Method for determining cutting stopping standard of monsoon leaf cast susceptible tree by using latex physiological diagnosis technology

Abstract

The invention relates to a method for determining cutting stopping standards of monsoon leaf cast infected trees by using a latex physiological diagnosis technology, and belongs to the technical field of rubber tree disease management. The method comprises the following steps: collecting a latex sample of a susceptible plant; determining physiological parameters of latex; analyzing the correlation between the monsoon leaf cast and the latex physiological parameters, and determining the physiological parameters related to the monsoon leaf cast; latex physiological diagnosis; and determining a cutting stop standard and the like. According to the method, latex of a rubber tree infected with monsoon leaf cast is used as a material, and latex physiological parameter indexes of infected plants reaching a cutting stop standard are obtained by measuring latex physiological parameters and diagnosing latex physiology, so that early warning and accurate management of diseases are realized; the method has important significance for guiding rubber tapping production of monsoon leaf cast infected trees.

Description

Technical Field

[0001] The present invention belongs to the technical field of rubber tree disease management, and particularly relates to a method for determining the tapping suspension standard of diseased trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology. Background Art

[0002] Natural rubber is an important strategic material to ensure national security and people's livelihood. Yunnan Province is the largest and best natural rubber production base in the country, and natural rubber has developed into an important local industry. Monsoon leaf fall disease is a fungal disease infected by Phytophthora. After rubber trees are infected, rubber tree defoliation, fruit drop, tapping panel canker, etc. will occur. When the disease is severe, it will lead to the suspension of tapping, a decrease in latex production, and an increase in disease control costs, posing a huge threat to the development of the rubber industry.

[0003] In the existing technologies for preventing and controlling monsoon leaf fall disease of rubber trees, disease monitoring has lagged. Traditional diagnosis of monsoon leaf fall disease mainly relies on observing the leaf fall situation. When significant leaf fall occurs, the best prevention and control opportunity has been missed, resulting in a reduction in the yield of rubber plantations. The tapping suspension decision lacks a quantitative basis. The current tapping suspension standards are mostly based on empirical judgment and lack the support of physiological indicators directly related to the degree of pathogen infection, which is likely to cause over-tapping suspension or delayed tapping suspension, thus leading to economic losses and the spread of diseases. The existing latex physiological diagnosis technology is mainly used to evaluate the tapping intensity of normal tapping rubber trees, and the tapping intensity of diseased trees infected with monsoon leaf fall disease has not been evaluated, and the tapping suspension standard has not been determined.

[0004] Therefore, how to overcome the deficiencies of the existing technologies is an urgent problem to be solved in the current technical field of rubber tree disease management. Summary of the Invention

[0005] The purpose of the present invention is to solve the deficiencies of the existing technologies and provide a method for determining the tapping suspension standard of diseased trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology. This method uses latex physiological diagnosis technology to conduct latex physiological diagnosis on diseased trees infected with monsoon leaf fall disease, judge the health status of the tree position, calculate the latex physiological parameter indicators reaching the tapping suspension standard, so as to guide the tapping production, ensure the income of rubber farmers and social stability in border areas, and ensure the supply of national natural rubber strategic materials.

[0006] To achieve the above purpose, the technical scheme adopted by the present invention is as follows:

[0007] A method for determining the tapping suspension standard of diseased trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology, comprising the following steps:

[0008] The first step is to classify the degree of disease infection of rubber trees infected with monsoon leaf fall disease, and sample and collect latex samples of diseased plants according to the variety and degree of disease infection;

[0009] Second step, measure the physiological parameters of each collected latex sample; the physiological parameters include sucrose content, inorganic phosphorus content, thiol content, and total solid content;

[0010] Third step, analyze the correlation between monsoon leaf fall disease and latex physiological parameters, and determine the physiological parameters related to monsoon leaf fall disease;

[0011] Fourth step, compare the measured value of each physiological parameter with the reference value corresponding to the latex physiological diagnosis parameter standard, and then diagnose the health status of the rubber tree at the tree position where the sample is from according to their physiological significance; if the diagnosed result shows that the rubber tree is healthy, there is no need to stop tapping; if the diagnosed result shows that there is a problem with the health status of the rubber tree, tapping must be stopped immediately;

[0012] Among them, the value of the physiological parameter that is extremely significantly correlated with monsoon leaf fall disease for the rubber tree with the lowest disease level among the stopped-tapping trees is used as the tapping stop standard index.

