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Biocontrol bacterium for controlling plant diseases and preparation method thereof

A technology for plant fungal diseases and plant endophytic bacteria, which is applied in the field of biocontrol bacteria for preventing and treating plant diseases and its preparation, can solve the problems of limited bacillus preparations, high resistance level, resistance problems, etc., and achieves no phytotoxicity to crops. , low cost, simple cultivation method

Inactive Publication Date: 2011-09-28
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemically synthesized fungicides have the advantages of high control efficiency and convenient application, they have the following disadvantages: The resistance problem is serious: especially the development of resistance to systemic fungicides in phytopathogenic fungi is rapid and the level of resistance is high
[0005] Bacillus subtilis is the main biocontrol strain of Bacillus currently used for plant disease control, such as Baekang, Maifengning, Wenquning, Yitiande, Root Fuxiao, etc. produced in China, which are used for rice sheath blight, Prevention and treatment of root diseases such as Panax notoginseng root rot and tobacco black shank; Serenade produced abroad TM , Souata AS, Kodiak, Subtilex, BioYield, Taegro TM and Alifine-B, etc., are used to control leaf diseases such as powdery mildew, downy mildew, blight, gray mold of vegetables, cherries, grapes, gourds and walnuts and caused by Fusarium ( Fusarium spp.), Aspergillus ( Aspergillus spp.), Alternaria ( Alternaria spp.), Rhizoctonia ( Rhizoctonia spp.) and other root diseases of beans, wheat, cotton and peanuts, but the bacillus preparations for the control of powdery mildew are very limited at home and abroad

Method used

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  • Biocontrol bacterium for controlling plant diseases and preparation method thereof
  • Biocontrol bacterium for controlling plant diseases and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Isolation and identification of CC09 strain

[0014] Fresh camphor tree stems are collected from Zijin Mountain in Nanjing, Jiangsu Province, washed with tap water, dried and cut into small pieces of about 1cm. Sequentially immerse in 75% ethanol and 40% formaldehyde solution to disinfect the surface for 3 minutes each, then wash with sterile water 3 times to remove surface contamination bacteria, then cut the stem longitudinally with sterile scissors, put the cut side down on the potato sucrose On the agar plate, 5 stem segments per plate, 5 plates in total. Cultivate at 30°C, observe the appearance of new colonies every day for a total of 7 days, pick a single colony, and purify by streaking. In addition, put 2~3 uncut stems on the same potato sucrose agar plate as a control to test whether the surface sterilization is complete. The obtained strains were preserved in a -80°C refrigerator by glycerin preservation method for future use.

[0015] Inoculate ...

Embodiment 2

[0020] Embodiment two: the preparation method of CC09 bacterial strain biocontrol agent

[0021] Pick a single colony of the activated CC09 strain and inoculate it into 4ml potato sucrose medium (15ml test tube) for culture, and cultivate it on a shaker at 37°C and 100rmp for 12h, which is the seed liquid; inoculate the seed liquid at a ratio of 0.5% (v / v) in In a 1L Erlenmeyer flask containing 400ml of potato sucrose medium, ferment at 37°C and 100rmp for 48h to obtain a fermented liquid, then add 3% (w / v) surfactant OP-10, 3% (w / v) to the fermented liquid Preservative sodium salicylate and 2% (w / v) antifreeze urea to obtain biocontrol agent for Bacillus CC09 strain. Add 3% (w / v) surfactant OP-10, 3% (w / v) preservative sodium salicylate and 2% (w / v) antifreeze urea in fermented liquid, fully After mixing, the biocontrol agent of CC09 strain is obtained.

Embodiment 3

[0022] Embodiment three: CC09 bacterial strain biocontrol agent is to the inhibitory action of phytopathogenic fungus growth

[0023] The culture described in 1ml embodiment two is mixed with the potato sucrose agar medium that 14ml has melted and then poured into a petri dish with a diameter of 9 cm to make a culture plate containing 1 / 15 of the culture, and then put it into a culture dish with a diameter of 1 / 15. 7mm various fresh phytopathogenic fungus cakes, with the fungus face down, cultured in a 30°C incubator for 48 hours, measured the diameter of the colony, and replaced the culture with the same volume of medium as a blank control, and calculated the effect of the culture on various test fungi The inhibition rate of growth was repeated three times.

[0024] It can be seen from Table 2 that the CC09 strain biocontrol agent has the strongest inhibitory effect on the growth of Alternaria and Phytophthora chestnut mycelium, which is significantly higher than the positive...

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Abstract

The invention belongs to the technical field of the biological pesticide and particularly relates to a biocontrol bacterium for controlling plant diseases and a preparation method thereof. The invention has the following main contents: (1) the separation and identification of Bacillussp.CC09 strain; (2) the preparation of Bacillussp.CC09 strain biocontrol microbial inoculum and the applications of the microbial inoculum in the control of plant diseases; and (3) the stability of the Bacillussp.CC09 biocontrol microbial inoculum. The Bacillussp.CC09 biocontrol microbial inoculum is characterized in that the anti-fungal spectrum is wide, the effect is long-lasting, harmful bacteria are difficult to generate drug resistance, the microbial inoculum is safe for human, livestock and crops and is environmentally friendly, etc. The biocontrol bacterium has obvious inhibitory activity for the growth of Fusariumgraminearum, Alternariaalternata, Rhizoctoniasolani and Phytophthoracapsici and especially obvious control effect on wheat powdery mildew and scab.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a biocontrol bacterium for preventing and treating plant diseases and a preparation method thereof. Background technique [0002] Human beings are facing many challenges in the 21st century, and one of the main problems is the problem of population and food. my country's population accounts for a quarter of the world's total population, but the arable land is only 6.8% of the world's arable land. This problem is particularly prominent in our country. Therefore, increasing food production is a strategic task related to people's livelihood in our country. However, according to the survey, the world's main food crops and economic crops are lost by 20% every year due to diseases (Oerke et al, 1994. Crop Protection and Crop Production. Elsevier, Amsterdam, 808pp). At least 10-15% of food crops in our country are lost due to diseases. If fungicides are not used at a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 刘常宏双惊雷薛雅蓉李慧
Owner NANJING UNIV
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