94results about How to "The detection process is simple" patented technology

Belonging to the technical field of laser detection, the invention relates to a novel laser spectroscopy induced detection system, especially to a molten steel component online detection system, which is obtained by gathering lasers on a molten steel surface and conducting analysis through induced plasma spectroscopy. The laser spectroscopy induced online molten steel component detection system includes: a concave reflecting mirror, a convex reflecting mirror, a window mirror, a casing pipe, a steel furnace, a computer, a detection point distance dynamic monitoring system, a feedback regulation control device, a laser light source, a light beam turning system, an echelle spectrometer, an integral delay detection system, a laser beam quality control system and the like, and can realize accurate detection of manganese, magnesium, sulfur, carbon and other 20 components contained in molten steel. The detection system provided in the invention can realize real-time detection of molten steel components, reduce the smelting cost, and save resources.

The invention relates to a laser-induced breakdown spectroscopy based explosive real-time remote detection method, belonging to the field of the spectrum detection technology. The method comprises the steps of: measuring different regions of a sample to be detected by a laser-induced breakdown spectroscopy based measurement system to obtain a spectrum; performing normalization processing to the spectral line intensity and the intensity ratio; taking the preprocessed characteristic spectral line intensity and ratio thereof as input variables, judging by a supervised learning method or a non-supervised learning method and determining whether the sample to be detected contains an explosive. According to the method, the detection efficiency is improved, the detection process is simplified, close and remote real-time detection can be realized simultaneously, a PLS-DA (partial least squares discriminant analysis) or K mean value sorting method is used to correctly judge the explosive when the processing elements constitute a substance similar to the explosive, the detection accuracy is effectively improved, and the method can be applied to airports, railway security checking and anti-terrorist and actual battlefields.

The invention discloses a concrete surface crack width measuring instrument and method based on a smart phone. The measuring instrument comprises the smart phone and a wooden cushion block. During working, the wooden cushion block is placed beside a crack to be detected of the concrete surface, the back portion of the smart phone is tightly close to the upper surface of the wooden cushion block, and a camera of the smart phone is aligned to the crack to be detected of the concrete surface. A crack image processing program is installed on the smart phone, and crack image information acquired by the camera on the back portion of the smart phone is processed through the crack image processing program. In the method, a crack image is acquired by starting the crack image processing program and the camera of the smart phone, displayed on a screen of the smart phone and subjected to image analysis processing, and the crack width is calculated. The measuring instrument is simple in detection process, low in cost, small in size, convenient to carry and capable of achieving high-accuracy low-cost concrete surface crack width measurement.

The invention discloses a method for testing a solar energy component and a device thereof. The invention is characterized in that: a, performance parameters of the solar energy component are measured; b, longitude and latitude of the measured place are obtained by a GPS positioning system; c, meteorological data in the NASA database is transferred according to the longitude and the latitude, so as to obtain the data of annual illumination and temperature; d, annual generated energy of the component at the measured place is calculated according to the step a and the step c; the device comprises a processor, an A/D convertor, an electronic load and a sensor group, wherein, the processor receives the signals measured by the sensor group by the A/D convertor, the electric load which is connected on the sensor is taken as an artificial load of the measured component; the device is characterized in that the device also comprises a GPS positioning module which is connected with the processor by the data interface on the processor. The invention utilities the detection data of the GPS positioning system to transfer the NASA meteorological data and realizes the prediction of annual generated energy of the measured component, thus being beneficial to the construction and maintenance detection of the power station.

The invention discloses a device and method for detecting holes of disposable gloves. The device for detecting holes of disposable gloves comprises a support, a plurality of detecting heads, a main control board and an air supplying system. Each detecting head comprises a guide shaft, a sliding sleeve, a movable spindle nose and a pressure sensor, wherein the sliding sleeve is in sliding connection with the guide shaft, the movable spindle nose is detachably connected to the guide shaft, a first air duct is formed in the guide shaft, the movable spindle nose is provided with a second air duct, the first air duct is in seal communication with the second air duct, a seal ring is arranged on the inner wall of the lower end of the sliding sleeve, a second seal ring is arranged outside the movable spindle nose, the pressure sensor is connected with the first air duct, the air supplying system is connected with the first air duct through an air pipe, the guide shaft is fixed to the support, and the air supplying system and the pressure sensor are respectively in electric connection with the main control board. The detecting process of the disposable gloves is simplified through the device for detecting holes of disposable gloves, and detecting efficiency is improved.

