Streptomyces carpaticus and application thereof
A technology of carp streptomyces and anthracnose bacteria, applied in the field of microorganisms
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[0027] 1 Materials and methods
[0028] 1.1 Test materials
[0029] 1.1.1 Isolation and purification of strains
[0030] Collect the soil attached to the surface of the roots of the mangrove plant Sangria apetalum (within 5mm from the roots), weigh 2.0g of the soil sample and put it in a conical flask with 20ml of sterile water (with glass beads), and shake it up by hand; Diluted 100 times and 1000 times with sterile water successively to make a coating sample solution. Take 200 μl samples and place them in 6 different media for coating, put them in an incubator at 28°C for 20 days, observe the morphological characteristics of the colonies, and pick the colonies for purification. The purified strains were stored in 20% (v / v) glycerol and frozen at -80°C.
[0031] The plant pathogenic strains tested were Banana Anthracnose, Mango Anthracnose, Pearl Prunus Anthracnose, Botrytis, Fusarium oxysporum No. 4 and Alternaria were provided by Guangxi Academy of Agricultural Sciences....
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