[0013] Further, preferably, in the first step, the disease severity is divided into levels 0 to 5 from light to heavy.

[0014] Further, preferably, in the first step, when sampling each tree, the first 3 drops are discarded, and 10 drops are taken from each tree;

[0015] Each sample is a mixed sample of 10 trees of the same disease severity of this variety;

[0016] Each sample is made with 4 replicates.

[0017] Further, preferably, the specific method for the second step is: take 1 mL of a certain latex sample to be measured and add it to a tube containing 9 mL of buffer solution, filter it with quantitative filter paper, and the filtrate is used to measure the sucrose, inorganic phosphorus, and thiol contents;

[0018] The buffer solution formula is 0.1 g / L ethylenediaminetetraacetic acid and 25 g / L trichloroacetic acid, and the solvent is water;

[0019] The remaining latex of the latex sample to be measured is used to measure the total solid content.

[0020] Further, preferably, the anthrone method is used for sucrose content measurement, the molybdenum vanadium reaction method is used for inorganic phosphorus content measurement, the DTNB (5,5'-dithiobis(2-nitrobenzoic acid)) method is used for thiol content measurement, and the weighing method is used for total solid content measurement.

[0021] Further, preferably, the specific method for the third step is:

[0022] The original data measured in the second step are statistically analyzed and the physiological parameter values are calculated using Excel 2010, and the correlation analysis method is used to analyze the correlation between monsoon leaf fall disease and latex physiological parameters; the physiological parameter that is extremely significantly correlated with the change of the disease level of monsoon leaf fall disease is found, and this physiological parameter is used as the disease level index of monsoon leaf fall disease.

[0023] Further, preferably, the specific method of the fourth step is: compare each measured physiological parameter value with the reference value corresponding to the latex physiological diagnosis parameter standard, and record the concentration interval it is in with interval symbols.

[0024] Further, preferably, the interval symbols are specifically: "--" indicates that the concentration is in the ultra-low interval, "-" indicates that the concentration is in the low interval, "=-" indicates that the concentration is in the medium-low interval, "=" indicates that the concentration is moderate, "=+" indicates that the concentration is in the medium-high interval, "+" indicates that the concentration is in the high interval, and "++" indicates that the concentration is in the ultra-high interval.

[0025] Further, preferably, the specific method of the fourth step is: after each physiological parameter value has been compared, then according to their physiological meanings, diagnose the health status of the rubber trees at the tree positions of the sample source;

[0026] Then sort the tree positions from the lowest to the highest disease level. If the diagnosis result of the rubber trees at this disease level is healthy, there is no need to stop tapping;

[0027] If there is a problem with the health status of the rubber trees in the diagnosis result, then tapping needs to be stopped. Among them, the value of the physiological parameter that is extremely significantly correlated with the monsoon leaf fall disease for the rubber tree with the lowest disease level among the stopped tapping trees is used as the tapping stop standard index.

[0028] Further, preferably, the physiological parameter that is extremely significantly correlated with the monsoon leaf fall disease is thiol. After the antioxidant capacity of RRIM 600 deteriorates after the 3rd level, the 3rd level is the lowest tapping stop standard level, and the RSH content at the 3rd level is 0.26 mM, and this value is the tapping stop standard index;

[0029] The physiological parameter that is extremely significantly correlated with the monsoon leaf fall disease is thiol. After the antioxidant capacity of GT1 deteriorates after the 4th level, the 4th level is the lowest tapping stop standard level, and the RSH content at the 4th level is 0.35 mM, and this value is the tapping stop standard index.