The invention discloses a multi-gene detection method of Listeria monocytogenes based on a quantum dot/graphene oxide nanometer platform and belongs to the technical field of gene nanometer detection. The multi-gene detection method of Listeria monocytogenes comprises the following steps of: selecting two or more than two genes of the Listeria monocytogenes as target points of genetic detection and designing two pairs or more than two pairs of genetic PCR (Polymerase Chain Reaction) amplimers according to the analysis result of a gene conserved area; amplifying two or more than two genetic special sequences at the same time according to the principle of LATE-PCR to obtain a corresponding single stranded amplification product; then, hybridizing the single stranded amplification product with a quantum dot fluorescence probe; and finally, quenching and removing the non-hybridized quantum dot probe by using graphene oxide. When the target gene does not exist, all quantum dot fluorescence probes are quenched. On the contrary, when the target gene exists, corresponding fluorescent signals are obtained. The detection method has high detection reliability to Listeria monocytogenes.

The invention discloses a lead width and lead space detection method and device for a lead in a PCB image. The lead width detection method comprises the steps that binary operation processing is performed on a target PCB image so that the binary image of the target PCB image is generated; lead width structural elements are constructed according to a preset lead width threshold; morphological opening operation processing is performed on the binary image by using the lead width structural elements so that the opening operation processing image of the target PCB image is generated; difference operation processing is performed on the opening operation processing image and the binary image so that the lead width detection image of the target PCB image is generated; and the lead width detection result of the lead in the target PCB image is determined according to the lead width detection image. The lead width and the lead space of the lead in the PCB image are detected by the method so that the detection process is simpler and more rapid.

The invention provides a detection method for detecting the false welding of an electric core tab. The method comprises the steps of 1) respectively contacting the positive and negative probes of a battery tester with weldmasks on the two sides of the positive/negative tab of an electrical core so as to obtain the test internal resistance value of the positive/negative tab of the electrical core;2) comparing the test internal resistance value of the positive/negative tab of the electrical core with a standard internal resistance value; if the test internal resistance value of the positive/negative tab of the electrical core is smaller than or equal to the standard internal resistance value, judging that the positive/negative tab of the electrical core is qualified. According to the methodfor detecting the false welding of the electric core tab, the test internal resistance value of the positive/negative tab of the electrical core is compared with the standard internal resistance value, and then whether the positive/negative tab of the electrical core is qualified or not can be judged. The detection process is simple and convenient. Meanwhile, the full inspection of electrical core tabs can be realized. Test results can be fed back in time. Meanwhile, test samples are prevented from being damaged.

The invention discloses an Echo message-based multi-hop detection implementation method, and relates to the field of BFD (Bidirectional Forwarding Detection) protocol multi-hop detection. Routability is configured between two routers; an Echo message is established by a local router and transmitted to a target router; the packaging format of the Echo message is destination Mac+source Mac+0800+IP (Internet Protocol) layer payload+UDP (User Datagram Protocol) layer payload+BFD payload, wherein a destination IP included in the IP layer payload is a destination router interface IP and a source IP is a local router interface IP; after the Echo message is received by the destination router, the destination IP in the IP layer payload is changed into the local router interface IP, and the source IP is changed into the destination router interface IP to form a reply message to be transmitted to the local router; after the reply message is received by the local router, detection is performed according to the BFD protocol regulations. Multi-hop detection is realized through the Echo message, reply establishment of destination equipment is avoided and a state machine thereof is maintained, and the detection process is simplified.

The invention relates to an SNP (Single Nucleotide Polymorphism) marker and an application of the SNP marker, in particular to an SNP marker relevant to an asthma pathogenesis risk factor, a primer pair and a reagent kit for detecting the SNP marker, and an application of the SNP marker in preparing a diagnostic reagent for the asthma pathogenesis risk factor. When the site genotype of the SNP marker rs352140 is homozygous TT, a detected sample contains an asthma protection factor. The SNP marker rs352140 provides a reliable and sensitive mode for clinically evaluating the asthma pathogenesis risk factor.

