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Rhizopus oryzae TY GF1 bacterial strain and application thereof in degrading high-concentration oil in catering waste water

A technology for catering wastewater and Rhizopus oryzae, applied in fungi, biological water/sewage treatment, microorganism-based methods, etc., can solve the problems of toxicity, the efficiency of oil-degrading strains cannot be improved, and the efficiency is low.

Inactive Publication Date: 2012-07-04
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the existing oil-degrading bacteria strains can effectively degrade catering oil wastewater, they are limited to low-concentration catering oil wastewater, which reduces the scope of action of the oil-degrading strains and makes the efficiency of the oil-degrading bacteria strains unable to be improved. There are certain limitations in the application
However, high-concentration oils have certain toxicity to general oil-degrading strains, and the process of degrading high-concentration oils with mixed strains is very long and the efficiency is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] This example illustrates the screening process of Rhizopus oryzae (Rhizopus oryzae) TY GF1.

[0016] Using the tissue separation method, use a clean knife to remove the rotten tissue on the surface of the diseased root, and peel off its epidermis, then cut the diseased root into small pieces of 4-5mm, soak it in 70% vt alcohol for a few seconds, and then soak it in 0.1% wt Soak in acidic mercuric chloride aqueous solution for 2-3 minutes, then rinse with sterilized water for 3 times, each time for 2-3 minutes, and finally place it on PDA medium and cultivate it at 28°C. Pick the dominant colony on the PDA plate and carry out pure culture, numbered as TY.GF1.

[0017] Transfer the well-grown bacteria on the slant to the seed medium. Seed medium per liter: glucose 80g, ammonium chloride 2.0g, sodium dihydrogen phosphate 0.3g, potassium dihydrogen phosphate 0.3g, magnesium sulfate heptahydrate 0.25, 0.05 g of zinc sulfate heptahydrate, 10 g of sterile calcium carbonate wa...

Embodiment 2

[0019] This example illustrates the degradation rate of Rhizopus oryzae (Rhizopus oryzae) TY GF1 strain degrading 5g / L soybean oil wastewater, wherein the peptone is 20g / L.

[0020] In every liter of culture medium: soybean oil 5g, peptone 20g, sodium chloride 1.2g, magnesium sulfate heptahydrate 0.5g, potassium dihydrogen phosphate 1.2g, pH 6.0, the mycelia ball seeds that will cultivate are mixed with 3% (volume ratio ) inoculum amount was inoculated in the culture medium, cultured in aerobic conditions at a temperature of 35°C for 36 hours, the cultured fermentation mixture was filtered under reduced pressure, and the mycelium was washed with petroleum ether, and then the remaining oil in the fermentation liquid was extracted with petroleum ether, and the extracted The solution was detected under a UV spectrophotometer. After determination, the degradation rate was 95.6%.

Embodiment 3

[0022] The method of the present embodiment is identical with embodiment 2, changes used method parameter:

[0023] In every liter of culture medium: 10g of soybean oil, 50g of peptone, 1.2g of sodium chloride, 0.5g of magnesium sulfate heptahydrate, 1.2g of potassium dihydrogen phosphate, pH 6.0, the cultured mycelia seeds were mixed with 3% (volume ratio ) inoculum amount was inoculated in the culture medium, cultured in aerobic conditions at a temperature of 35°C for 36 hours, the cultured fermentation mixture was filtered under reduced pressure, and the mycelium was washed with petroleum ether, and then the remaining oil in the fermentation liquid was extracted with petroleum ether, and the extracted The solution was detected under a UV spectrophotometer. It was determined that the degradation rate was 88.25%.

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Abstract

Provided is a Rhizopus oryzae TY GF1 bacterial strain. The preservation date is December 20th in 2010 and the preservation registration number is CGMCC No.4490. The application of the Rhizopus oryzae TY GF1 bacterial strain in degrading high-concentration oil in catering waste water effectively is further provided.

Description

technical field [0001] The invention relates to a strain of Rhizopus oryzae TY GF1 and its application in degrading high-concentration oil in catering wastewater. Background technique [0002] Prior art: Catering grease wastewater refers to untreated wastewater containing high animal and vegetable oils and high organic matter concentration discharged from restaurants, canteens, hotels and other catering units. In the vast cities and towns, most of the catering grease wastewater generated by the daily operation of restaurants, canteens, hotels and other catering units is directly discharged into water bodies without normal treatment, which poses a serious threat to the environment. According to statistics, there are more than 4 million large and small catering units in my country, which discharge more than 20 million tons of catering oily wastewater every year. With the development of the economy, people's lives are constantly improving. In recent years, the catering industry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/845
Inventor 吴福安王俊方水琴陈明胜邱宁黎忠健张艳东梁垚姜星孙国霞
Owner JIANGSU UNIV OF SCI & TECH
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