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Planting method of dendrobium officinale

A Dendrobium officinale, planting method technology, applied in the agricultural field, can solve the problems of low germination rate, weak growth, easy to get root rot, etc.

Inactive Publication Date: 2018-08-21
湖南龟寿堂健康产业发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the seeds of Dendrobium candidum have no endosperm, the germination rate is extremely low under natural conditions, so the existing Dendrobium candidum is usually cultivated into seedlings by tissue culture and then transplanted, but the tissue culture seedlings obtained by the existing tissue culture method are transplanted. After planting, the growth is weak, especially prone to root rot and other diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A planting method of dendrobium candidum, comprising the following steps: (according to different species of dendrobium candidum can be changed within the proportioning range provided below)

[0020] Step 1, get the stem tip sterilization of Dendrobium officinale; Get the newborn stem tip of Dendrobium officinale (the stem tip that grows within two days), place in 75% alcohol and sterilize for 15S, then insert 0.1% mercuric chloride For 10 minutes, rinse with sterile water 3-4 times, and dry the water with sterile paper.

[0021] Step 2, callus culture: put the sterilized shoot tip into the callus culture medium for three weeks at 30° C. to obtain the callus; the formula of the callus culture medium is 1 / 2MS medium+ NAA0.1-0.2mg / L+IAA0.2-0.3mg+6-benzylaminoadenine 0.1-0.2mg / L+5-10g / L agar+25-35g / L sucrose+5-10g / L apple juice .

[0022] Step 3, the callus is inserted into the differentiation medium, and cultivated for one week at 28°C to obtain Dendrobium candidum seed...

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PUM

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Abstract

The invention discloses a planting method of dendrobium officinale. According to the invention, by adding inactivated rhizoctonia solani and alternaria tenuissima during rooting, dendrobium officinalecan induce thallus stimulus to generate stress during rooting, and during transplanting, by amplifying the thalli symbiotic with dendrobium officinale, dendrobium officinale can have high survival rate of transplanting and low infection rate, and grow vigorously, thereby increasing the yield of dendrobium officinale.

Description

technical field [0001] This patent belongs to the field of agriculture, and in particular relates to a planting method of Dendrobium candidum. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial herb of the genus Dendrobium orchidaceae, mainly distributed in Yunnan, Anhui, Guizhou, Zhejiang, Jiangxi and other places, and is a rare traditional Chinese medicine. Because of its functions of nourishing yin and clearing away heat, promoting body fluid and benefiting the stomach, nourishing the lungs and relieving cough, it ranks first among the "Nine Chinese Immortal Herbs" and was included in the 2015 edition of the Pharmacopoeia. Its main medicinal components are Dendrobium officinale polysaccharides, alkaloids, amino acids and other trace components. Modern pharmacology shows that Dendrobium officinale has the functions of anti-tumor, anti-oxidation, anti-aging, enhancing human immunity, inhibiting tumors, lowering blood sugar, and expanding bloo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 唐敬望
Owner 湖南龟寿堂健康产业发展有限公司
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