Method for producing trichoderma solid spawn by taking amino acid hydrolytic solution and rice straw as raw materials

A technology of rice straw and solid strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of environmental pollution, irrational use of resources, etc., and achieve the effect of good quality and good nutrient conditions

Inactive Publication Date: 2016-11-09
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of environmental pollution caused by unreasonable utilization of rice straw and waste livestock and poultry and slaughtering leftovers resources, and to provide a solid strain of Trichoderma using rice straw and waste livestock and poultry amino acid hydrolyzate as raw materials. method, the obtained Trichoderma solid strain can be directly added to the decomposed organic fertilizer to produce high value-added Trichoderma bio-organic fertilizer

Method used

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  • Method for producing trichoderma solid spawn by taking amino acid hydrolytic solution and rice straw as raw materials
  • Method for producing trichoderma solid spawn by taking amino acid hydrolytic solution and rice straw as raw materials
  • Method for producing trichoderma solid spawn by taking amino acid hydrolytic solution and rice straw as raw materials

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Experimental program
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Effect test

Embodiment 1

[0040] 1. Observation of bacterial strains

[0041] The strain is Trichoderma guizhouense NJAU4742 (Trichoderma guizhouense), which has the following characteristics: Trichoderma guizhou is isolated from traditional Chinese medicine residue and composted, and belongs to the branch of Trichoderma harzianum in evolution; its hyphae can strongly parasitize Botrytis cinerea, Alternaria, Sclerotinia sclerotiorum, Rhizoctonia solani, Sclerotinia sclerotiorum and Fusarium oxysporum; Trichoderma guizhou has a unique light sensitivity, which increases the concentration of spores; in situ fluorescence studies show that Trichoderma guizhou can colonize soil and plant rhizosphere , promote plant growth, inhibit plant diseases, and increase crop yields.

[0042] 2. Culture of strains

[0043] 1) Inoculate the strain NJAU4742 into a solid medium PDA glass petri dish. The culture conditions are: 28°C, 7 days. Wash and scrape the green spores on the petri dish with 5 mL of sterile water, and...

Embodiment 2

[0046] 1. Preparation method of waste livestock and poultry amino acid hydrolyzate

[0047] (1) In a closed container, the sick and dead livestock and poultry animals are automatically crushed, and all solids and liquids are automatically transferred to a closed hydrolysis tank. After the initial acid concentration c (1 / 2H 2 SO 4 ) is 3-5mol L -1 , hydrolysis at 80-100°C and 1-2 atmospheric pressure for 2-5 hours;

[0048] (2) When the hydrolysis is completed and the solution in the hydrolysis tank is cooled to below 80°C, use a vacuum suction device that can enter and exit the feed port of the hydrolysis tank to float upright on the grease to separate the upper layer of grease;

[0049] (3) After the oil is separated, slowly add potassium hydroxide in the hydrolysis tank according to the mass ratio of the hydrolyzate to potassium hydroxide at 4:1, stir while adding, and adjust the pH value of the solution to 1-3; or press the hydrolyzate The mass ratio of lobster shells or...

Embodiment 3

[0064] Example 3 Guizhou Trichoderma NJAU4742 Solid Fermentation

[0065] After adding 10L of tap water into the large-capacity transfer box, add 285mL of waste livestock and poultry amino acid hydrolyzate to fully dissolve, then add 1.5Kg of crushed rice straw, stir well and soak overnight at room temperature (the straw can be softened and the straw can be treated with low pH conditions) Sterilize), adjust the pH of the stalk to 3.5 with solid KOH and a little amino acid stock solution after overnight, remove the raw materials that have adjusted the pH, adjust the water content to 75% and stir until fluffy, add 150g corn flour at the same time, fully stir Evenly get the fermentation substrate of Guizhou Trichoderma 4742, according to 20% (spore concentration 10 8 spore / mL, v / w) to inoculate Trichoderma Guizhou 4742 fermentation broth, spread the solid substrate in a shallow plate, the thickness of the straw is about 2cm-5cm, and place it in a dark room at 28°C-32°C for the se...

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Abstract

The invention discloses a method for producing trichoderma solid spawn by taking an amino acid hydrolytic solution and rice straw as raw materials. A filamentous fungus Trichoderma harzianum with a good plant rhizosphere growth-promoting effect is utilized, the rice straw rich in lignocellulose and abandoned livestock amino acid hydrolytic solution nutrients are fully utilized under the culture condition of 28 DEG C, rapid growth and spore production are achieved, after solid fermentation is conducted for 5 days to 6 days, the number of chlamydospores of trichoderma guizhouense NJAU4742 reaches up to 2.4*10<10> spore / g, the solid spawn is directly added into a decomposed organic fertilizer, a trichoderma amino acid bio-organic fertilizer of which the number of stable and effective living bacteria is larger than 2*10<8> can be rapidly produced, and the bio-organic fertilizer has a very good rhizosphere growth-promoting effect on tomatoes.

Description

technical field [0001] The invention belongs to the field of biological fertilizers, and relates to a method for producing solid Trichoderma strains by using amino acid hydrolyzate and rice straw as raw materials. Background technique [0002] Rice straw is an important agricultural resource, which can be used for livestock and poultry feed, papermaking raw materials, building materials, biomass energy and organic fertilizer raw materials, etc. my country's rice planting area reaches 30 million hectares 2 , rich in rice straw resources. Due to the low utilization rate, especially the unreasonable use of rice straw in most rural areas that is still discarded or burned, etc., it leads to a waste of resources and serious environmental pollution, which is not conducive to environmental protection, let alone sustainable ecological development. Over the past ten years, it has been confirmed that rice straw has absolute advantages and potential in soil fertilizer after being degr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05G3/00C12R1/885
CPCC12N1/14C05C11/00C05G3/00
Inventor 沈其荣刘东阳李荣刘秋梅王帅
Owner NANJING AGRICULTURAL UNIVERSITY
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