The invention discloses a water quality element concentration detection method based on an unmanned aerial vehicle spectrum, and belongs to the technical field of water quality monitoring. The methodcomprises the steps that a target area is determined, target elements are determined, and sampling points are distributed in the target area; a multi-spectral camera is carried by adopting an unmannedaerial vehicle, and the sampling points are photographed in the sky above the target area so as to obtain corresponding images; the corresponding images are subjected to software analysis so as to obtain spectral characteristic data, and the corresponding spectral parameters at the sampling points are acquired through data calculation; the spectral parameters with the highest correlation with allthe target elements in the water quality are acquired through correlation analysis; an inversion model of each target element in the water quality is determined; and the spectral parameters with thehighest correlation with the target elements are input into the target element inversion models, and the output values are the concentration contents of the corresponding target elements. According tothe method, unmanned aerial vehicle remote sensing and water quality monitoring are combined, equipment involved in the whole inversion process of the water quality elements comprises the unmanned aerial vehicle and the spectral camera, and therefore the detection process is simpler and more convenient, the operation is simple, and the accuracy is high.

The invention discloses a fatigue life testing device of a car gear shift lever shifting fork. The fatigue life testing device comprises a base, a shifting fork driving mechanism arranged on the base, rolling bearings symmetrically arranged at the two ends of the shifting fork driving mechanism, a stopping block and a resistance air cylinder. The shifting fork is arranged on the shifting fork driving mechanism. The shifting fork is connected with a sliding shaft which is supported through the rolling bearings. The resistance air cylinder is connected with an air cylinder piston rod, a force sensor and a pressing disc. The whole fatigue life testing device of the car gear shift lever shifting fork is novel in structure, a testing process is simple, testing results are reliable, and cost is low.

The invention relates to a discrimination method of flue-cured tobacco producing areas. The discrimination method comprises the following steps: establishing a discrimination model involving a positive red-green index, a positive yellow-blue index, positive and negative brightness difference, positive and negative whiteness index difference, positive and negative total color difference, a positivewhiteness index and positive color saturation of tobacco leaves and the flue-cured tobacco producing areas; detecting quantitative color indexes of tobacco leaves to be discriminated, and substituting into the discrimination model. By the discrimination method of the flue-cured tobacco producing areas, provided by the invention, the screened quantitative color indexes such as the positive red-green index, the positive yellow-blue index and the like have very strong correlativity with the flue-cured tobacco producing areas; by a modeling method, regional appearance characteristics of the tobacco leaves from different producing areas can be reflected very well, so that the flue-cured tobacco producing areas can be effectively identified by using the established discrimination model; the quantitative color indexes can be conveniently detected by using a colorimeter, the detection process is simple, and requirements on the detection environment and samples are low; the discrimination method has a very good application prospect in the process of precisely evaluating the quality of the tobacco leaves and automatically classifying and sorting the tobacco leaves.

The invention discloses a method for determining a content of hydrofluoric acid in a nitric acid-hydrofluoric acid pickling solution. The method comprises the steps of preparing a sodium citrate-sodium nitrate total ion strength buffer solution, preparing a fluorine standard storage solution, preparing a fluorine standard solution A and a fluorine standard solution B, preparing a test sample solution, preparing a fluorine standard application solution A and a fluorine standard application solution B, preparing a test sample application solution, mounting an ion meter, calibrating a slope when in concentration measurement, measuring the concentration, calculating the content of the hydrofluoric acid of a test sample and the like. By adopting the precision ion meter, a fluorine ion selective electrode is adopted as an indicator electrode which is inserted into a solution to be detected together with a reference electrode, so that an electrochemical system for determination is formed; a concentration mode is selected, and a two-point calibration is adopted; and by adding the total ion strength buffer solution, the influence of interference ions and acidity can be eliminated, and the content of the hydrofluoric acid in the test solution is directly determined. The determination method is simple, convenient, rapid and environment-friendly in the detection process, low in expense and cost, accurate and consistent in detection results, and capable of supporting the maintenance and quality control of the acid pickling solution.

The invention discloses a method for detecting acetyl choline by a fluorescence ratio. The method comprises the following steps of step1, preparing multiple standard solutions with the same volume, having the same concentrations of acetylcholin esterase, choline oxidase, horse radish peroxidase, o-phenylenediamine and carbon quantum dots, and having the different concentrations of acetyl choline;step 2, detecting the fluorescence intensities I442 and I573 of the multiple standard solutions at 442nm and 573nm, and building a linear relation between the I573/I442 and the acetyl choline concentration; and step 3, preparing a solution to be detected with the unknown acetyl choline concentration, detecting the fluorescence intensities I442 and I573 of the solution at 442nm and 573nm, and thuscomputing the acetyl choline concentration in the solution to be detected according to the linear relation acquired in the step 2. The method provided by the invention has the advantages of simple operation, quick detection, high sensitivity and the like.

