Microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof

A microbial inoculum and phytoremediation technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., to achieve the effects of increasing the number of effective viable bacteria, wide sources and long storage periods

Inactive Publication Date: 2010-06-16
INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem of the present invention is to overcome the shortcomings of the existing phytoremediation technology and provide a microbial agent for strengthening phytoremediation of arsenic-contaminated sites and its preparation and application method, which can greatly improve the remediation efficiency of centipede grass and expand the application ability of phytoremediation technology

Method used

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  • Microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof
  • Microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof
  • Microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 strengthens the preparation of the bacterium agent of restoration

[0029] (1) Strain fermentation:

[0030] a. Primary slant cultivation

[0031] Peptone 0.5%, beef extract 0.3%, sodium chloride 0.5%, agar 1.5%, pH 7.2, put the culture medium into a test tube; sterile; inoculate Agrobacteriumsp. ;

[0032] b. Secondary Erlenmeyer flask liquid culture

[0033] In step a, remove the agar from the main material of the first-level slant culture medium, as the main material of the second-level culture, the pH is 7.2, the culture medium is packed into a triangular flask, after sterilization and cooling, the slant bacteria solution is inoculated into the liquid medium; Shake culture at 28°C for 24 hours, terminate the culture, and set aside;

[0034] c. Three-stage fermenter fermentation

[0035] The main material of the culture in the fermenter is the same as the main material of the secondary culture. Put the secondary culture liquid into the fermenter acc...

Embodiment 2

[0042] Embodiment 2, indoor implementation of microbial bacterial agent

[0043] Indoor pot experiments were carried out with the bacterial agent prepared by the above method. The effective amount of viable bacteria in the bacterial agent is 10 9 ~10 12 pcs / g.

[0044] The specific implementation method is: apply 5 g of bacterial agent in 1 kg of high-arsenic soil, mix evenly, put it into a pot, and transplant the centipede grass seedlings. Harvest two months after planting.

[0045] From figure 2 It can be seen that the dry weight of the upper part of the centipede grass reaches 2.1 g after the application of the bacterial agent, which is 16% higher than that of the control.

[0046] From such as image 3 It can be seen that the arsenic enrichment in the upper part of the centipede grass reaches 3.1 mg, which is 41% higher than that of the control.

[0047] From the above experimental results, it can be seen that the application of arsenic-resistant bacterial agents s...

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Abstract

The invention provides a microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof, belonging to the technical field of environment restoration engineering. The strain utilized by the bactericide is Agrobacteriumsp. C13. The bactericide adopts a composite carrier which comprises turfy soil, saw dust, rice hulls and active carbon with the mass percentage being 30:25-30:25-30:15; the microorganism fermentation liquor is mixed with the composite carrier according to the mass ratio of 0.5-1:1; and the adsorption amount of the microorganisms of the composite carrier reaches 109-1012 per gram. The prepared bactericide is applied to the arsenic-polluted fields restored by ciliate desert-grass according to the use amount of 5g/kg, the biomass liveweight of the overground part of the restored plants is improved by 16-17%, and the removal rate of arsenic in the fields is improved by 40-41% in comparison. The carrier of the bactericide in the invention has rich raw materials, simple preparation method and easy realization of large-scale production.

Description

technical field [0001] The invention relates to a high-efficiency microbial agent and its preparation and application method, in particular to a microbial agent capable of promoting the arsenic super-accumulator-Centipeda to absorb soil arsenic, and belongs to the technical field of environmental restoration engineering. Background technique [0002] Arsenic compounds have carcinogenic, teratogenic and mutagenic properties. Arsenic poisoning is a systemic disease mainly caused by the skin, which can harm the skin, respiratory, digestive, urinary, cardiovascular, nervous, and hematopoietic systems of the human body. Due to the exploitation of arsenic-contaminated groundwater, the use of arsenic-containing pesticides and fertilizers, metal smelting and mining, a large amount of land in my country has been severely polluted by arsenic. Arsenic in the soil environment enters the human body through food, drinking water and breathing, and exceeding the limit will cause arsenic po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/00B09C1/00B09C1/10C12R1/01
Inventor 廖晓勇阎秀兰朱岗辉
Owner INST OF GEOGRAPHICAL SCI & NATURAL RESOURCE RES CAS
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