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A strain antagonizing the pathogenic bacteria of jujube fruit shrinkage disease and its application

A technology of pathogenic bacteria and strains, applied in the direction of application, bacteria, fungicides, etc., can solve problems such as pesticide residues and environmental pollution

Active Publication Date: 2017-01-04
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of purpose of the present invention is to provide an antagonistic bacterial strain that can efficiently prevent and treat multiple pathogenic bacteria that cause jujube fruit shrinkage disease--Bacillus subtilis STO-45 for the technical problems existing in the prevention and treatment of jujube fruit shrinkage disease. The antagonistic bacterial strain Bacillus subtilis STO-45 is a biocontrol bacterial strain isolated and screened from the root soil of healthy jujube trees. The bacterial strain of the present invention has an efficient antagonistic effect on the pathogenic bacteria of jujube fruit shrinkage disease Alternaria tenuissima , the application of the antagonistic bacterial strain Bacillus subtilisSTO-45 of the present invention can solve the problems of pesticide residues and environmental pollution in the current method of chemical prevention and treatment of jujube fruit shrinkage disease, and the antagonistic bacterial strain of the present invention can also prevent and treat jujube fruit shrinkage caused by various pathogens Anthracnose of jujube fruit caused by glyospora anthracnose and related tree diseases caused by black sour skin fungus, apple black sour skin fungus, peach dry blight, Fusarium oxysporum, Fusarium solani, etc.

Method used

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  • A strain antagonizing the pathogenic bacteria of jujube fruit shrinkage disease and its application
  • A strain antagonizing the pathogenic bacteria of jujube fruit shrinkage disease and its application
  • A strain antagonizing the pathogenic bacteria of jujube fruit shrinkage disease and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Isolation, screening and identification of jujube fruit shrinkage pathogen antagonistic bacteria Bacillus subtilis STO-45

[0058] 1) Isolation of Bacillus subtilis STO-45, an antagonistic bacterium against Jujube fruit shrinkage pathogen

[0059] The bacterial strain of the present invention is collected from healthy jujube tree root system soil in Yigaiqi Village, Yiliqi Township, Hotan County, Hotan District, Xinjiang.

[0060]1A) Weigh 5g of healthy jujube rhizosphere soil, place it in a sterilized Erlenmeyer flask, soak the soil with 50mL of sterile water, take 1ml of the suspension after slightly shaking at room temperature for 24 hours, and dilute it sequentially by 10-fold gradient dilution method to obtain 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 、10 -9 Concentration gradient dilutions, respectively from 10 -7 、10 -8 、10 -9 Take 0.1mL of the concentration gradient suspension and apply it on the NA medium plate with a sterile ...

Embodiment 2

[0080] The antibacterial activity assay of the fermented liquid of embodiment 2 Bacillus subtilis STO-45 bacterial strain

[0081] 1. Prepare the sterile filtrate of the fermentation broth

[0082] 1A) Pick an activated bacterial lawn of Bacillus subtilis STO-45, inoculate it in a 250mL Erlenmeyer flask filled with 150mL NB medium, culture it with shaking at 28°C and 200r / min for 24h, and obtain the bacterial suspension As seed liquid, NB medium: peptone 10g, beef extract 3g, NaCl 5g, water 1000ml, pH7.0-7.2;

[0083] 1B) Transfer the seed liquid to a 250mL Erlenmeyer flask containing 100mL basal medium according to the inoculum amount of 5% (V / V), shake and ferment at 28°C and 200r / min, wherein the basal medium: glucose 20g, pancreatic Peptone 20g, NaCl 5g, KH 2 PO 4 1g, K 2 HPO 4 2g, water 1000ml, pH7.0-7.2;

[0084] 1C) centrifuging the fermentation broth after 72 hours of fermentation (13000r / min, centrifuging for 10min) to obtain a centrifuged supernatant, and then ...

Embodiment 3

[0101] Example 3 Antagonism test of Bacillus subtilis STO-45 on various diseases of jujube and other tree pathogenic fungi

[0102] 1) Pick the lawn of Bacillus subtilis STO-45 cultured on NA medium for 24 hours, put it in a 250mL Erlenmeyer flask filled with 150mL of NB medium, and culture it at a constant temperature of 200r / min at 28°C for 24h to obtain a bacterial suspension As seed liquid, NB medium: peptone 10g, beef extract 3g, NaCl 5g, water 1000ml, pH7.0-7.2;

[0103] 2) Transfer the seed solution to a 250mL Erlenmeyer flask containing 100mL basal medium at an inoculum size of 5% (V / V), shake and ferment at 28°C at 200r / min, wherein the basal medium: glucose 20g, pancreatic Peptone 20g, NaCl 5g, KH 2 PO 4 1g, K 2 HPO 4 2g, water 1000ml, pH7.0-7.2;

[0104] 3) Centrifuge the fermentation broth after 72 hours of fermentation and cultivation (13000r / min, centrifuge for 10min) to obtain the centrifuged supernatant, then take the supernatant and filter it with a 0.45 ...

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Abstract

The invention discloses a bacterial strain antagonistic to pathogenic bacteria of jujube fruit shrink disease and an application of the bacterial strain. The antagonistic bacteria provided by the invention are bacillus subtilis STO-45 of which the microbial collection number is CGMCC No.9026. The bacillus subtilis STO-45 is separated from soil at the root of a healthy jujube tree and has remarkable and efficient antagonistic effect on the pathogenic bacterial of the jujube fruit shrink disease of jujube trees; the bacillus subtilis STO-45 further is capable of effectively inhibiting the alternaria tenuissima mycelia of the pathogenic bacteria of the jujube fruit shrink disease from growing and preventing and treating plant diseases induced by the pathogenic bacteria of the jujube fruit shrink disease; besides, the bacterial strain can be applied to the aspects of preventing and treating various pathogenic bacteria of the jujube fruit shrink disease, and jujube fruit anthracnose and a plurality of other tree diseases, and the like; and as a result, the bacterial strain is high in prevention effect and good in environmental safety, and has biological prevention and control potential as well as excellent development and application prospects.

Description

technical field [0001] The invention relates to an antagonistic bacterial strain, in particular to an antagonistic bacterial strain which antagonizes multiple pathogenic fungi against jujube fruit diseases and application thereof. Belonging to the field of microorganisms, it can be used for forest trees, crop protection, and disease prevention and control. Background technique [0002] Jujube tree is one of the most representative national fruit trees in my country, especially in mountainous, sandy, alkaline, and poor areas, it occupies a special and important position in helping farmers get rid of poverty and financial self-reliance. In the 1950s, a number of diseases seriously affecting jujube production were reported one after another, among which the diseases caused by fungi accounted for more than 90%. And jujube mad disease, jujube fruit shrinkage disease and jujube fruit cracking disease are the most serious harms. Compared with the jujube madness caused by Phytopla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 梁英梅田呈明武自强马荣游崇娟
Owner BEIJING FORESTRY UNIVERSITY
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