[0030] In the first step of the present invention, the disease severity is divided into levels 0 to 5 from light to heavy; specifically:

[0031] Level 0: All the leaves of the whole plant are healthy;

[0032] Level 1: A small number of leaves (greater than or equal to 1 / 30 and less than 1 / 20) have a small number of disease spots;

[0033] Level 2: Most leaves (greater than or equal to 1 / 20 and less than 1 / 10) have many lesions;

[0034] Level 3: The leaves are covered with lesions, slightly wrinkled, or the leaves fall due to the disease, which is greater than or equal to 1 / 10 and less than 1 / 3;

[0035] Level 4: The leaves are severely wrinkled, or more than 1 / 3 and less than 1 / 2 of the leaves fall due to disease;

[0036] Level 5: More than 1 / 2 of the leaves fall due to disease.

[0037] In the present invention, the disease susceptibility is divided according to the severity of the lesions on the leaves of the monsoon leaf drop disease, and the health status of the rubber tree is determined by four physiological indicators - sucrose, inorganic phosphorus, thiol, and solid content.

[0038] In the present invention, the tree position refers to the rubber tapping area or number of trees that a rubber worker should complete in one working day, usually about 300 trees.

[0039] In the third step of the present invention, the correlation analysis method preferably adopts the Pearson correlation analysis method; significant correlation includes positive correlation and negative correlation.

[0040] In the fourth step of the present invention, the reference value corresponding to the standard of latex physiological diagnosis parameters is the prior art, specifically refer to: Xiao Zaiyun, Xiao Xianzhou. Research and application of physiological diagnosis of latex of Hevea brasiliensis [J]. Tropical Agricultural Science and Technology, 2009, 32(2): 46-50. Xiao Zaiyun, Liu Zhongliang, Ning Lianyun, et al. Establishment and application of reference value of physiological diagnosis standard of latex of rubber tree clone GT1 in Jiangcheng area of ​​Yunnan [C]. 2018 National Natural Rubber Academic Annual Conference of China Tropical Crops Society. China Tropical Crops Society, 2018.

[0041] In the fourth step of the present invention, the health status of the rubber tree from which the samples are derived is diagnosed according to the physiological significance they represent, which can be done with reference to the following literature: Xiao Zaiyun. Physiological microdiagnosis determination method of rubber tree latex [J]. Tropical Agricultural Science, 2005, (6): 29-31.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] (1) The standard for stopping harvesting of rubber trees infected with monsoon leaf drop disease is quantified, which can accurately stop harvesting and avoid economic losses caused by premature harvesting. The existing technology mainly observes the morphology of the leaves of diseased plants, and usually requires stopping harvesting once infection is confirmed, which is prone to premature harvesting and causing yield losses; the use of latex physiological diagnosis technology can accurately judge the health status of rubber trees and guide the timely stop of harvesting.

[0044] (2)Quantify the control indicators to prevent the large - scale spread of diseases caused by late prevention and control. The prior art does not propose the latest prevention and control red line. Due to reasons such as climate change, when the disease level is low, if no prevention and control measures are taken, the disease can sometimes be controlled, which easily leads to a fluke mentality among rubber tappers. Through latex physiological diagnosis, it is proposed that the latest prevention and control should not be later than the previous disease level before the antioxidant capacity of rubber trees deteriorates, providing rubber tappers with prevention and control standards, which can scientifically avoid large economic losses caused by late prevention and control. Specific implementation manners

[0045] The present invention will be further described in detail below in conjunction with embodiments.

[0046] Those skilled in the art will understand that the following embodiments are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. For those not specifying specific techniques or conditions in the embodiments, the techniques or conditions described in the literature in this field or according to the product instructions are followed. For those materials or equipment without indicating the manufacturer, they are all conventional products that can be obtained by purchase.

[0047] Embodiment 1

[0048] A method for determining the tapping suspension standard of diseased trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology, comprising the following steps:

[0049] First step, classify the infection degree of rubber trees infected with monsoon leaf fall disease, and sample and collect latex samples of diseased plants according to the variety and infection degree.

[0050] Second step, measure the physiological parameters of each collected latex sample; the physiological parameters include sucrose content, inorganic phosphorus content, thiol content and total solids content.

