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Method for visually detecting concentration of hydrogen peroxide by using carbon quantum dots

A technology of carbon quantum dots and hydrogen peroxide, applied in the field of medicinal chemistry, can solve the problems of complex detection process, high detection cost and low detection cost, and achieve the effects of good water solubility, low detection cost and high sensitivity

Inactive Publication Date: 2019-02-15
GUANGDONG UNIV OF TECH
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Problems solved by technology

[0005] In order to overcome the defects of complicated detection process and high detection cost described in the above-mentioned prior art, the present invention provides a method for visually detecting hydrogen peroxide concentration by using carbon quantum dots. The process is simple and convenient, and can realize rapid and visual detection of hydrogen peroxide concentration in actual samples, and the reagents are cheap and the detection cost is low, which has great application value and broad market prospects

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  • Method for visually detecting concentration of hydrogen peroxide by using carbon quantum dots

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Embodiment 1

[0035] A method for visually detecting hydrogen peroxide concentration using carbon quantum dots, the specific steps are as follows

[0036] Step 1. Prepare a water-soluble carbon quantum dot solution based on pig blood, specifically:

[0037] Squeeze the porcine blood clot on filter paper to squeeze out water, then transfer the squeezed blood clot to an oven, dry it at 40-60°C, and then grind it into powder on a mortar. Dissolve pig blood powder in water at a mass ratio of 1% and transfer it to a reaction kettle, then fully react at 180 °C for 12 hours, cool at room temperature, and filter the reaction solution with a 0.45 μm microporous membrane to remove insoluble impurities to obtain to the water-soluble carbon quantum dot solution. The concentration of the water-soluble carbon quantum dot solution was 10 mg / mL.

[0038] Step 2, establish the linear relationship between the absorbance intensity of the mixed solution and the concentration of hydrogen peroxide, specificall...

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Abstract

The invention discloses a method for visually detecting the concentration of hydrogen peroxide by using carbon quantum dots. By virtue of a linear relationship between the concentration of the hydrogen peroxide in a mixed solution and the absorbance of the mixed solution, the concentration of the hydrogen peroxide is detected; the mixed solution contains TMB, the carbon quantum dots, a phosphoricacid and citric acid buffer and a phosphate buffer. By the method, when the carbon quantum dots are used for visually detecting the concentration of the hydrogen peroxide, the detection process is simple and convenient, the sensitivity is high, the detection limit is low, and rapid visual detection of the hydrogen peroxide in an actual sample can be achieved; the detection method has low detectioncost, a great application value and a broad market prospect; in addition, a water-soluble carbon quantum dot solution prepared on the basis of porcine blood is good in stability, simple in preparation method, convenient to operate, low in cost, low in toxicity, environmentally friendly and pollution-free; the obtained quantum dots have good water solubility and can be directly used for detectingthe concentration of the hydrogen peroxide, so that the detection complexity and the detection cost are reduced.

Description

technical field [0001] The invention relates to the technical field of medicinal chemistry, and more specifically, relates to a method for visually detecting hydrogen peroxide concentration by using carbon quantum dots. Background technique [0002] Hydrogen peroxide is commonly known as hydrogen peroxide, and its chemical formula is H 2 o 2 , organisms will produce H in the process of respiratory metabolism 2 o 2 . Hydrogen peroxide group, commonly known as "free radical", is generated by "catalase" catalyzing protein groups in organisms. This substance has relatively high oxidative properties and will damage normal biological macromolecules in cells such as proteins and DNA. Catalase is an enzyme that catalyzes the decomposition of hydrogen peroxide into oxygen and water. It exists in the peroxide body of cells and can convert H 2 o 2 catalytic decomposition into H 2 O and O 2 , harmless to the human body. Catalase is present in various tissues of all known anima...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 赵肃清杨慧怡何绮怡黎相广贺攀
Owner GUANGDONG UNIV OF TECH
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