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Aspergillus flavipes strain TR32 having inhibiting function on phytopathogens and application thereof

A technology of plant pathogenic bacteria and inhibition, applied in the field of microorganisms, can solve the problems of killing non-target organisms, resistance problems, polluting the ecological environment, etc., and achieve the effect of strong inhibition and good market application prospects

Active Publication Date: 2018-12-21
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The long-term use of large quantities of chemical pesticides not only leads to resistance problems, but also causes pesticide residues, kills non-target organisms, pollutes the ecological environment, and eventually causes harm to the human body through bioaccumulation

Method used

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  • Aspergillus flavipes strain TR32 having inhibiting function on phytopathogens and application thereof
  • Aspergillus flavipes strain TR32 having inhibiting function on phytopathogens and application thereof
  • Aspergillus flavipes strain TR32 having inhibiting function on phytopathogens and application thereof

Examples

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Effect test

Embodiment 1

[0025] 1. Screening of strains

[0026] Use a puncher to punch out bacteria cakes on the plate for biocontrol fungi and indicator bacteria, with a diameter of about 6mm. Use a pick to pick out the fungus cake, with the side of the mycelium facing down, and inoculate it on a medium plate with a diameter of 9 cm. The inoculation position is 3 cm from the edge of the petri dish. One side is for biocontrol fungi, and the other side is for indicators. bacteria. The inoculated plates were placed in a constant temperature incubator at 28°C and cultured in the dark. Observe once every 24 hours, and if there is a bacteriostatic zone, it is recorded as having a biocontrol effect.

[0027] Fungal strains with biocontrol effects were screened out, and these fungi were cultured against Fusarium graminearum according to the above method. Each treatment was repeated three times, and the average value of the radius was recorded as R. The control group was treated the same except that they ...

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Abstract

The invention provides an aspergillus flavipes strain TR32 having an inhibiting function on phytopathogens and application thereof. The strain TR32 which is obtained through screening is identified and determined that the classification of the strain TR32 is named aspergillus flavipes, the aspergillus flavipes is preserved in CCTCC (China Center for Type Culture Collection), and the preservation number is CCTCC M2018504. Bacteria inhibition experiments on common phytopathogens such as fudarium graminearum, cytospora sp, physalospora piricola and alternaria alternate verify that the strain TR32obtained in the invention has a wide-spectra and efficient bacterial inhibition function on multiple phytopathogens, the strain TR32 can be prepared into a biocontrol agent for preventing and treating the multiple phytopathogens, and a good market application prospect is obtained.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an Aspergillus flavusus strain TR32 capable of inhibiting plant pathogenic bacteria and its application. Background technique [0002] The Food and Agriculture Organization of the United Nations (FAO) estimates that the loss of crop production in the world is about 40% (440-550 billion yuan) due to damage from diseases, insect pests and weeds, and the impact on trees is even more difficult to estimate. The losses recovered through the work of scientists and various technology extension personnel engaged in pest research all over the world reached 300-400 billion yuan. For a long time, chemical control has been the main means of controlling plant diseases. The long-term use of large quantities of chemical pesticides not only leads to resistance problems, but also causes pesticide residues, kills non-target organisms, pollutes the ecological environment, and eventuall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/66
CPCA01N63/30C12N1/145C12R2001/66
Inventor 黄金光孙晓梅赵彦翔杜丰玉
Owner QINGDAO AGRI UNIV
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