[0051] Third step, analyze the correlation between monsoon leaf fall disease and latex physiological parameters, and determine the physiological parameters related to monsoon leaf fall disease.

[0052] Fourth step, compare the measured value of each physiological parameter with the reference value corresponding to the latex physiological diagnosis parameter standard, and then diagnose the health status of the tree position where the sample is from according to their physiological significance; if the diagnosis result shows that the rubber tree is healthy, there is no need to suspend tapping; if the diagnosis result shows that there is a problem with the health status of the rubber tree, tapping should be immediately suspended.

[0053] Among them, the value of the physiological parameter that is extremely significantly correlated with monsoon leaf fall disease for the rubber tree with the lowest disease level among the trees with tapping suspended is used as the tapping suspension standard index.

[0054] Embodiment 2

[0055] A method for determining the tapping suspension standard of diseased trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology, comprising the following steps:

[0056] First step: Classify the rubber trees infected with monsoon leaf fall disease according to the degree of infection, and sample and collect latex samples from the infected plants according to the variety and the degree of infection;

[0057] Second step: Measure the physiological parameters of each collected latex sample; the physiological parameters include sucrose content, inorganic phosphorus content, thiol content, and total solid content;

[0058] Third step: Analyze the correlation between monsoon leaf fall disease and latex physiological parameters, and determine the physiological parameters related to monsoon leaf fall disease;

[0059] Fourth step: Compare the measured value of each physiological parameter with the reference value corresponding to the latex physiological diagnosis parameter standard, and then diagnose the health status of the tree position where the sample is from according to the physiological significance they represent; if the diagnosed result shows that the rubber tree is healthy, there is no need to stop tapping; if there are problems with the health status of the diagnosed rubber tree, tapping must be stopped immediately;

[0060] Among them, the value of the physiological parameter that is extremely significantly correlated with monsoon leaf fall disease for the rubber tree with the lowest disease level among the stopped-tapping trees is used as the tapping-stop standard index.

[0061] In the first step, the degree of infection is classified from light to heavy as level 0 to level 5.

[0062] In the first step, when sampling each tree, the first 3 drops are discarded, and 10 drops are taken from each tree;

[0063] Each sample is a mixed sample of 10 trees of the same variety with the same degree of infection;

[0064] Each sample is made with 4 replicates.

[0065] The specific method for the second step is: Take 1 mL of a certain latex sample to be tested and add it to a tube containing 9 mL of buffer solution, filter it with quantitative filter paper, and the filtrate is used to measure the sucrose, inorganic phosphorus, and thiol contents;

[0066] The formula of the buffer solution is 0.1 g / L ethylenediaminetetraacetic acid and 25 g / L trichloroacetic acid, and the solvent is water;

[0067] The remaining latex of the latex sample to be tested is used to measure the total solid content.

[0068] The sucrose content is measured by the anthrone method, the inorganic phosphorus content is measured by the molybdenum vanadium reaction method, the thiol content is measured by the DTNB (5,5'-dithiobis(2-nitrobenzoic acid)) method, and the total solid content is measured by the weighing method.

[0069] The specific method for the third step is:

[0070] The original data measured in the second step were statistically analyzed and the physiological parameter values were calculated using Excel 2010. The correlation analysis method was used to analyze the correlation between the monsoon leaf fall disease and the latex physiological parameters; the physiological parameter that was extremely significantly correlated with the change in the disease level of the monsoon leaf fall disease was found, and this physiological parameter was used as the disease level index for the monsoon leaf fall disease.

[0071] The specific method for the fourth step is as follows: Compare each measured physiological parameter value with the reference value corresponding to the latex physiological diagnosis parameter standard, and record the concentration range it belongs to using interval symbols.

[0072] The specific method for the fourth step is as follows: After each physiological parameter value has been compared, then according to their physiological meanings, diagnose the health status of the rubber trees at the tree positions from which the samples were taken;

[0073] Then sort the tree positions in ascending order of disease level. If the diagnosis result of the rubber trees at this disease level is healthy, there is no need to stop tapping;

[0074] If there are problems with the diagnosed health status of the rubber trees, then tapping needs to be stopped. Among them, the value of the physiological parameter that is extremely significantly correlated with the monsoon leaf fall disease for the rubber tree with the lowest disease level among the stopped-tapping trees is used as the stop-tapping standard index.

