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Micro-ecological preparation applied to culture of turbots

A technology of microecological preparations and turbot, applied in the field of microecological preparations, can solve problems such as unreasonable strain collocation, poor strains, and poor effects

Active Publication Date: 2017-05-31
青岛家宝生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a large number of probiotic feed additive products on the market have poor effects. The main reason is that the strains are poor and the combination of strains is unreasonable.

Method used

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  • Micro-ecological preparation applied to culture of turbots
  • Micro-ecological preparation applied to culture of turbots
  • Micro-ecological preparation applied to culture of turbots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Isolation and identification of Bacillus subtilis (DH077)

[0066] In the laboratory aseptic operation table, the intestinal tract of healthy turbot was taken for bacterial species isolation. The turbot intestines were chopped and homogenized. Suspended and mixed with an appropriate amount of normal saline, centrifuged for 5 min to collect the supernatant turbidity, then added an appropriate amount of normal saline, centrifuged at 8000g for 10 min, and collected the supernatant turbidity to obtain the test sample. Pipette 1ml of the supernatant liquid into a test tube containing 9ml of normal saline and vortex to mix, as 10 -2 Diluent, use the same method to dilute to 10 -3 , respectively take turbot intestinal stock solution, 10 -2 Diluent, 10 -3 0.1 ml of each of the dilutions was spread on a Bacillus subtilis separation plate and cultured at 37°C for 24 hours. On the separation medium, the colony characteristics of Bacillus subtilis are more typical, ...

Embodiment 2

[0067] Example 2: Application of Bacillus subtilis (DH077) in the cultivation of turbot

[0068] Bacillus subtilis (DH077) powder, viable count 1.0×10 8 cfu / g or more. Bacillus subtilis (DH074, isolated from Qingdao Donghai Pharmaceutical Co., Ltd.) powder, viable count 1.0×10 8 cfu / g or more. Bacillus subtilis (commercially purchased) powder, viable count 1.0×10 8 cfu / g or more.

[0069] The above three Bacillus subtilis powders were added to the turbot basal diet at a mass ratio of 1:100 to conduct a turbot breeding experiment.

[0070] 240 turbot were randomly divided into 4 groups with 3 repetitions in each group. Group I was the basic diet (control group); group II was the basic diet supplemented with 1% Bacillus subtilis (DH077) bacteria powder of the present invention. Group III was supplemented with 1% Bacillus subtilis (DH074) powder in the basic diet. Group IV was supplemented with 1% Bacillus subtilis (commercially purchased) bacteria powder in the basic diet...

Embodiment 3

[0076] Example 3: Isolation and identification of Bacillus licheniformis (DH136)

[0077] In the laboratory aseptic operation table, the intestinal tract of healthy turbot was taken for bacterial species isolation. The turbot intestines were chopped and homogenized. Suspended and mixed with an appropriate amount of normal saline, centrifuged for 5 min to collect the supernatant turbidity, then added an appropriate amount of normal saline, centrifuged at 8000g for 10 min, and collected the supernatant turbidity to obtain the test sample. Pipette 1ml of the supernatant liquid into a test tube containing 9ml of normal saline and vortex to mix, as 10 -2 Diluent, use the same method to dilute to 10 -3 , respectively take turbot intestinal stock solution, 10 -2 Diluent, 10 -3 0.1 ml of each dilution was spread on the Bacillus licheniformis separation plate, and cultured at 37°C for 24 hours. On the isolation medium, the colony characteristics of Bacillus licheniformis were more...

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Abstract

The invention relates to the field of feed additives, and discloses a micro-ecological preparation applied to culture of turbots. The micro-ecological preparation comprises bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae or lactobacillus acidophilus. The micro-ecological preparation is applied to increase of the growth rate of turbots, reduction of feed coefficients of turbots, and increase of the culture survival rate of turbots. The complex micro-ecological preparation can be separately used after being prepared into veterinary drugs and feed additives, and can also be directly fed after being mixed with culture feed of turbots, thus being obvious in application effects, and having good economic and social benefits.

Description

technical field [0001] The invention relates to the field of feed additives, in particular to a microecological preparation applied to turbot culture. Background technique [0002] Turbot, commonly known as "Turbot", is one of the world's recognized high-quality flounder. Turbot has high content of glial protein, delicious taste and rich nutrition. It has the functions of invigorating the kidney and brain, helping the yang and refreshing, as well as beauty and beauty. Long-term consumption can nourish fitness and improve people's disease resistance. It is a precious edible fish. , was introduced from the United Kingdom by the China Yellow Sea Fisheries Research Institute in 1992, and after the success of large-scale production in 1999, it has become an important object of marine aquaculture in the north. [0003] With the advent of the upsurge of turbot breeding, the problem of turbot breeding has also received widespread attention. In 2006, the overdose of antibiotics suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/18A23K20/28C12N1/20C12N1/18C12R1/125C12R1/10C12R1/865C12R1/23
CPCC12N1/18C12N1/20
Inventor 崔云龙庄盈婷李洪福
Owner 青岛家宝生物科技有限公司
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