Reagent for detecting SNP sites of P53 genes and application of reagent
A reagent and site technology, applied in the field of biomedicine, can solve the problems of less than 50% total effective rate, high detection cost, and high requirements for equipment and equipment, and achieves the effect of being beneficial to clinical promotion, simple detection process, and low equipment requirements.
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[0022] specific implementation
[0023] The present invention will be further described below through specific examples. It should be noted that, unless otherwise specified, the examples of the present invention are only used to explain the present invention, and are not meant to limit the protection scope of the present invention.
[0024] 1. Research objects: 87 patients with advanced non-small cell lung cancer. The selected patients (≥18 years old) were confirmed as advanced non-small cell lung cancer by histology or cytology, and all patients provided informed consent.
[0025] 2. Medication method: Gefitinib 250mg, once a day, orally. The medication cycle is 1 year.
[0026] 3. Selection of SNP sites of P53 gene: Search the P53 gene in the SNP public database (http: / / hapmap:ncbi.nlm.nih.gov / ) provided by the International Human Genome Hapmap Project, and first select the known Han nationality The SNP sites of the information in the population, and then use the tag SNPs...
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