Preparation method of composite solid-state probiotic agent including phellinus linteus

A probiotic agent, the technology of Phellinus linteus, applied in the application, animal feed, animal feed and other directions, can solve the problems of insufficient decomposition and utilization of nutrients, unable to produce ideal effects, and low content of beneficial microorganisms, so as to improve the palatability of feeds. Sex, ease of operation, the effect of regulating immunity

Active Publication Date: 2017-10-17
INNER MONGOLIA UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Composite solid probiotics are a kind of animal feed and additive products that have been researched more at present. At present, conventional bacterial fermentation is a product prepared by single or multiple bacterial strains for one-time fermentation. The content of beneficial microorganisms is small, and The decomposition and utilization of nutrients in t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This embodiment is a method for preparing a composite solid probiotic containing Phellinus igniarius. The method uses Phellinus igniarius seed liquid inoculated into a solid fermentation medium for fermentation to degrade cellulose and hemicellulose. Sugar; then add the post-fermentation broth in 2 times, carry out a sequential post-fermentation process, obtain the fermentation product after the fermentation, and dry the fermentation product to obtain a composite solid probiotic. The post-fermentation bacteria liquid is Bacillus, Saccharomyces cerevisiae and lactic acid bacteria.

[0028] The mass parts of each component in the solid fermentation medium are: 20 parts of corn flour, 35 parts of corn husk, 7.5 parts of soybean meal, 10 parts of soybean hulls, 20 parts of bran, 3 parts of corn gluten meal, 2.5 parts of talcum powder, Mongolian 0.2 parts of de-stone and 1.8 parts of molasses.

[0029] The process of inoculating Phellinus igniarius into the solid fermentation me...

Embodiment 2

[0045] On the basis of Example 1, a preferred embodiment of the present invention is a preparation method of a composite solid probiotic containing Phellinus igniarius:

[0046] The mass parts of each component in the solid-state fermentation medium are: 17 parts of corn flour, 39 parts of corn husk, 5 parts of soybean meal, 13 parts of soybean hulls, 16 parts of bran, 4.5 parts of corn gluten meal, 1 part of talcum powder, Mongolian 0.3 part of de-stone and 0.8 part of molasses.

[0047] The process of inoculating Phellinus igniarius into the solid fermentation medium for fermentation is as follows: After the solid fermentation medium is sterilized, the Phellinus igniarius seed solution is connected with an inoculum of 5% by volume, and the water content of the culture medium is adjusted to 85%. Solid-state fermentation at 25°C for 8 days; after the fermentation is completed, the resulting solid-state fermentation medium is stirred and mixed, and the mycelium is evenly dispersed i...

Embodiment 3

[0056] On the basis of Example 2, a preferred embodiment of the present invention also includes a preparation method of a composite solid probiotic containing Phellinus linteus:

[0057] The mass parts of each component in the solid fermentation medium are: 23 parts of corn flour, 31 parts of corn husk, 9 parts of soybean meal, 7 parts of soybean hulls, 24 parts of bran, 1.5 parts of corn gluten meal, 4 parts of talcum powder, Mongolian 0.1 part of stone removal and 2.8 part of molasses.

[0058] The process of inoculating Phellinus igniarius into the solid fermentation medium for fermentation is as follows: after the solid fermentation medium is sterilized, 20% of the volume of the inoculum is connected to the Phellinus igniarius seed solution, and the water content of the culture medium is adjusted to 40%. Solid-state fermentation at 35°C for 3 days; after the fermentation is completed, the resulting solid-state fermentation medium is stirred and mixed, and the mycelium is evenly...

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Abstract

The invention relates to a preparation method of a composite solid-state probiotic agent including phellinus linteus. The method comprises the following steps of inoculating phellinus linteus seed fluid to solid-state fermentation culture mediums, performing fermentation, and degrading cellulose and hemicellulose type substances for producing monosaccharide; and then sequentially adding after fermentation bacterial fluid for a plurality of times, performing fermentation to obtain fermentation products, and then drying the fermentation products so as to obtain the composite solid-state probiotic agent. According to the preparation method disclosed by the invention, stagewise solid-state fermentation is performed according to requirement differences of different probiotics for nutrient substances, so that the culture mediums can be efficiently decomposed; phellinus linteus grows through solid-state substrates to produce active polysaccharide, and besides, the cellulose and hemicellulose type substances are degraded to generate the monosaccharide; and the solid-state probiotic agent has special paste fragrance, contains fungus polysaccharide, is high in effective viable count, is high in the content of organic nitrogen, and has the efficacy of adjusting the flora in the intestinal tracts of animals, enhancing immunity, increasing the utilization rate of the solid-state substrates, reducing the fetor of excrement of livestock and improving environment.

Description

Technical field [0001] The invention relates to a composite bacterial agent, in particular to a method for preparing a composite solid probiotic agent containing Phellinus igniarius. Background technique [0002] The composite solid probiotic is a kind of animal feed and additive product that has been studied more. At present, the conventional inoculum fermentation is a product prepared by single or multiple strains of one-time fermentation, and its content of beneficial microorganisms is less, and Insufficient decomposition and utilization of nutrients in the culture medium can not produce ideal results in the actual breeding process. In addition, most of the existing fermentations use yeast and lactic acid bacteria and other strains for fermentation, and the fermentation products and effects are roughly the same, and the nutrients in the feed are basically the same, which cannot bring new feeding effects. Summary of the invention [0003] The purpose of the present invention is...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/12A23K10/16
CPCA23K10/12A23K10/16A23K10/18
Inventor 李冠华刘力苑琳何炜何天锦
Owner INNER MONGOLIA UNIVERSITY
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