Aspergillus flavipes strain TR32 having inhibiting function on phytopathogens and application thereof
A technology of plant pathogenic bacteria and inhibition, applied in the field of microorganisms, can solve the problems of killing non-target organisms, resistance problems, polluting the ecological environment, etc., and achieve the effect of strong inhibition and good market application prospects
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[0025] 1. Screening of strains
[0026] Use a puncher to punch out bacteria cakes on the plate for biocontrol fungi and indicator bacteria, with a diameter of about 6mm. Use a pick to pick out the fungus cake, with the side of the mycelium facing down, and inoculate it on a medium plate with a diameter of 9 cm. The inoculation position is 3 cm from the edge of the petri dish. One side is for biocontrol fungi, and the other side is for indicators. bacteria. The inoculated plates were placed in a constant temperature incubator at 28°C and cultured in the dark. Observe once every 24 hours, and if there is a bacteriostatic zone, it is recorded as having a biocontrol effect.
[0027] Fungal strains with biocontrol effects were screened out, and these fungi were cultured against Fusarium graminearum according to the above method. Each treatment was repeated three times, and the average value of the radius was recorded as R. The control group was treated the same except that they ...
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