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Alternaria tenuissima and application of alternaria tenuissima to growth promotion and drought resistance of dendrobium officinale

A technology for extremely fine Alternaria and Dendrobium candidum, applied in the field of microorganisms, can solve the problems of artificial planting, such as slow growth rate, low average yield, occurrence of diseases, etc., and achieves good development and application prospects, easy industrial production, and simple cultivation conditions.

Active Publication Date: 2015-12-09
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

Because orchid plants and fungi have a natural symbiotic relationship, the participation of fungi is not required from the germination of its seeds to the healthy growth of seedlings, which makes the artificial cultivation of Dendrobium officinale more difficult, and the growth rate of artificial planting is slow, the average yield is low, and disease more serious

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  • Alternaria tenuissima and application of alternaria tenuissima to growth promotion and drought resistance of dendrobium officinale
  • Alternaria tenuissima and application of alternaria tenuissima to growth promotion and drought resistance of dendrobium officinale
  • Alternaria tenuissima and application of alternaria tenuissima to growth promotion and drought resistance of dendrobium officinale

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Embodiment 1

[0021] The mycorrhizal fungus Alternaria tenuissima (Alternaritenuissima) JSNL005-1 was prepared into a solid microbial agent. The specific preparation method is as follows: use cottonseed shell fungus residue as the culture substrate, ammonium sulfate as the exogenous nitrogen source, and glucose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and heat it at 121°C and 101.3 kPa Sterilize under high pressure conditions for 20 minutes. After cooling the culture medium, inoculate the mycorrhizal fungus JSNL005-1 under aseptic conditions, and then carry out artificial culture. The specific culture conditions are 26° C., 12 hours of light + 12 hours of darkness, pH 6.0, and cultured for 20 days. Process the cultured bacterial agent into 1cm 3 Blocks of the same size are placed on the surface of the Dendrobium officinale seedling cultivation substrate planted in that year. Cultivate Dendrobium candidum is selected the nutrient bo...

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Abstract

The invention discloses alternaria tenuissima, which is classified and named as alternaria tenuissima of deuteromycotina alternaria with a bacterial strain number of JSNL005-1, and is already preserved in the general microbiology center of China microbe preservation management committee with a preservation number of CGMCC No. 11311 and a preservation date of September 11, 2015. The alternaria tenuissima JSNL 005-1 disclosed by the invention has remarkable effects in aspects of improving growth rate and drought resistance ability of the dendrobium officinale, and the artificial culture can be realized; the reproductive speed is fast; the alternaria tenuissima is convenient for large-scale production, and is good in development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an Alternaria microbe and its application in growth promotion and drought resistance of Dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of the family Dendrobium genus. It has the functions of immune regulation and anti-aging. It is an extremely rare and precious Chinese medicinal material recorded in the Chinese Pharmacopoeia. Mycorrhizae are symbionts with specific morphological structures and functions formed by mycorrhizal fungi and plant roots in soil, and are a common symbiotic phenomenon in nature. Orchids are highly dependent on mycorrhizae. Mycorrhizal fungi and plant roots form a symbiont, which is a coordinated whole physiologically and forms specific morphological characteristics during the process of co-evolution. Because orchid plants and fungi have a natural symbiotic relationship, the participat...

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00A01G1/00A01G7/06C12R1/645
Inventor 徐超贾君张红岩汪善峰郑凯赵桂华杨鹤同席刚俊史俊
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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