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Method for preparing feed from common andrographis herb dregs by virtue of fermentation

A technology of Andrographis paniculata and dregs, applied in animal feed, animal feed, application, etc., can solve the problems of environmental pollution, waste of resources, etc., and achieve the effects of simple fermentation process, improved quality, and good palatability

Inactive Publication Date: 2015-07-22
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment methods of traditional Chinese medicine residues are mainly accumulation, incineration, and landfill, which not only pollutes the environment, but also causes waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The dregs of Andrographis paniculata are dried to a water content of 3%, crude fiber 20.4g / 100g, crude protein 13.6g / 100g, pulverized, and the powder particle size is 10 orders, taking by weighing 15g; bran 15g, wherein dregs: bran=1 : 1; corn flour 0.18g, ammonium chloride 0.18g, potassium dihydrogen phosphate 0.06g, be dissolved in the sterile water of 15g, the mixed solution that will make is poured in the Erlenmeyer flask and stirs, natural pH, at 121 ℃ autoclave for 20 minutes to obtain the medicine dregs culture medium.

[0038] Inoculate Bacillus subtilis on LB liquid medium, culture at 30°C, 200r / min, and cultivate for 24h to obtain first-grade seeds, then inoculate the first-grade seeds on LB liquid medium, cultivate at 30°C, 200r / min, for 24h, Obtain secondary seeds (bacteria concentration is 10 8 individual / mL).

[0039] Insert 4.5mL of secondary seeds into the medicinal dregs medium, ferment at 26°C for 48 hours, and shake the medium evenly every 12 hours;...

Embodiment 2

[0041] The dregs of Andrographis paniculata are dried to a water content of 3%, crude fiber 20.4g / 100g, crude protein 13.6g / 100g, pulverized, and the powder particle size is 10 orders, taking by weighing 15g; bran 15g, wherein dregs: bran=1 : 1; corn flour 0.18g, ammonium chloride 0.18g, potassium dihydrogen phosphate 0.06g, be dissolved in the sterile water of 15g, the mixed solution that will make is poured in the Erlenmeyer flask and stirs, natural pH, at 121 ℃ autoclave for 20 minutes to obtain the medicine dregs culture medium.

[0042] Inoculate Bacillus subtilis on LB liquid medium, culture at 30°C, 200r / min, and cultivate for 24h to obtain first-grade seeds, then inoculate the first-grade seeds on LB liquid medium, cultivate at 30°C, 200r / min, for 24h, Obtain secondary seeds (bacteria concentration is 10 8 individual / mL).

[0043] Insert 6.75mL of secondary seeds into the medicinal dregs medium, ferment at 30°C for 96 hours, and shake the medium evenly every 12 hours...

Embodiment 3

[0045] Andrographis paniculata dregs are dried to a water content of 3%, crude fiber 20.4g / 100g, crude protein 13.6g / 100g, pulverized, powder particle size is 10 orders, takes by weighing 20g; bran 10g, wherein dregs: bran=2 : 1; corn flour 0.18g, ammonium chloride 0.18g, potassium dihydrogen phosphate 0.06g, be dissolved in the sterile water of 15g, the mixed solution that will make is poured in the Erlenmeyer flask and stirs, natural pH, at 121 ℃ autoclave for 20 minutes to obtain the medicine dregs culture medium.

[0046] Inoculate Bacillus subtilis on LB liquid medium, culture at 30°C, 200r / min, and cultivate for 24h to obtain first-grade seeds, then inoculate the first-grade seeds on LB liquid medium, cultivate at 30°C, 200r / min, for 24h, Obtain secondary seeds (bacteria concentration is 10 8 individual / mL).

[0047] Insert 9.0mL of secondary seeds into the medicinal dregs medium, ferment for 72 hours at 28°C, and shake the medium evenly every 12 hours; dry the medium ...

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Abstract

The invention provides a method for preparing a feed from common andrographis herb dregs by virtue of fermentation. The method comprises the following steps: (1) pretreatment of dregs, namely drying the dregs until the moisture content is 2-5wt% and grinding until the power granularity is within the range of 2.5-10 meshes; (2) preparation of a dreg culture medium, namely mixing 50 parts of dregs with bran in the ratio of 1: 1 to 2: 1, 0.2-0.6 part of corn flour, 0.2-0.6 part of ammonium chloride and 0.1-0.3 part of monopotassium phosphate in 25-50 parts of sterile water, pouring the obtained mixed liquid into a conical flask and mixing evenly to obtain natural pH, and sterilizing at 121 DEG C under high pressure for 20 minutes; (3) dreg fermentation, namely inoculating 10-30mL of the second-level seeds of rhizopus oryzae strain or bacillus subtilis strain into each 100g of dreg culture medium, fermenting at 26-30 DEG C for 48-96 hours, and shaking the culture medium evenly every 12 hours; (4) product treatment, namely drying the culture medium obtained by fermentation, and grinding and sieving by use of a sieve of 2-4 meshes, thereby obtaining the fermented feed product. The method is simple in process, short in time, low in material consumption, advantageous for industrial pilot scale-up, and relatively high in yield.

Description

technical field [0001] The invention relates to a method for preparing feed by fermenting andrographis paniculata dregs, belonging to the technical field of biochemical industry. Background technique [0002] Andrographis paniculata, also known as spring lotus and autumn willow, is an annual herb with a length of 4-8 cm and a width of 1-2.5 cm. Medicinal plants have the functions of clearing away heat and detoxification, reducing inflammation, reducing swelling and relieving pain. Indications for cold and fever, sore throat, aphtha of the mouth and tongue, cough and cough, diarrhea and dysentery, hot stranguria, astringent pain, carbuncle and sore, snake bites. [0003] From the website of the State Food and Drug Administration in 2015, it can be found that there are 14 manufacturers involved in andrographis paniculata, of which 5 produce andrographolide and andrographolide semi-ester extracts. It is known that each production enterprise consumes about 3,000 tons of Androg...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/00
CPCY02P60/87
Inventor 霍清王玥
Owner BEIJING UNION UNIVERSITY
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