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Method for producing biodiesel by microalgae fermentation

A technology of biodiesel and fermentation products, applied in the field of energy, can solve the problems of not being able to achieve the maximum reaction speed and reaction efficiency, not being able to meet the requirements of the market, and harsh reaction conditions, so as to save the time and cost of preparation, fast, The effect of improving yield and production efficiency

Inactive Publication Date: 2015-04-08
RUIAN PULUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] The above-mentioned microalgae oil is first extracted with chloroform-methanol-water mixture, and then the chloroform is evaporated to obtain microalgae oil; finally, methanol and lipase are used to catalyze the microalgae oil obtained above to obtain biodiesel. High, but because the reaction of the enzyme is affected by various substances, the reaction conditions are relatively harsh, the maximum reaction speed and reaction efficiency cannot be achieved, the production cost is high, and the requirements of marketization cannot be met

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  • Method for producing biodiesel by microalgae fermentation

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Embodiment 1

[0037] The invention provides a method for producing biodiesel by microalgae fermentation, comprising the steps of:

[0038] (1) microalgae cultivation; the inoculation density of the microalgae is 0.4, the cultivation temperature of the microalgae is 28° C., and the cultivation illumination of the microalgae is 3500 lx;

[0039] (2) The above-mentioned cultured microalgae are fermented, and the fermenting bacteria are Trichoderma harzianum and Rhizopus, and the fermentation medium is microalgae 25g / L, sodium citrate 4g / L, o-toluidine 2.0g / L, chlorination Sodium 10g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 4g / L; the fermentation temperature is 30-32°C, and the fermentation time is 2-3 days; the inoculum size of the fermentation is 5%, The pH value of the fermentation is 5-6; the Trichoderma harzianum is Trichoderma harzianum, the preservation number is CCTCC NO.M 2010119, and the rhizopus is Rhizopus Rhizopus, the preservation number is CCTCC NO:M 209007.

...

Embodiment 2

[0043] The invention provides a method for producing biodiesel by microalgae fermentation, comprising the steps of:

[0044] (1) microalgae cultivation; the inoculation density of the microalgae is 0.5, the cultivation temperature of the microalgae is 28° C., and the cultivation illumination of the microalgae is 3500 lx;

[0045] (2) The microalgae of above-mentioned culture is fermented, and fermentation bacteria is Trichoderma harzianum and Rhizopus, and fermentation medium is, microalgae 30g / L, sodium citrate 5g / L, o-toluidine 1.5g / L, chlorination Sodium 12g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 3g / L; The fermentation temperature is 30-32°C, and the fermentation time is 2-3 days; The inoculum size of the fermentation is 10%. The pH value of the fermentation is 5-6; the Trichoderma harzianum is Trichoderma harzianum, the preservation number is CCTCC NO.M 2010119, and the rhizopus is Rhizopus Rhizopus, the preservation number is CCTCC NO: M 209007.

[004...

Embodiment 3

[0049] The invention provides a method for producing biodiesel by microalgae fermentation, comprising the steps of:

[0050] (1) Microalgae cultivation; the inoculation density of the microalgae is 0.45, the cultivation temperature of the microalgae is 28° C., and the cultivation light of the microalgae is 3500 lx.

[0051] (2) The above-mentioned cultured microalgae are fermented, and the fermenting bacteria are Trichoderma harzianum and Rhizopus, and the fermentation medium is microalgae 25g / L, sodium citrate 4g / L, o-toluidine 2g / L, sodium chloride 14g / L, potassium dihydrogen phosphate 0.8g / L, magnesium sulfate 4g / L; the fermentation temperature is 30-32°C, and the fermentation time is 2-3 days; the inoculum size of the fermentation is 8%, so The pH value of the fermentation is 5-6; the Trichoderma harzianum is Trichoderma harzianum, the preservation number is CCTCC NO.M 2010119, and the rhizopus is Rhizopus Rhizopus, the preservation number is CCTCC NO: M 209007.

[0052] ...

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Abstract

The invention belongs to the technical field of energy and relates to a method for producing biodiesel by microalgae fermentation. The method comprises the following steps of 1, microalgae culture, 2, fermentation of the cultured microalgae by trichoderma harzianum and rhizopus oryzae as zymophyte and a fermentation culture medium containing 20-30g / L of microalgae, 3-5g / L of sodium citrate, 1.5-2.5g / L of o-toluidine, 10-15g / L of sodium chloride, 0.5-1g / L of potassium dihydrogen phosphate and 3-5g / L of magnesium sulfate, 3, extraction of grease in the fermentation products, and 4, enzymic catalytic reaction of the grease. The biodiesel obtained by the microalgae fermentation has a fast oil production rate and a low production cost.

Description

technical field [0001] The invention belongs to the technical field of energy, and in particular relates to a method for producing biodiesel by fermenting microalgae. Background technique [0002] Biodiesel is a clean renewable energy, also known as "renewable fuel oil" or "biomass fuel". Fatty acid methyl esters are produced from crops, oil palm, pistachio and other oil-bearing forest fruits, animal fats, waste cooking oil, etc., and under the action of a catalyst (alkali or acid) through lipid exchange reaction with methanol. [0003] With the increasingly prominent issues of environmental protection and petrochemical resource depletion, biodiesel has become a hot spot in the research and development of new energy sources, and has attracted great attention from governments all over the world. Countries all over the world have selected suitable oil raw materials to produce biodiesel according to their national conditions. Europe and North America are rich in arable land r...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/885C12R1/845C12R1/89
CPCC12P7/649Y02E50/10
Inventor 杨钟华
Owner RUIAN PULUO BIOTECH
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