Low temperature anti-biological inoculant, solid state fermentation method and applications thereof

Abstract

The invention relates to the field of microbial fertilizer, and specifically discloses a low temperature anti-biological inoculant, a solid state fermentation method and applications thereof. The effective component of the low temperature anti-bacterial inoculant is pseudomonas VT-B5, and the effective viable count is 1.0*10<10> cfu / g or more. The solid state fermentation method comprises the following steps: (1) activating bacterial strains; (2) preparing a primary seed liquid; (3) preparing a secondary seed liquid; (4) carrying out solid state fermentation; (5) drying the fermentation product, adding a stabilizing agent and auxiliary agents, grinding, and packaging to obtain the low temperature anti-bacterial inoculant. The provided low temperature anti-bacterial inoculant has a good performance and high quality, can prominently improve the solid property, stress resistance and disease resistance of plants, and crop quality, and is capable of increasing the output. The production technology using a solid state fermentation method has the characteristics of low production cost, stableness, and environment-friendliness.

Description

technical field [0001] The invention relates to the field of microbial fertilizers, in particular to a low-temperature biocontrol agent and its solid-state fermentation method and application. Background technique [0002] Plant diseases are one of the natural disasters that affect agricultural production. There are three ways to prevent and control plant diseases: chemical control, physical control, and biological control. The three methods have their own advantages and disadvantages. Chemical control has quick effect, wide sterilization, and low cost. Long-term use will cause environmental pollution such as soil and air, and there are food safety problems. Some chemical fungicides have been banned in many developed countries and regions. Strictly limit the use; physical control is safe and environmentally friendly, but it is low in efficiency, high cost, time-consuming and labor-intensive; biological control, as an efficient, non-toxic, harmless, non-polluting, and non-res...

AI Technical Summary

Problems solved by technology

The three methods have their own advantages and disadvantages. Chemical control has quick effect, wide sterilization, and low cost. Long-term use will cause environmental pollution such as soil and air, and there are food safety problems. Some chemical fungicides have been banned in many developed countries and regions. Strictly limit the use; physical control is safe and environmentally friendly, but it is low in efficiency, high cost, time-consuming and labor-intensive; biological control, as an efficient, non-toxic, harmless, non-polluting, and non-resistance plant disease control method, not only meets people's expectations The demand for green food, and provides a guarantee for the sustainable development of agriculture

In actual application, biocontrol fungicides will be affected or restricted by many external factors, such as light, temperature, humidity, soil pH, etc., resulting in inability to play an obvious role in preventing and controlling pests and diseases

[0004] In northern China or southern my country, the temperature is low in winter, and the growth and metabolism of general microorganisms are obviously inhibited by temperature. At this moment, the bacterial agents with biological control function cannot exert the function of biological control due to the influence of temperature.