The invention discloses an efficient biological bacterial fertilizer for peach trees and a preparation method and application thereof. The efficient biological bacterial fertilizer for peach trees isprepared by mixing 15 parts of a microbial agent, 30 parts of an organic fertilizer, 10 parts of nitrogen fertilizer, 8 parts of phosphorus fertilizer, 15 parts of potassium fertilizer, 5 parts of a micro fertilizer, 3 parts of chitosan, 2 parts of amino acids, 2 parts of algin, 5 parts of humic acid, and 5 parts of bentonite, screening the mixture, drying, and packaging. The efficient biologicalbacterial fertilizer for peach trees is applicable to increase of peach yield, improvement of peach quality, improvement peach shelf performance, induced enhancement of peach tree disease resistance,acceleration of peach tree growth, increase of peach root microbe quantity, improvement of soil enzyme activity and other aspects. The efficient biological bacterial fertilizer for peach trees and thepreparation method and application thereof have the advantages of significant effect, greenness with no pollution, high fertilization utilization, soil improvement, disease damage control, yield increase, quality improvement, low cost, and good popularization convenience and the like.