A five-axis numerical control machine tool rotating shaft structure parameter error compensation method comprises the steps: under the condition that an RTCP function is started, equally dividing therotating stroke of each rotating shaft of a five-axis numerical control machine tool into a plurality of nodes, and controlling a machine tool to swing to a given node in the detection process of eachrotating shaft; collecting offsets of the cutter center point relative to the initial position under each node in the X-axis direction, the Y-axis direction and the Z-axis direction; establishing a rotating shaft structure parameter error identification mathematical model based on the tool nose point position deviation under the action of the tool center point deviation error, obtaining an identification equation, and calculating a machine tool rotating shaft structure parameter error; compensating the structure parameter error value into a rotating shaft structure parameter corresponding tothe numerical control system to realize error compensation of the machine tool rotating shaft; collecting a knife point RTCP error to verify an error compensation effect, determining whether a rotating shaft structure parameter error is eliminated or not, and if the rotating shaft structure parameter error is eliminated, ending; and if the rotating shaft structure parameter error is not eliminated, carrying out the operation again.

The invention relates to an apparatus for detecting bamboo strip edge defects. The apparatus comprises a frame, an input component, multiple positioning mechanisms for compacting and positioning bamboo strips and an output component; the input component, multiple positioning mechanisms and the output component are arranged on the upper end of the frame in a straight line in order; one side of each positioning mechanism closed to the input component is provided with a laser diffuse reflection sensor, and a spacing mechanism which contacts the bamboo strip edge is arranged between every two adjacent positioning mechanisms; one end of the each spacing mechanism near the bamboo strip is provided with a fiber amplifier. Compared with prior, the apparatus is used for positioning, spacing and transmitting, edges of bamboo strips are used for detecting defected state, in order to realize edge defect defection of bamboo strips; and the detection process is simple and has high efficiency, and the detection effects are better.

This invention can qualitative and quantitative measure the phosphate radical content in blood by use of HEPES buffer solvent with PH value 6.5 to 7.5 and with pyrocatechol violet and ytterbium ion as color-developing agent. This method is free of interference of other contents and can be used in clinic.

The invention discloses a 21 hydroxylase gene mutation detection method and a 21 hydroxylase gene mutation detection kit. According to the method and the kit, based on the human 21 hydroxylase gene mutation characteristics, the hot-spot mutation long-segment deletion is used as a detection object, a RCCX module is subjected to long-fragment PCR amplification by selecting a specific long-fragment PCR amplification primer, the long-fragment amplification product is subjected to enzymatic cleavage reaction detection, whether the detected gene has long-fragment deletion can be intuitively detectedthrough an electrophoresis map, and by combining a high-throughput sequencing technology, the point mutation, the short-fragment insertion of deletion, and other mutation information can be detected.According to the present invention, the whole detection process is simple and rapid, the detection flux is high, the specificity is strong, and the detection result is accurate and reliable.

The invention discloses an automatic detection device for a workpiece surface ultrasonic phased array. The automatic detection device comprises a crawler, a scanning guide rail, an ultrasonic phased array instrument and a motion controller, wherein the crawler is arranged on a to-be-detected workpiece and can move on the to-be-detected workpiece, one end of the crawler is arranged on the scanningguide rail, an ultrasonic phased array instrument is arranged on the scanning guide rail, and the scanning guide rail is arranged on the surface of the to-be-detected workpiece and collects ultrasonicdetection data of the surface of the to-be-detected workpiece. According to the detection device provided by the invention, the pipeline crawl device drives the ultrasonic phased array probe to automatically detect, so that the safety condition of the pipeline can be better judged, and safety quality production can be better controlled; the liquid supply module and the liquid suction module workin cooperation to provide ultrasonic detection coupling water supply, meanwhile, overflowing coupling water is sucked away and filtered into the water supply water tank, and therefore cyclic utilization of the ultrasonic detection coupling water is achieved; consumption of ultrasonic detection coupling water is greatly reduced.

