Fungus capable of converting phlorizin into phloretin and use thereof

A technology of phloretin and phlorizin, applied in the field of fungi, can solve the problems of less phloretin distribution, many by-products, and low yield, and achieve considerable economic benefits, stable performance, and low production costs

Inactive Publication Date: 2018-07-06
天津华泰至成医药科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Phloretin is rarely distributed in nature, and mainly exists in the peels, rhizomes, and root barks of succulent plants such as apples, pears, and lychees, and it is difficult to extract and separate
At p

Method used

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  • Fungus capable of converting phlorizin into phloretin and use thereof
  • Fungus capable of converting phlorizin into phloretin and use thereof
  • Fungus capable of converting phlorizin into phloretin and use thereof

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Effect test

Embodiment 1

[0031] Embodiment 1 uses the fungi of the present invention to prepare phloretin

[0032] Using the bacterial strain isolated and purchased from the laboratory as the starting strain, the following method was used to prepare phloretin.

[0033] 1) Preparation of potato liquid medium (PDA slant medium) and potato agar slant medium (PDA slant medium):

[0034] Select 200 g of commercially available fresh potatoes, peel the potatoes, cut them into small pieces, add appropriate amount of water and boil for 30 minutes. Then filter the potato boiled liquid through gauze, and add water to 1000 mL. Weigh 20 g of glucose and dissolve it in potato boiling liquid to obtain potato liquid medium. Pack every 200ml of potato liquid culture medium into a 500ml Erlenmeyer flask. 2 Sterilize under pressure at 121 °C for 30 min, and let cool to prepare fermentation broth. Take 100 mL of potato liquid culture medium, add 2 g of agar, heat to dissolve, and distribute while hot, each 5 mL into ...

Embodiment 2

[0043] Embodiment 2 uses the fungal strain of the present invention to prepare phloretin

[0044] Using the bacterial strain purchased from the laboratory as the starting strain, the following method was used to prepare phloretin.

[0045] 1) Preparation of potato liquid medium (PDA slant medium) and potato agar slant medium (PDA slant medium):

[0046] Select 200 g of commercially available fresh potatoes, peel the potatoes, cut them into small pieces, add appropriate amount of water and boil for 30 minutes. Then filter the potato boiled liquid through gauze, and add water to 1000 mL. Weigh 20 g of glucose and dissolve it in potato boiling liquid to obtain potato liquid medium. Pack every 200ml of potato liquid culture medium into a 500ml Erlenmeyer flask. 2 Sterilize under pressure at 121 °C for 30 min, and let cool to prepare fermentation broth. Take 100 mL of potato liquid culture medium, add 2 g of agar, heat to dissolve, and distribute while hot, each 5 mL into each ...

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Abstract

The invention provides a fungus for converting phlorizin into phloretin and use thereof, the standard strain is classified and named as Alternaria longipes, and is purchased from the Institute of Microbiology, Chinese Academy of Sciences, China. The preservation number is AS3.2875. The invention also provides a method of preparing the phloretin based on the fungus for converting. The fungus provided by the present invention has high yield and no environmental pollution compared with a conventional conversion method.

Description

technical field [0001] The invention belongs to the technical field of microbial transformation, and relates to a fungus for converting phloridzin into phloretin and its application. Background technique [0002] It is known that phloridizin is mainly used in the treatment of diabetes, and that phloretin is obtained by deglycosidation thereof. Phloretin is a natural dihydrochalcone substance present in apples, pears and other fruits and various vegetables. It is named for its concentrated content in the rhizomes and root barks of these plants. Phloretin has a wider range of biological activities and pharmacological effects than phlorizin: lowering blood sugar, scavenging oxygen free radicals, whitening, cardiovascular protection, anti-tumor, anti-inflammatory, immunosuppressive, weak estrogen-like activity, etc. Therefore, it is widely used in health care products, pharmaceuticals, cosmetics and other industries, and has received extensive attention from scholars at home an...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/26C12R1/645
CPCC12N1/14C12P7/26
Inventor 刘成航贾秀娟张晨曦
Owner 天津华泰至成医药科技发展有限公司
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