planting structure and the invasion of foreign pests, major crop diseases have frequently broken out in recent years. At present, the prevention and control of plant diseases in production mainly relies on chemical pesticides, which not only cause serious pollution to the ecological environment, but also directly increase the residues of toxic chemicals in agricultural products, causing serious harm to human health. In order to achieve the purpose of effectively preventing and controlling plant diseases and reducing the use of chemical pesticides, the methods of using biological control technology to control plant diseases have attracted more and more attention from governments and people of various countries. Biological cont...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00C12R1/07
Inventor 刘邮洲陈夕军梁雪杰乔俊卿
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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