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Probiotic soil conditioner and production method thereof

A technology of soil conditioner and production method, which is applied in the field of probiotic soil conditioner and its production, can solve the problems of low concentration of bacteria agent, poor application effect, single function, etc., achieve short production cycle, strong vitality, and improve soil quality. environmental effects

Active Publication Date: 2012-11-21
张雯艳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The invention provides a probiotic soil conditioner and a production method thereof, which solves the problems of low bacterial agent concentration, single strain, single function, and poor application effect in the field of microbial fertilizer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of probiotic seed liquid for production: using beef extract peptone medium as the seed fermentation medium, inserting Bacillus megaterium, Bacillus subtilis, and Bacillus licheniformis into the seed medium for cultivation to obtain Bacillus megaterium seed liquid and subtilis The culturing time of Bacillus megaterium and Bacillus licheniformis was 12h, and the culturing time of Bacillus subtilis and Bacillus licheniformis was 10h.

[0039] Preparation and sterilization of the production medium: Add the production medium into the fermentor. The formula of the production medium is as follows. Each liter of the production medium contains: 15g of sucrose, 12g of lactose, 5g of maltose, and 5g of soluble starch. Peptone 15g, yeast extract 9g, urea 5g, NaCl 8g, K 2 SO 4 1.0g, K 2 HPO 4 1.5g, MgSO 4 ·7H 2 O 3.5g, CaCl 2 1.5g, CaCO 3 6.5g, the balance is water; pH 8.0. Sterilize at 121°C for 45 minutes, then cool to 34°C.

[0040] Inoculation and fermentation: The se...

Embodiment 2

[0043] Preparation of probiotic seed liquid for production: using beef extract peptone medium as the seed fermentation medium, inserting Bacillus megaterium, Bacillus subtilis, and Bacillus licheniformis into the seed medium for cultivation to obtain Bacillus megaterium seed liquid and subtilis The culturing time of Bacillus megaterium and Bacillus licheniformis was 8h, and that of Bacillus subtilis and Bacillus licheniformis was 6h.

[0044] Preparation and sterilization of the production medium: Add the production medium into the fermentor. The formula of the production medium is as follows. Each liter of the production medium contains: 8g of sucrose, 8g of lactose, 2g of maltose, and 3g of soluble starch. Peptone 6g, yeast extract 5g, urea 3g, NaCl4g, K 2 SO 4 0.44g, K 2 HPO 4 0.8g, MgSO 4 ·7H 2 O 2g, CaCl 2 0.8g, CaCO 3 4g, the balance is water; pH 8.2. Sterilize at 115°C for 45 minutes, then cool to 31°C.

[0045] Inoculation and fermentation: The seed solution of Bacillus...

Embodiment 3

[0048] Preparation of probiotic seed liquid for production: using beef extract peptone medium as the seed fermentation medium, inserting Bacillus megaterium, Bacillus subtilis, and Bacillus licheniformis into the seed medium for cultivation to obtain Bacillus megaterium seed liquid and subtilis The culturing time of Bacillus megaterium and Bacillus licheniformis was 10h, and the culturing time of Bacillus subtilis and Bacillus licheniformis was 8h.

[0049] Preparation and sterilization of the production medium: Add the production medium into the fermenter. The formula of the production medium is as follows. Each liter of the production medium contains: 10g of sucrose, 10g of lactose, 3g of maltose, and 3g of soluble starch. Peptone 10g, yeast extract 6g, urea 2g, NaCl 5g, K 2 SO 4 0.7g, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 2.5g, CaCl 2 1.1g, CaCO 3 5g, the balance is water; pH 8.0. Sterilize at 121°C for 30 minutes, then cool to 32°C.

[0050] Inoculation and fermentation: The seed ...

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Abstract

The invention discloses a production method of a probiotic soil conditioner. The production method comprises the steps of: (1) respectively inoculating huge bacillus, bacillus subtilis and bacillus licheniformis into a seed cultivation medium to cultivate and obtaining respective seed liquid; (2) adding the cultivation medium for production into a fermentation tank to sterilize and cool; and (3) inoculating the bacillus seed liquid into the fermentation tank to cultivate under an oxygen-leading agitating condition, inoculating the bacillus subtilis seed liquid and bacillus licheniformis seed liquid to cultivate, and obtaining the probiotic soil conditioner. The invention also discloses the probiotic soil conditioner obtained by the method. The method disclosed by the invention is scientific and reasonable, simple and feasible in production process and strong in industrialization implementation, and has an important significance to the development of agricultural production.

Description

Technical field [0001] The invention belongs to the technical field of agricultural bioengineering, and relates to agricultural microbial fertilizer or bio-organic fertilizer production technology, in particular to a probiotic-type soil conditioner and a production method thereof. Background technique [0002] In the 1950s and 1960s, the use of chemical fertilizers and pesticides made great progress in the development of agriculture in our country and also brought huge benefits to farmers. However, the large and excessive use of chemical fertilizers and pesticides has caused irreversible damage to the soil, the environment and even the ecosystem. For example, soil compaction; soil water holding capacity decreases; soil fertility is not balanced and fertility decreases; the ratio of input to output decreases. What's more serious is that these chemical substances will accumulate in animals and plants, causing toxic and harmful substances in animal and plant raw materials to exceed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C05G3/04C09K101/00C05G3/80
Inventor 王金玲吕长山隽加香
Owner 张雯艳
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