Construction of trichoderma harzianum engineering strain having strong wide-spectrum pathogenic fungus parasitic capability and applications of the strain

A technology of pathogenic fungi and engineering strains, applied in the field of applied microorganisms, can solve problems such as affecting the colonization of exogenous Trichoderma, restricting the application of functional Trichoderma, and solve the problem of unstable field application effect, and functional bacteria to inhibit the occurrence of diseases and inhibit the occurrence of diseases. The effect of morbidity

Active Publication Date: 2016-12-21
GENLIDUO BIO TECH CO LTD
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  • Abstract
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Problems solved by technology

Due to differences in soil and crop species, changes in soil temperature and humidity, and the presence of indigenous microorganisms sig

Method used

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  • Construction of trichoderma harzianum engineering strain having strong wide-spectrum pathogenic fungus parasitic capability and applications of the strain
  • Construction of trichoderma harzianum engineering strain having strong wide-spectrum pathogenic fungus parasitic capability and applications of the strain
  • Construction of trichoderma harzianum engineering strain having strong wide-spectrum pathogenic fungus parasitic capability and applications of the strain

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Embodiment Construction

[0048] The present invention will be further described below in conjunction with specific implementation examples. The enumerated implementation cases are only used to illustrate the implementation method of the present invention, and are not intended to limit the application scope of the present invention. Where specific implementation conditions are not indicated, it is carried out according to conventional conditions well known to those skilled in the art.

[0049] 1. Discovery of parasitic factors of parasitic pathogenic fungi

[0050] 1) According to the SQR-T037 genome annotation file, determine the reported gene nox1 (see SEQ ID NO.1 for the sequence) related to the production of ROS by combining with Trichoderma genomes T.atroviride and T.harzianum CBS 226.95; http: / / genome. jgi-psf.org / Triat1 / Triat1.home.htmL; http: / / genome.jgi.doe.gov / Triha1 / Triha1.home.htmL) After blasting, combined with bioinformatics analysis, the cDNA sequence of nox1 was determined;

[0051] 2...

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Abstract

Construction of trichoderma harzianum engineering strain having strong wide-spectrum pathogenic fungus parasitic capability and applications of the strain are disclosed. That Nox1-mediated H2O2 is an important bacteriostatic component in a process of hyperparasitism of SQR-T037 on pathogenic funguses is found for the first time by the inventor. Research shows that a muton loses capability of hyperparasitism on sclerotinia sclerotiorum, fusarium oxysporum and sclerotium rolfsii when a gene nox1 is knocked out, and when a complementary nox1-expressing muton is constructed, the hyperparasitism capability of a wild type is recovered, thus confirming that the Nox1 directly regulates and controls hyperparasitism of the SQR-T037 on pathogenic funguses. An engineering fungus overexpressing the nox1 is constructed to increase the H2O2 concentration on hypha surfaces and to significantly increase capability of hyperparasitism of botryis cinerea, alternariasp, fusarium oxysporum, sclerotinia sclerotiorum, rhizoctonia solani and sclerotium rolfsii. The strain and the applications can effectively overcome a problem that disease inhibiting effects and field application effects of functional funguses are unstable in application of microbe preparations mainly comprising trichoderma and related fertilizers.

Description

technical field [0001] The invention relates to the construction and application of a strong parasitic broad-spectrum pathogenic fungus Trichoderma harzianum engineering strain and belongs to the technical field of applied microorganisms. technical background [0002] Hyperparasitism refers to a fungus that preys on or parasitizes another fungus to obtain nutrients and satisfy its own growth and reproduction; when the prey or parasitism is a plant pathogenic fungus, it can inhibit the growth and reproduction of the pathogenic fungus to achieve biological control of plant fungi effect of the disease. The most widely studied and used fungicide is Trichoderma spp., which has strong competitive ability in the rhizosphere, because it can re-parasitize plant pathogenic fungi and reduce the diseases caused by fungi; colonize the rhizosphere of plants and activate nutrients , promote plant growth, increase economic crop yield. [0003] The registered Trichoderma preparation is mai...

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Application Information

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IPC IPC(8): C12N15/80C12N15/64C12N1/15A01N63/04A01P3/00C05F11/08
CPCA01N63/30C05F11/08C12N9/0036C12N15/64C12N15/80C12Y106/03001
Inventor 沈其荣张建黄启为李荣刘东阳
Owner GENLIDUO BIO TECH CO LTD
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