Streptomyces carpaticus and application thereof

A technology of carp streptomyces and anthracnose bacteria, applied in the field of microorganisms

Active Publication Date: 2019-10-25
GUANGXI ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Streptomyces has been widely used in the research of biocontrol agents, it is rarely used as a bacterial fertilizer in fruit tree cultivation

Method used

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  • Streptomyces carpaticus and application thereof
  • Streptomyces carpaticus and application thereof
  • Streptomyces carpaticus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] 1 Materials and methods

[0028] 1.1 Test materials

[0029] 1.1.1 Isolation and purification of strains

[0030] Collect the soil attached to the surface of the roots of the mangrove plant Sangria apetalum (within 5mm from the roots), weigh 2.0g of the soil sample and put it in a conical flask with 20ml of sterile water (with glass beads), and shake it up by hand; Diluted 100 times and 1000 times with sterile water successively to make a coating sample solution. Take 200 μl samples and place them in 6 different media for coating, put them in an incubator at 28°C for 20 days, observe the morphological characteristics of the colonies, and pick the colonies for purification. The purified strains were stored in 20% (v / v) glycerol and frozen at -80°C.

[0031] The plant pathogenic strains tested were Banana Anthracnose, Mango Anthracnose, Pearl Prunus Anthracnose, Botrytis, Fusarium oxysporum No. 4 and Alternaria were provided by Guangxi Academy of Agricultural Sciences....

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Abstract

The invention discloses streptomyces carpaticus with the preservation number being CCTCC NO: M2019120 and taxonomically named as Streptomyces carpaticusn T33. The test shows that the streptomyces carpaticus can inhibit plant pathogenic bacteria, and has obvious antagonistic effects on banana anthracnose, mango anthracnose, pearl plum anthracnose, botryosphaeria, banana fusarium oxysporum No. 4 andalternaria alternata. Therefore, the streptomyces carpaticus can be used for biological control, the strain and the fermentation liquor of the streptomyces carpaticus have potential value of developing plant pathogenic bacteria inhibitors and have good application prospects. In addition, research discovers that the fermentation liquor of the strain can enhance the disease resistance of banana seedlings and remarkably promote growth of banana plants, and can be used as a banana growth promoter. In summary, the streptomyces carpaticus with biological control and banana growth promoting characteristics has important significance for safe and sustainable development of the agriculture.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Streptomyces cyprinis and its application. Background technique [0002] The world's population is growing at an ever-accelerating rate, and food and environmental issues have been plaguing mankind. Chemical fungicides, herbicides and fertilizers can easily damage the ecological environment of the soil, promote drug resistance of pathogenic bacteria, and endanger human health. For food and environmental safety, there is an increasing demand for non-polluting and environmentally friendly bio-sourced pesticides. Streptomyces (Streptomyces) belongs to Actinomycetes, is a Gram-positive bacterium with high G+C content (50%-80%), and is widely distributed in nature. Streptomyces can produce a variety of active compounds, antibiotics and extracellular enzymes, and it is one of the biocontrol bacteria resources with important application value. Most Streptomyces can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P21/00C12R1/465
CPCA01N63/00C12R2001/465C12N1/205A01N63/10
Inventor 潘信利王巧贞李菲李喆黄庶识姜发军赖俊翔蒙贝君
Owner GUANGXI ACAD OF SCI
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