[0075] Example 3

[0076] A method for determining the stop-tapping standard for rubber trees infected with monsoon leaf fall disease using latex physiological diagnosis technology, including the following steps:

[0077] First step, classify the degree of infection of rubber trees infected with monsoon leaf fall disease, and sample and collect latex samples from infected plants according to the variety and degree of infection;

[0078] Second step, measure the physiological parameters of each collected latex sample; the physiological parameters include sucrose content, inorganic phosphorus content, thiol content, and total solids content;

[0079] Third step, analyze the correlation between the monsoon leaf fall disease and the latex physiological parameters, and determine the physiological parameters related to the monsoon leaf fall disease;

[0080] Fourth step, compare each measured physiological parameter value with the reference value corresponding to the latex physiological diagnosis parameter standard, and then according to their physiological meanings, diagnose the health status of the rubber trees at the tree positions from which the samples were taken; if the diagnosis result shows that the rubber trees are healthy, there is no need to stop tapping; if there are problems with the diagnosed health status of the rubber trees, then tapping needs to be stopped immediately;

[0081] Among them, the value of the physiological parameter that is extremely significantly correlated with the monsoon leaf fall disease for the rubber tree with the lowest disease level among the stopped-tapping trees is used as the stop-tapping standard index.

[0082] In the first step, the disease severity is divided into levels 0 to 5 from mild to severe.

[0083] In the first step, when sampling each tree, the first 3 drops are discarded, and 10 drops are taken from each tree;

[0084] Each sample is a mixed sample of 10 trees with the same disease severity of the same variety;

[0085] Each sample is replicated 4 times.

[0086] The specific method for the second step is as follows: Take 1 mL of a latex sample to be tested and add it to a tube containing 9 mL of buffer solution, filter it with quantitative filter paper, and the filtrate is used to measure the contents of sucrose, inorganic phosphorus, and thiol;

[0087] The formula of the buffer solution is 0.1 g / L ethylenediaminetetraacetic acid and 25 g / L trichloroacetic acid, and the solvent is water;

[0088] The remaining latex of the latex sample to be tested is used to measure the total solids content.

[0089] The anthrone method is used for sucrose content determination, the molybdenum vanadium reaction method is used for inorganic phosphorus content determination, the DTNB (5,5'-dithiobis(2-nitrobenzoic acid)) method is used for thiol content determination, and the weighing method is used for total solids content determination.

[0090] The specific method for the third step is as follows:

[0091] The original data measured in the second step is statistically analyzed and the physiological parameter values are calculated using Excel 2010, and the correlation analysis method is used to analyze the correlation between monsoon leaf fall disease and latex physiological parameters; find out the physiological parameters that are extremely significantly correlated with the change of monsoon leaf fall disease level, and this physiological parameter is used as the disease level index of monsoon leaf fall disease.

[0092] The specific method for the fourth step is as follows: Compare each measured physiological parameter value with the reference value corresponding to the latex physiological diagnosis parameter standard, and record the concentration range it belongs to with interval symbols.

[0093] The specific interval symbols are as follows: "--" indicates that the concentration is in the ultra-low range, "-" indicates that the concentration is in the low range, "=-" indicates that the concentration is in the low-middle range, "=" indicates that the concentration is moderate, "=+" indicates that the concentration is in the middle-high range, "+" indicates that the concentration is in the high range, and "++" indicates that the concentration is in the ultra-high range.

[0094] The specific method for the fourth step is as follows: After each physiological parameter value is compared, then according to their physiological meanings, diagnose the health status of the tree position where the sample comes from;

[0095] Then sort the tree positions from low to high according to the disease level. If the diagnosis result of the tree position at this disease level is healthy, there is no need to stop tapping;

[0096] If the diagnosis results show that there is a problem with the health of the rubber trees, harvesting must be stopped. Among them, the value of the physiological parameter of the rubber tree with the lowest disease level among the trees to be harvested and which is extremely significantly correlated with monsoon leaf drop disease shall be used as the standard indicator for stopping harvesting.

