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Concentrated microbial agent of bacillus mucilaginosus and preparation method and application thereof

A technology for jelly-like spores and bacilli, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and applications, can solve the problems that the effect of microbial fertilizer cannot be effectively exerted and the number of effective viable bacteria is low.

Inactive Publication Date: 2019-10-25
FUJIAN SANJU BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, among the microbial fertilizers currently on the market, almost all of them are composite bacterial fertilizers with a variety of beneficial bacteria, and the number of effective viable bacteria is low, which cannot effectively exert the effect of microbial fertilizers

Method used

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  • Concentrated microbial agent of bacillus mucilaginosus and preparation method and application thereof
  • Concentrated microbial agent of bacillus mucilaginosus and preparation method and application thereof
  • Concentrated microbial agent of bacillus mucilaginosus and preparation method and application thereof

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preparation example Construction

[0026] The present invention also provides a preparation method of the concentrated bacterial agent, comprising the following steps:

[0027] (1) Inoculate the jelly-like bacillus in the activation medium to activate to obtain the activated bacteria; the activation medium includes raw materials of the following concentrations: 5-10 g / L of sucrose, 0.3-0.8 potassium dihydrogen phosphate g / L, magnesium sulfate 0.1~0.4g / L, sodium chloride 0.15~0.35g / L, calcium carbonate 0.5~1.2g / L and agar 20g / L;

[0028] (2) Inoculate the activating bacteria into the seed culture medium for expanded cultivation to obtain a seed fermentation broth; the seed culture medium includes raw materials of the following concentrations: 5-10 g / L of sucrose, 0.3-0.8 g of dipotassium hydrogen phosphate / L, magnesium sulfate 0.1~0.4g / L, sodium chloride 0.15~0.35g / L and calcium carbonate 0.5~1.2g / L;

[0029] (3) Inoculate the seed fermentation liquid into a fermentation medium for fermentation to obtain a fer...

Embodiment 1

[0039] according to figure 1 Shown flow process prepares microbial fertilizer, wherein:

[0040] The formula of activated medium is: sucrose 10g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L, sodium chloride 0.2g / L, calcium carbonate 1g / L, agar powder 20g / L, pH 7.5, Autoclave at 121°C for 30 minutes;

[0041] The expansion medium formula is: sucrose 10g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L, sodium chloride 0.2g / L, calcium carbonate 1g / L, pH 7.0~7.5, autoclaved at 121℃ Bacteria for 30 minutes; the conditions for primary seed cultivation are 30-35°C, 150-200r / min for 24-36h; the conditions for secondary seed cultivation can be in a seed fermentation tank, 30-35°C, 125-200r / min Cultivate under conditions for 24 to 48 hours;

[0042] The fermentation medium formula is: starch 5.5g / L, yeast powder 1.58g / L, soybean meal 5.76g / L, magnesium sulfate 1.4g / L, dipotassium hydrogen phosphate 2.0g / L, calcium carbonate 8.5g / L and chloride...

Embodiment 2

[0046] Except that the added inorganic nutrient is potassium dihydrogen phosphate 1.2%, and the organic nutrient is molasses powder 1.2%, other is the same as embodiment 1, and in the inorganic nutrient, N+P 2 o 5 +K 2 O ≥ 10g / L, organic matter ≥ 5g / L, effective viable bacteria ≥ 1 billion / mL.

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Abstract

The invention provides a concentrated microbial agent of bacillus mucilaginosus and a preparation method and application thereof, and relates to the technical field of preparation of bacterial fertilizers. The effective viable count of the bacillus mucilaginosus in the concentrated microbial agent is greater than or equal to 1 billion / mL; the bacillus mucilaginosus is a bacillus mucilaginosus Sanju No.01 strain, and the preservation number of the bacillus mucilaginosus Sanju No.01 strain is CGMCC No.3995. The content of the bacillus mucilaginosus in the concentrated microbial agent is far higher than that required by technical indexes of Agricultural Microbial Agents (GB20287-2006), and the use effect on crops is more remarkable; the process flow is simple, the operability is high, the effective viable count is high, the nutrients are rich, the photosynthesis efficiency of the crops is improved accordingly, the stress resistance is improved, the growth of the crops is facilitated, finally, the quality of the crops is improved, and the yield of the crops is increased.

Description

technical field [0001] The invention belongs to the technical field of bacterial fertilizer preparation, and in particular relates to a concentrated microbial agent of jelly-like bacillus and a preparation method and application thereof. Background technique [0002] Microbial fertilizer is developed according to the principles of soil microecology, plant nutrition, and the basic concept of modern "organic agriculture". It contains more than ten kinds of efficient and active beneficial microbial bacteria, which are suitable for various crops Activation of nutrients, improvement of nutrient utilization rate, and generality, breaking the inherent weakness of "specificity", "limitation" and "special fertilizer" of ordinary biological fertilizers. Microbial fertilizers are produced by the life activities of active (reproducible) microorganisms. A new type of fertilizer biological product for crops to obtain the required nutrients (fertilizer), which is a type of fertilizer in ag...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/04C12R1/07
CPCC12N1/20C05C11/00C05G3/00C05G3/80
Inventor 钱潘攀翟修彩肖勇仕黄今明刘玉珍朱春苗黄娟郭小红
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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