The invention discloses a device and method for detecting holes of disposable gloves. The device for detecting holes of disposable gloves comprises a support, a plurality of detecting heads, a main control board and an air supplying 
system. Each detecting head comprises a guide shaft, a sliding sleeve, a movable spindle 
nose and a 
pressure sensor, wherein the sliding sleeve is in sliding connection with the guide shaft, the movable spindle 
nose is detachably connected to the guide shaft, a first air duct is formed in the guide shaft, the movable spindle 
nose is provided with a second air duct, the first air duct is in seal communication with the second air duct, a seal ring is arranged on the inner wall of the lower end of the sliding sleeve, a second seal ring is arranged outside the movable spindle nose, the 
pressure sensor is connected with the first air duct, the air supplying 
system is connected with the first air duct through an air 
pipe, the guide shaft is fixed to the support, and the air supplying 
system and the 
pressure sensor are respectively in electric connection with the main control board. The detecting process of the disposable gloves is simplified through the device for detecting holes of disposable gloves, and detecting efficiency is improved.