[0097] The physiological parameter that is significantly related to monsoon leaf drop is thiol. The antioxidant capacity of RRIM600 deteriorates after level 3, and level 3 is the lowest standard level for stopping harvesting. The RSH content of level 3 is 0.26mM, which is the standard indicator for stopping harvesting.

[0098] The physiological parameter that is extremely significantly correlated with monsoon leaf drop disease is thiol. The antioxidant capacity of GT1 deteriorates after level 4, and level 4 is the lowest standard level for stopping harvesting. The RSH content of level 4 is 0.35mM, which is the standard indicator for stopping harvesting.

[0099] Application Examples

[0100] Physiological parameters of latex of trees infected with GT1 and RRIM600 monsoon leaf drop disease reaching the standard of stopping harvesting

[0101] The first step was to collect latex samples of diseased plants from seven farms and one first-generation rubber plantation in Yunnan Province, including Dongfeng Farm, Mengxing Farm, Ganlanba Farm, Mengla Farm, Mengman Farm, Mengpeng Farm, Jiangcheng Farm, and Luchun Banpo Township. GT1 and RRIM600 latex produced by S / 2d3 cutting were used. Three points in each farm were selected to collect 18 samples of two varieties with different disease levels, for a total of 144 samples, and each sample was replicated 4 times. At each collection point and each disease level, 10 rubber trees with basically the same stem girth and growth were selected. The latex that flowed out 5-30 minutes after tapping was taken, 10 drops were collected from each tree, and the latex from the 10 trees was connected to a sampling tube, a total of 100 drops, which were mixed as a sample. Then 1 mL of the latex sample was drawn with a pipette and injected into a test tube containing 9 mL (0.1 g / L ethylenediaminetetraacetic acid + 25 g / L trichloroacetic acid) buffer. The sample was shaken and placed in an ice bucket together with the remaining latex sample and taken back to the laboratory for the next experiment.

[0102] The second step is the determination of physiological parameters of latex. The mixture of latex and buffer solution is filtered with medium-speed quantitative filter paper, and the filtrate is used as the sample solution for determining the content of sucrose, inorganic phosphorus and thiol. The remaining latex sample is used to determine the total solid content. The thiol content of latex is measured by the DTNB method, the sucrose content is measured by the anthrone method, and the inorganic phosphorus content is measured by the molybdenum vanadium method. The total solid content of latex is determined by weighing. Specifically, 2 mL of fresh latex is taken, put into a small sauce cup that has been weighed (W1) in advance, weighed (W2), and then placed in an oven and baked at 60°C to constant weight (W3). TSC (%) = (W3-W1) / (W2-W1)×100.

[0103] In the third step, statistical analysis (Pearson correlation analysis method) was carried out on the correlation between the physiological parameters of rubber tree latex and the disease level of monsoon leaf fall disease. The results are shown in Table 1. It can be seen that the total solid content in latex has a significant (P<0.05) negative correlation with monsoon leaf fall disease, and the correlation coefficient is -0.179; the thiol content has a highly significant (P<0.01) negative correlation with monsoon leaf fall disease, and the correlation coefficient is -0.272; although the inorganic phosphorus content increases with the increase of the disease level of monsoon leaf fall disease, the correlation with monsoon leaf fall disease does not reach a significant level (P>0.05). Therefore, the thiol content in latex was determined as the physiological parameter for judging monsoon leaf fall disease.

[0104] Table 1 Correlation analysis between monsoon leaf fall disease and latex physiological parameters

[0105] TSC SUC RSH Pi Disease level TSC 1 SUC <![CDATA[0.237 ** > 1 RSH <![CDATA[-0.195 * > <![CDATA[0.178 * > 1 Pi -0.162 -0.098 0.123 1 Disease level <![CDATA[-0.179 * > -0.094 <![CDATA[-0.272 ** > 0.048 1

[0106] Note: ** indicates highly significant correlation (P<0.01), * indicates significant correlation (P<0.05). TSC is the total solid content, SUC is the sucrose content, Pi is the inorganic phosphorus content, RSH is the thiol content, and the disease grade is the grade of monsoon leaf fall disease.