The invention discloses a method for detecting concentration of horseradish peroxidase by utilizing a water-soluble InP/ZnS QDs probe. The method comprises the following steps: step 1, preparing horseradish peroxidase standard solutions which have different concentration and contain water-soluble InP/ZnS QDs; detecting the fluorescence intensity of the standard solutions to obtain fluorescence spectrograms of the standard solutions; establishing a linear relation of a difference value between the fluorescence intensity of the standard solution with the concentration of the horseradish peroxidase of zero and the fluorescence intensity of all the standard solutions and the concentration of the horseradish peroxidase; step 2, preparing a horseradish peroxidase sample solution containing the water-soluble InP/ZnS QDs and detecting the fluorescence intensity of the standard solution; and determining the concentration of the horseradish peroxidase in the sample solution through the linear solution. By taking the InP/ZnS QDs as the probe and utilizing the property of quenching the fluorescence of the water-soluble InP/ZnS QDs of the horseradish peroxidase, the concentration of the horseradish peroxidase is detected; and a detection process is simple and convenient, high in sensitivity and low in detection limit, and online and in-situ rapid and sensitive detection of the concentration of the horseradish peroxidase can be realized.

The invention discloses a dam surface crack intelligent identification and measurement device for an unmanned aerial vehicle. The device comprises a crack identification device, a crack measurement device, a crack positioning device, a storage and connection device and a comprehensive processor, wherein the crack recognition device can shoot a video along with inspection of the unmanned aerial vehicle, intelligently recognize a crack, capture a crack picture and draw the shape of the crack; the crack measuring device is used for measuring the width of a crack by utilizing a laser width gauge and generating a crack width table; and the crack positioning device is used for determining the coordinates of the crack. The intelligent crack recognition and measurement device can quickly detect the position of a dam surface crack, shoot a crack picture, measure the width of the crack and automatically generate a dam surface crack distribution diagram, so that the number of times of dam entering detection of engineers can be reduced, a large amount of manpower and time can be saved, and the crack recognition and measurement efficiency and precision can be improved.

The invention discloses a detection method for a glue residue on the hole wall of a PCB. The detection method comprises the following steps: putting a detection sample in a solution capable of carrying out a replacement reaction with a copper film on the hole wall so that the replacement reaction of copper and the solution is carried out; observing the whole circular ring of the hole wall at the position of an inner-layer circuit of the PCB; judging whether glue removal is qualified according to the color of the whole circular ring of the hole wall, wherein the detection sample is the hole of the PCB deposited with the copper film on the hole wall. According to the detection method, as long as the detection sample is put in the solution capable of carrying out the replacement solution with the copper, the color of the whole circular ring of the hole wall at the position of the inner-layer circuit of the PCB can be intuitively observed after the reaction is completed so as to judge whether the glue removal is qualified, and an expensive fluorescence detector adopted in the prior art is not needed, so that the cost for detecting the glue residue on the hole wall is reduced, and the detection method is simple in detection process and easy in operation and can be widely popularized and applied in the preparation technology of PCB.

The invention relates to an environment anomaly detection method and device, computer equipment and a storage medium. The method comprises the following steps: acquiring a to-be-detected image of a current detection period; according to the image features in the to-be-detected image, if it is judged that environment anomaly exists in the to-be-detected image, obtaining all environment anomaly detection frames in the to-be-detected image according to the image features in the to-be-detected image, wherein each environment anomaly detection frame carries position information, category and confidence, the position information is used for identifying the position information of the corresponding environment anomaly detection frame, the category is used for identifying the category of the abnormal environment information contained in the corresponding environment anomaly detection frame, and the confidence coefficient is used for identifying the confidence coefficient of the category of the abnormal environment information contained in the corresponding environment anomaly detection frame; and determining an environment anomaly detection result of the to-be-detected image according to the position information, the category and the confidence coefficient carried by each environment anomaly detection frame. By adopting the method, the detection efficiency of the environment abnormity can be improved.

The invention discloses a reagent for detecting SNP sites of P53 genes and application of the reagent. The reagent comprises a reagent which can be used for detecting genotype of rs17881013 site in the P53 genes. A patient is subjected to genetic typing, correlation between the SNP sites of P53 genes and gefitinib on treatment response of advanced non-small cell lung cancer is analyzed, and the treatment response of a patient suffering from the advanced non-small cell lung cancer on the gefitinib can be judged according to the genotype of the rs17881013 site. The development of the reagent provides a reliable and sensitive mode for determining a treatment plan of the patient suffering from the advanced non-small cell lung cancer.

