A strain of Alternaria and its application

A technology of Alternaria sp. and inoculum, which is applied in the field of microorganisms, can solve the problems of difficulty in cultivating microbial strains, insignificant aroma enhancement effect, and complicated technical application, so as to shorten the natural aging time, shorten the fermentation and aging time, and reduce the inventory. effect of stress

Active Publication Date: 2019-12-24
郝勋 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still relatively few reports on microbial strains that can be used for rapid aroma enhancement and aging of flue-cured tobacco. At the same time, there are still problems such as insignificant aroma enhancement effect, long fermentation time, complicated technical application, and difficult cultivation of microbial strains, which are far from satisfying. To meet the actual needs of the development of the cigarette industry, it is necessary to seek a method that can significantly improve the quality of tobacco leaves and achieve rapid aging, which is convenient for large-scale production and popularization and application. It is harmless to humans and the environment. The microbial strains are expected by those skilled in the art

Method used

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  • A strain of Alternaria and its application
  • A strain of Alternaria and its application
  • A strain of Alternaria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, isolation and identification of Alternaria alternata HaoJ003CGMCC No.9852

[0060] 1. Separation of Alternaria alternata HaoJ003CGMCC No.9852

[0061] Tobacco leaves stored in the warehouse (aged) and field stage tobacco leaves were collected, and the microorganisms on the surface of the tobacco leaves were directly observed under a 40× stereoscope. Single spore culture was isolated in a sterile environment and cultured at 25°C for one week. For characteristics such as richness and sporulation, a representative single colony was selected and purified as the strain to be tested. A total of 1,000 microbial strains were isolated. The 1000 strains to be tested that are separated are configured as spraying bacterium liquid and spraying process is carried out to tobacco leaf respectively, and relative humidity is 20%, and temperature is after aging 90 days under the condition of 60 ℃ to the chemical composition in the treated tobacco leaf ( Soluble sugar, nicot...

Embodiment 2

[0069] Embodiment 2, Alternaria alternata (Alternaria alternata) HaoJ003CGMCC No.9852 bacterial agent is used for improving tobacco leaf quality

[0070] 1. Preparation of Alternaria alternata HaoJ003CGMCC No.9852 bacterial agent

[0071] Alternaria alternata (Alternaria alternata) HaoJ003CGMCC No.9852 was cultivated with PD liquid medium, and the preparation method of PD medium was as follows: wash and peel the potatoes, weigh 200g, cut into small pieces, add water and boil for 20-30min, Filter with gauze, add 20g of glucose, stir evenly, cool down slightly, then make up water to 1000mL, adjust the pH value to 5, divide into three-shaped bottles, autoclave at 121°C for about 20min, cool and store for later use.

[0072]Alternaria alternata (Alternaria alternata) HaoJ003CGMCC No.9852 was activated and cultivated with PDA medium (a solid medium obtained by adding agar to PD medium) at 25°C, and after activation, a puncher with a diameter of 8 mm was used to punch holes at the e...

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Abstract

The invention discloses alternaria alternata and an application thereof. The alternaria alternata HaoJ003 provided by the invention is preserved in China General Microbiological Culture Collection Center, with registration number of CGMCC No. 9852. Twenty eight tobacco related aroma components are detected from tobacco leaves which are processed by the alternaria alternata HaoJ003 CGMCC No. 9852 strain, wherein fourteen aroma compounds are not detected out from a control group, and the contents of other fourteen aroma components are improved in comparison with the control group, a nicotine content is reduced by 10.26% in comparison with the control group and the contents of harmful ingredients including one benzene, four phenols and the like are significantly declined in comparison with the control group; with the application of the alternaria alternata, tobacco aroma is enhanced, and meanwhile the contents of the harmful ingredients in the tobacco leaves are reduced; and the alternaria alternata can shorten a natural aging time and improve tobacco quality.

Description

technical field [0001] The invention relates to a strain of Alternaria and its application in the technical field of microorganisms. Background technique [0002] Harmful substances such as nicotine and nitrosamines in tobacco leaves seriously affect the quality of tobacco leaves, especially the upper tobacco leaves. The upper tobacco leaves have a high degree of dry matter filling, thick leaves, heavy single leaves, high content of ether extracts, and sufficient aroma, but the content of total sugar and reducing sugar is low, and the content of nicotine and protein is high. The proportion of various chemical components in the tobacco is not coordinated, and the miscellaneous gas and irritation are heavy, which restricts the display of the inherent aroma in the tobacco leaves, seriously affects the practicability of the upper tobacco leaves, and causes huge economic losses. In addition, this kind of tobacco leaves has slow natural aging speed, long storage period, large sit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A24B3/12A24B15/20C12R1/645
Inventor 郝勋李国强
Owner 郝勋
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