[0107] In the fourth step, the tapping suspension criteria were determined. The physiological parameters of latex at each disease level of the two varieties RRIM600 and GT1 were compared with the reference values of the physiological parameters of latex of their respective varieties. The results are shown in Table 2 and Table 3.

[0108] Table 2 Physiological diagnosis results of latex from RRIM600 plants at each disease level

[0109]

[0110] Note: In the table, ++ indicates extremely high, + indicates high, =+ indicates medium high, =-- indicates medium low, and - indicates low

[0111] As can be seen from Table 2, after the disease level of RRIM600 reaches 3, the thiol content in latex drops to medium low, indicating that the antioxidant capacity of RRIM600 deteriorates after the disease level reaches 3, and tapping should be suspended; and the thiol concentration of latex at this time, 0.26 mM, is the tapping suspension criterion for RRIM600.

[0112] Table 3 Physiological diagnosis results of latex from GT1 plants at each disease level

[0113]

[0114] Note: In the table, ++ indicates extremely high, + indicates high, =+ indicates medium high, =-- indicates medium low, and - indicates low

[0115] As can be seen from Table 3, the mercaptan content of GT1 latex decreased to medium-low after reaching Grade 4, indicating that the antioxidant capacity of GT1 plants deteriorated after reaching Grade 4 of disease infection. At this time, the mercaptan concentration of latex at 0.35 mM is the standard for stopping tapping.

[0116] Generally speaking, the mercaptan content in the latex of rubber trees can be used as a characteristic index for the prevention and control of monsoon leaf fall disease. Considering the characteristics of rapid infection and fast disease development of monsoon leaf fall disease, it is recommended that the monsoon leaf fall disease of rubber trees should be prevented and controlled as early as possible. For RRIM600, it should not be later than when the disease level reaches Grade 2, and for GT1, it should not be later than when the disease level reaches Grade 3; RRIM600 should stop tapping when the disease infection reaches Grade 3, and GT1 should stop tapping when the disease infection reaches Grade 4.

[0117] From the above examples, it can be seen that by using the latex physiological diagnosis technology, it can be accurately judged that RRIM600 should stop tapping when the disease infection reaches Grade 3, and GT1 should stop tapping when the disease infection reaches Grade 4, rather than the existing technology suggesting to stop tapping when the disease infection reaches Grade 1, which can avoid economic losses caused by premature tapping. And it is proposed that the monsoon leaf fall disease of rubber trees should be prevented and controlled as early as possible. The prevention and control red line is that for RRIM600, it should not be later than when the disease level reaches Grade 2, and for GT1, it should not be later than when the disease level reaches Grade 3, which can avoid the spread of the disease and cause greater economic losses.

[0118] Note: In the actual production process, the prevention and control of monsoon leaf fall disease lags behind. The existing technology mainly observes the morphology of the leaves of diseased plants and usually requires stopping tapping immediately after determining the infection, without specific tapping stop standards for different varieties of rubber trees.

[0119] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology, characterized in that, It includes the following steps: In the first step, classify the rubber trees infected with monsoon leaf fall disease according to the degree of infection, and sample and collect latex samples from the infected plants according to the variety and the degree of infection; In the second step, measure the physiological parameters of each collected latex sample; the physiological parameters include sucrose content, inorganic phosphorus content, thiol content, and total solid content; In the third step, analyze the correlation between monsoon leaf fall disease and latex physiological parameters, and determine the physiological parameters related to monsoon leaf fall disease; In the fourth step, compare the measured value of each physiological parameter with the reference value corresponding to the latex physiological diagnosis parameter standard, and then diagnose the health status of the rubber trees at the tree position where the sample is sourced according to their physiological significance; If the diagnosed result shows that the rubber trees are healthy, there is no need to stop tapping; if there are problems with the health status of the diagnosed rubber trees, tapping must be stopped immediately; Among them, the value of the physiological parameter that is extremely significantly correlated with the monsoon leaf fall disease in the rubber tree with the lowest disease level among the stopped-tapping trees is used as the tapping-stop standard index.

