Concentrated microbial agent of bacillus mucilaginosus and preparation method and application thereof

A technology for jelly-like spores and bacilli, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and applications, can solve the problems that the effect of microbial fertilizer cannot be effectively exerted and the number of effective viable bacteria is low.

Inactive Publication Date: 2019-10-25
FUJIAN SANJU BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, among the microbial fertilizers currently on the market, almost all of them are composite bacterial fertilizers with a variety

Method used

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  • Concentrated microbial agent of bacillus mucilaginosus and preparation method and application thereof
  • Concentrated microbial agent of bacillus mucilaginosus and preparation method and application thereof
  • Concentrated microbial agent of bacillus mucilaginosus and preparation method and application thereof

Examples

Experimental program
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Example Embodiment

[0026] The present invention also provides a preparation method of the concentrated bacterial agent, which includes the following steps:

[0027] (1) The jelly-like bacillus is inoculated into an activation medium for activation to obtain activated bacteria; the activation medium includes the following raw materials: sucrose 5-10g / L, potassium dihydrogen phosphate 0.3-0.8 g / L, magnesium sulfate 0.1~0.4g / L, sodium chloride 0.15~0.35g / L, calcium carbonate 0.5~1.2g / L and agar 20g / L;

[0028] (2) Inoculating the activated bacteria into a seed culture medium for expansion culture to obtain a seed fermentation broth; the seed culture medium includes the following raw materials: sucrose 5-10g / L, dipotassium hydrogen phosphate 0.3-0.8g / L, magnesium sulfate 0.1~0.4g / L, sodium chloride 0.15~0.35g / L and calcium carbonate 0.5~1.2g / L;

[0029] (3) The seed fermentation broth is inoculated into a fermentation medium and fermented to obtain a fermentation broth; the fermentation medium includes t...

Example Embodiment

[0038] Example 1

[0039] according to figure 1 The process shown to prepare microbial fertilizer, in which:

[0040] The activation medium formula is: sucrose 10g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L, sodium chloride 0.2g / L, calcium carbonate 1g / L, agar powder 20g / L, pH 7.5, Autoclave at 121°C for 30min;

[0041] The expanded medium formula is: sucrose 10g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L, sodium chloride 0.2g / L, calcium carbonate 1g / L, pH 7.0~7.5, 121℃ high pressure quenching Bacteria 30min; the condition of primary seed culture is 30~35℃, cultivate 24~36h under the condition of 150~200r / min; the condition of secondary seed culture can be in the seed fermentation tank, 30~35℃, 125~200r / min Cultivate for 24~48h under conditions;

[0042] The formula of fermentation medium is: starch 5.5g / L, yeast powder 1.58g / L, soybean meal 5.76g / L, magnesium sulfate 1.4g / L, dipotassium hydrogen phosphate 2.0g / L, calcium carbonate 8....

Example Embodiment

[0045] Example 2

[0046] Except that the added inorganic nutrients are 1.2% potassium dihydrogen phosphate and the organic nutrients are 1.2% molasses powder, the others are the same as in Example 1, and the inorganic nutrients are N+P 2 O 5 +K 2 O≥10g / L, organic matter ≥5g / L, effective viable bacteria count ≥1.0 billion / mL.

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Abstract

The invention provides a concentrated microbial agent of bacillus mucilaginosus and a preparation method and application thereof, and relates to the technical field of preparation of bacterial fertilizers. The effective viable count of the bacillus mucilaginosus in the concentrated microbial agent is greater than or equal to 1 billion/mL; the bacillus mucilaginosus is a bacillus mucilaginosus Sanju No.01 strain, and the preservation number of the bacillus mucilaginosus Sanju No.01 strain is CGMCC No.3995. The content of the bacillus mucilaginosus in the concentrated microbial agent is far higher than that required by technical indexes of Agricultural Microbial Agents (GB20287-2006), and the use effect on crops is more remarkable; the process flow is simple, the operability is high, the effective viable count is high, the nutrients are rich, the photosynthesis efficiency of the crops is improved accordingly, the stress resistance is improved, the growth of the crops is facilitated, finally, the quality of the crops is improved, and the yield of the crops is increased.

Description

technical field [0001] The invention belongs to the technical field of bacterial fertilizer preparation, and in particular relates to a concentrated microbial agent of jelly-like bacillus and a preparation method and application thereof. Background technique [0002] Microbial fertilizer is developed according to the principles of soil microecology, plant nutrition, and the basic concept of modern "organic agriculture". It contains more than ten kinds of efficient and active beneficial microbial bacteria, which are suitable for various crops Activation of nutrients, improvement of nutrient utilization rate, and generality, breaking the inherent weakness of "specificity", "limitation" and "special fertilizer" of ordinary biological fertilizers. Microbial fertilizers are produced by the life activities of active (reproducible) microorganisms. A new type of fertilizer biological product for crops to obtain the required nutrients (fertilizer), which is a type of fertilizer in ag...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/04C12R1/07
CPCC12N1/20C05C11/00C05G3/00C05G3/80
Inventor 钱潘攀翟修彩肖勇仕黄今明刘玉珍朱春苗黄娟郭小红
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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