The invention provides a surface roughness detecting device and an operating method thereof, and belongs to the technical field of detecting devices. The device comprises a V-shaped tray for holding asteel coil to be detected, and a surface topography detecting device comprising a moving frame, a Z-direction slide rail, a surface measuring component, a main box, a motion operation panel and a measurement operation panel. A plurality of universal wheels are disposed on the bottom surface of the moving frame. A pair of opposite positioning blocks is disposed on the bottom surface of the movingframe. The pair of positioning blocks realizes the centering of the surface topography detecting device. The moving frame is further provided with an X-direction slide rail. A slide block is arrangedon the X-direction slide rail. The Z-direction slide rail is slidingly matched with the slide block. The surface measuring component is disposed at the lower end of the Z-direction slide rail. The main box, the motion operation panel and the measurement operation panel are all disposed the moving frame. The surface roughness detecting device is suitable for the surface topography detection of rollers, steel coils and steel strips, and is convenient to use.

The invention discloses a screw verticality detection device, which comprises a base, a guide rod vertically arranged above the base, and a bearing seat sleeved outside the guide rod, and a linear bearing is arranged inside the bearing seat. The linear bearing is slidingly fitted on the outside of the guide rod, and a first locking wrench is provided on one side of the lower end of the bearing seat, and one end of the first locking wrench passes through a threaded hole to abut against the guide rod, and the One side of the upper end of the bearing seat is provided with a dial indicator and a second locking wrench, the measuring end of the dial indicator is perpendicular to the guide rod, and one end of the second locking wrench passes through the threaded hole to abut against the dial indicator. The guide rod is used as the measuring track of the dial indicator, which ensures the stability of the measurement data. The first locking wrench is used for fixing the bearing seat, and the second locking wrench is used for the dial indicator. Subtable fixation. The invention has the advantages of convenient use, simple operation, high detection precision and low investment cost.

An inspection device for an excircle boss height difference of a thin-wall rotation-type part comprises a base plate. Two stand columns are arranged on the base plate, a transverse rod parallel to the base plate is arranged between upper ends of the two stand columns, a fixing support is arranged on the transverse rod, a dial indicator is connected with the position below the fixing support, and two same V-shaped blocks for placing parts are arranged on the base plate below the dial indicator along the direction of the transverse rod. The base plate and the stand columns are fixed through a bolt, the stand columns and the transverse rod are fixed through a bolt, the transverse rod and the fixing support are fixed through a bolt, and the fixing support and the dial indicator are fixed through a bolt. Wear-resisting hard blocks are arranged on positions of the V-shaped blocks contacted with the parts. The inspection device is convenient, rapid and efficient in detection process, greatly improves detection efficiency, greatly reduces labor intensity and operation difficulty of workers and simultaneously is high in detection accuracy.

The invention discloses a method for visually detecting the concentration of hydrogen peroxide by using carbon quantum dots. By virtue of a linear relationship between the concentration of the hydrogen peroxide in a mixed solution and the absorbance of the mixed solution, the concentration of the hydrogen peroxide is detected; the mixed solution contains TMB, the carbon quantum dots, a phosphoricacid and citric acid buffer and a phosphate buffer. By the method, when the carbon quantum dots are used for visually detecting the concentration of the hydrogen peroxide, the detection process is simple and convenient, the sensitivity is high, the detection limit is low, and rapid visual detection of the hydrogen peroxide in an actual sample can be achieved; the detection method has low detectioncost, a great application value and a broad market prospect; in addition, a water-soluble carbon quantum dot solution prepared on the basis of porcine blood is good in stability, simple in preparation method, convenient to operate, low in cost, low in toxicity, environmentally friendly and pollution-free; the obtained quantum dots have good water solubility and can be directly used for detectingthe concentration of the hydrogen peroxide, so that the detection complexity and the detection cost are reduced.

The invention relates to a multi-index parallel-detection protein chip detection kit based on a nitrocellulose membrane and a near-infrared fluorescent molecular marker. The multi-index parallel-detection protein chip detection kit comprises a micro-array protein chip, wherein the micro-array protein chip is formed by adopting the nitrocellulose membrane as a solid substrate and fixing different first proteins in different areas on the solid substrate; each first protein can be specifically combined with a disease marker in the body to form an immune complex, a second protein which can be specifically combined with the immune complex is marked by utilizing the near-infrared fluorescein molecules, and the second protein and the immune complex form a marker immune complex. An infrared fluorescent signal is a static signal and is not time-varied, and the signal and target protein concentration are in a proportional relation, so that the infrared fluorescent signal can be used for precise quantization; moreover, simplicity in operation is achieved, and the automation of the system can be realized. The invention also discloses a preparation method and a detection method of the protein chip detection kit.