A strain of Alternaria and its application
A technology of Alternaria sp. and inoculum, which is applied in the field of microorganisms, can solve the problems of difficulty in cultivating microbial strains, insignificant aroma enhancement effect, and complicated technical application, so as to shorten the natural aging time, shorten the fermentation and aging time, and reduce the inventory. effect of stress
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Embodiment 1
[0059] Embodiment 1, isolation and identification of Alternaria alternata HaoJ003CGMCC No.9852
[0060] 1. Separation of Alternaria alternata HaoJ003CGMCC No.9852
[0061] Tobacco leaves stored in the warehouse (aged) and field stage tobacco leaves were collected, and the microorganisms on the surface of the tobacco leaves were directly observed under a 40× stereoscope. Single spore culture was isolated in a sterile environment and cultured at 25°C for one week. For characteristics such as richness and sporulation, a representative single colony was selected and purified as the strain to be tested. A total of 1,000 microbial strains were isolated. The 1000 strains to be tested that are separated are configured as spraying bacterium liquid and spraying process is carried out to tobacco leaf respectively, and relative humidity is 20%, and temperature is after aging 90 days under the condition of 60 ℃ to the chemical composition in the treated tobacco leaf ( Soluble sugar, nicot...
Embodiment 2
[0069] Embodiment 2, Alternaria alternata (Alternaria alternata) HaoJ003CGMCC No.9852 bacterial agent is used for improving tobacco leaf quality
[0070] 1. Preparation of Alternaria alternata HaoJ003CGMCC No.9852 bacterial agent
[0071] Alternaria alternata (Alternaria alternata) HaoJ003CGMCC No.9852 was cultivated with PD liquid medium, and the preparation method of PD medium was as follows: wash and peel the potatoes, weigh 200g, cut into small pieces, add water and boil for 20-30min, Filter with gauze, add 20g of glucose, stir evenly, cool down slightly, then make up water to 1000mL, adjust the pH value to 5, divide into three-shaped bottles, autoclave at 121°C for about 20min, cool and store for later use.
[0072]Alternaria alternata (Alternaria alternata) HaoJ003CGMCC No.9852 was activated and cultivated with PDA medium (a solid medium obtained by adding agar to PD medium) at 25°C, and after activation, a puncher with a diameter of 8 mm was used to punch holes at the e...
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