High-efficiency engineering bacteria agent and method for producing active biological organic fertilizer

A bio-organic fertilizer and engineering bacteria technology, applied in the biological field, can solve the problem of single fermentation bacteria and achieve the effects of activating the soil, increasing the quantity, and improving the yield and quality

Active Publication Date: 2015-12-23
杭州标航生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Based on the analysis of published patents, the production of bio-organic fertilizers by fermenting food waste is an ideal way of harmless and resourceful treatment of food waste. There is a market demand for food waste treatment, transform...

Method used

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  • High-efficiency engineering bacteria agent and method for producing active biological organic fertilizer
  • High-efficiency engineering bacteria agent and method for producing active biological organic fertilizer
  • High-efficiency engineering bacteria agent and method for producing active biological organic fertilizer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1 Experiment description

[0014] 1.1 Culture and preparation of bacteria

[0015] Pichia pastoris, Bacillus thuringiensis, Bacillus subtilis, Bacillus licheniformis, Mucor umbelliferum, and Rhizopus oryzae were inoculated in different culture media respectively.

[0016] Bacillus culture medium for Bacillus thuringiensis, Bacillus subtilis and Bacillus licheniformis: 20 parts of bran, 2 parts of soybean cake powder, 5 parts of rice bran, mixed with 25 parts of nutrient solution, and sterilized. The nutrient solution formula is: (NH 4 ) 2 SO 4 0.2%, MgSO 4 0.05%, KH 2 PO 4 0.3%, NaNO 3 0.4%, CaCl 2 0.05%, prepared with water.

[0017] Culture medium for Mucor umbellatus and Rhizopus oryzae: 15 parts of corn flour, 8 parts of bran, 5 parts of rice bran, 0.5 part of glucose, mixed with 25 parts of nutrient solution, and sterilized. Nutrient solution formula is MgSO 4 0.2%, K 2 HPO 4 0.3%, KH 2 PO 4 0.4%, yeast powder 0.05%, prepared with water.

[0018] Pi...

Embodiment 2

[0040] Pichia pastoris, Bacillus thuringiensis, Bacillus subtilis, Bacillus licheniformis, Mucor fungus, and Rhizopus oryzae were respectively inoculated in different culture media, and the culture medium formula was formulated according to the cultivation and preparation requirements of Example 1. . Aerobic culture to obtain different strains. The solid culture of bacillus is based on 37°C culture for 72-80h. At this time, the colonies of Bacillus thuringiensis, Bacillus licheniformis and Bacillus subtilis reach 4.78×10 9 CFU / g, 7.15×10 9 CFU / g and 1.01×10 10 CFU / g. Other strains such as Pichia pastoris, Rhizopus oryzae and Mucorella umbellatus were cultured at 30°C for 6-7 days, and the number of Pichia pastoris colonies was 1.41×10 9 CFU / g, the number of spores of Rhizopus oryzae and Mucor umbrella reached 1.04×10 10 CFU / g and 1.38×10 10 CFU / g.

[0041] Mix 3 parts of Pichia pastoris, 1 part of Mucorella umbellatus, 3 parts of Rhizopus oryzae, and 5 parts of Bacillus...

Embodiment 3

[0043] The culture preparation of bacteria is the same as in Example 1, the difference is that after the bacteria are cultured separately, the mixing ratio of the enzyme-producing bacteria, wherein 5 parts of Pichia pastoris, 3 parts of Mucor umbellatus, 1 part of Rhizopus oryzae, and 5 parts of Bacillus licheniformis Combine and mix, and then combine 50 parts of enzyme-producing bacteria, 6 parts of Bacillus thuringiensis and 10 parts of Bacillus subtilis to form a high-efficiency engineering bacterial agent. The bacteria agent was inoculated in the compost raw material composed of kitchen waste and straw at a ratio of 1%. After 2 weeks of aerobic fermentation, the germination index GI of active bio-organic fertilizer seeds reached 92.5%, and the C / N ratio was 16.5. The number reaches 105 million / g, and other indicators are in line with the indicators of organic fertilizer organic matter, pH, fecal coliform group, roundworm eggs, etc.

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Abstract

The invention relates to a high-efficiency engineering bacteria agent and a method for producing an active biological organic fertilizer and belongs to the technical field of biology. The bacteria agent consists of the following strains in parts by weight: 50 to 60 parts of enzyme producing bacteria, 6 to 10 parts of bacillus thuringiensis and 10 to 15 parts of bacillus subtilis, the enzyme producing bacteria consist of the following strains in parts by weight: 1 to 3 parts of umbrella mucor, 1 to 3 parts of rhizopus oryzae, 3 to 5 parts of bacillus licheniformis and 3 to 5 parts of Pichia pastoris. The high-efficiency engineering bacteria agent provided by the invention can realize high-efficiency conversion of harmlessly treated kitchen waste to obtain the high-efficiency active biological organic fertilizer. According to determination, the organic fertilizer satisfies relevant indicators of organic fertilizers in agricultural standard NT 525-2012 on organic fertilizers, can effectively increase the number of soil organic matters and effective microbial communities, can activate soil, can promote the absorption of plant root systems to nutrients, can improve the yield and quality of crops, and is a high-grade fertilizer for producing pollution-free and organic agricultural products.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-efficiency engineering bacterial agent for producing active bio-organic fertilizer and a method for producing active bio-organic fertilizer. Background technique [0002] The use of chemical fertilizers in the soil during planting can increase production and income to a certain extent, but long-term use of chemical fertilizers will cause many problems, such as lack of organic matter, soil compaction, reduced water and water retention capacity, and reduced soil quality. Moreover, a large amount of chemical fertilizers are applied to farmland, and the actual utilization rate is about 30%. Most of them flow into rivers and lakes with rainwater or farmland drainage, which is also one of the main factors causing water eutrophication pollution. Organic fertilizers can effectively improve soil physical and chemical conditions and biological characteristics, enhance soil w...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/16C05F11/08C05F9/04C12R1/07C12R1/125C12R1/10C12R1/785C12R1/845C12R1/84
CPCY02A40/20
Inventor 高永生朱丽云
Owner 杭州标航生物科技有限公司
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