2. The method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology according to claim 1, characterized in that In the first step, the degree of infection is classified from mild to severe as level 0 to level 5.

3. The method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology according to claim 1, characterized in that, In the first step, when sampling each tree, the first 3 drops are discarded, and 10 drops are taken from each tree; Each sample is a mixed sample of 10 trees of the same variety with the same degree of infection; Each sample is replicated 4 times.

4. The method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology according to claim 1, characterized in that, The specific method for the second step is: take 1 mL of a certain latex sample to be measured and add it to a tube containing 9 mL of buffer solution, filter it with quantitative filter paper, and the filtrate is used to measure the sucrose, inorganic phosphorus, and thiol contents; The formula of the buffer solution is 0.1 g / L ethylenediaminetetraacetic acid and 25 g / L trichloroacetic acid, and the solvent is water; The remaining latex of the latex sample to be measured is used to measure the total solid content.

5. The method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology according to claim 4, characterized in that, The sucrose content is measured by the anthrone method, the inorganic phosphorus content is measured by the molybdenum-vanadium reaction method, the thiol content is measured by the DTNB (5,5'-dithiobis(2-nitrobenzoic acid)) method, and the total solid content is measured by the weighing method.

6. The method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology according to claim 1, characterized in that The specific method for the third step is: The original data measured in the second step is statistically analyzed and the physiological parameter values are calculated using Excel 2010, and the correlation analysis method is used to analyze the correlation between monsoon leaf fall disease and latex physiological parameters; Find out the physiological parameter that is extremely significantly correlated with the change in the disease level of monsoon leaf fall disease, and this physiological parameter is used as the disease level index of monsoon leaf fall disease.

7. The method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology according to claim 1, characterized in that, The specific method for the fourth step is: compare the measured value of each physiological parameter with the reference value corresponding to the latex physiological diagnosis parameter standard, and record the concentration range it is in with interval symbols.

8. The method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology according to claim 7, characterized in that, The interval symbols are specifically: "--” indicates that the concentration is in the ultra-low range, "-” indicates that the concentration is in the low range, "=-” indicates that the concentration is in the medium-low range, "=” indicates that the concentration is moderate, "=+” indicates that the concentration is in the medium-high range, "+” indicates that the concentration is in the high range, and "++” indicates that the concentration is in the ultra-high range.

9. The method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology according to claim 1, characterized in that, The specific method for the fourth step is: after each physiological parameter value has been compared, then diagnose the health status of the rubber trees at the tree position where the sample is sourced according to their physiological significance; Then sort the tree positions from the lowest to the highest disease level. If the diagnosed result of the rubber trees at the tree position of this disease level is healthy, there is no need to stop tapping; If there are problems with the health status of the rubber trees in the diagnosis result, tapping should be stopped. Among them, the value of the physiological parameter that is extremely significantly correlated with the lowest disease level of the stopped tapping trees and monsoon leaf fall disease is used as the tapping stop standard index.

10. The method for determining the tapping suspension standard of trees infected with monsoon leaf fall disease by using latex physiological diagnosis technology according to claim 9, characterized in that, The physiological parameter that is extremely significantly correlated with monsoon leaf fall disease is thiol. After the 3rd grade, the antioxidant capacity of RRIM600 deteriorates. Therefore, the 3rd grade is the lowest tapping stop standard level. The RSH content at the 3rd grade is 0.26 mM, and this value is the tapping stop standard index. The physiological parameter that is extremely significantly correlated with monsoon leaf fall disease is thiol. After the 4th grade, the antioxidant capacity of GT1 deteriorates. Therefore, the 4th grade is the lowest tapping stop standard level. The RSH content at the 4th grade is 0.35 mM, and this value is the tapping stop standard index.

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