Yield increasing method of Alternaria tenuissima activator protein

A technology of Alternaria tennatus and activating protein, which is applied in the field of β-sitosterol and betaine, can solve the problems of unfavorable fermentation and cultivation, and achieve the effect of cheap price, convenient and safe source, and convenient large-scale production

Active Publication Date: 2016-11-09
ZHEJIANG LONGYOU DONGFANG ANASAKE CROP TECHCO
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Problems solved by technology

The cultivated seeds tend to form larger clumps, which

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  • Yield increasing method of Alternaria tenuissima activator protein

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Embodiment 1

[0016] One, embodiment one. Specifically include the following steps:

[0017] (1) Preparation of spores: In an aseptic operating table, under flame protection, use an inoculation needle to spread the mycelia of Alternaria superfineum preserved in a cryopreservation tube at -90 to -70°C on a PSA medium plate, Put it into a petri dish and seal the edge of the petri dish with a parafilm, and culture it upside down in an incubator at a constant temperature of 20-35°C until it turns black and produces spores.

[0018] (2) Preparation of spore suspension: In a sterile operating table, use a 6mm puncher to punch out mycelium pieces on the PSA medium, and pour 5 mL of sterilized 0.05% Tween-20 distilled water evenly On the surface of the mycelium block, use a sterilized glass coating rod to gently sweep the surface of the colony, separate the spores from the mycelia and suspend them in sterile water, then filter the bacterial suspension with two layers of gauze to obtain a spore sus...

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Abstract

The invention relates to a yield increasing method of Alternaria tenuissima activator protein. According to the method, spores are prepared, a spore suspension liquid is prepared, and beta-sitosterol and betaine are added to a fermentation culture medium when a strain liquid is cultured, so that the yield of Alternaria tenuissima activator protein is increased. With the adoption of the yield increasing method, the culture medium and the culture conditions are optimized, so that the activator protein is increased by four times or more; ingredients of the culture medium all come from plants, the sources are convenient and safe, the cost is low, and large-scale production is facilitated; dynamic parameters of fermentation in a shaking flask are researched, and a fermentation culture medium suitable for strains to grow is acquired; influences of the inoculation amount, the initial pH value of the fermentation culture medium, the quantity of liquids in the shaking flask, the temperature and the like on fermentation are researched, and the composition of Alternaria tenuissima activator protein culture medium and the optimum fermentation culture conditions in the shaking flask are determined.

Description

technical field [0001] The invention relates to a method for increasing the production of activating protein of Alternaria tennensis, in particular to a method for increasing the production of activating protein by adding β-sitosterol and betaine to the culture medium of Alternaria tennatus. Background technique [0002] As an important member of natural and agricultural ecosystems, Alternaria tenuissima can cause plant diseases and be used by humans as useful biological resources. It has broad prospects for use in biological control, biological herbicides, environmental waste treatment, and variety breeding. Studies have shown that Alternaria tenoides has a strong degradation effect on environmental pollutants, can produce acid protease and β-l,3 glucanase, decompose vulcanized rubber and can survive on synthetic fibers; in addition, it can also produce A variety of protein elicitors with the function of disease resistance and yield increase [Qiu Dewen, Research Progress i...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C07K14/37G01N33/68C12R1/645
CPCC07K14/37C12N1/14C12N3/00G01N33/68G01N2333/37
Inventor 龚国华徐迪凡宋会鸣黄晓华
Owner ZHEJIANG LONGYOU DONGFANG ANASAKE CROP TECHCO
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