Method for detecting concentration of horseradish peroxidase by utilizing water-soluble InP/ZnS QDs probe
A horseradish peroxidase and probe detection technology, which is applied in measuring devices, fluorescence/phosphorescence, and material analysis through optical means, can solve problems such as limited applications, achieve low detection limits, simple and convenient detection processes, and high sensitivity high effect
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[0026] Example 1
[0027] 1. Establish a linear relationship
[0028] Prepare the original solution of horseradish peroxidase of different concentrations, and add the same volume of water-soluble InP / ZnSQDs, namely water-soluble indium phosphide / zinc sulfide quantum dots, and then dilute to 3mL with phosphate buffer solution to prepare A series of equal-volume standard solutions of horseradish peroxidase with different concentrations. The concentration of horseradish peroxidase in several standard solutions is different, and the concentration of water-soluble InP / ZnSQDs is the same. Among them, the concentration of horseradish peroxidase in the standard solution The concentration is 0, 7×10 -9 mol / L, 1.5×10 -8 mol / L, 2.5×10 -8 mol / L、3×10 -8 mol / L、6×10 -8 mol / L, 1×10 -7 mol / L, 2×10 -7 mol / L and 4×10 -7 mol / L, its numbers are a, b, c, d, e, f, g, h, and i respectively, and the concentration of water-soluble InP / ZnSQDs in the standard solution is 23.04nM;
[0029] After the water-solub...
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[0033] Example 2
[0034] A method for detecting the concentration of horseradish peroxidase using water-soluble InP / ZnSQDs probe, including:
[0035] Step 1. Prepare different concentrations of water-soluble InP / ZnSQDs-containing horseradish peroxidase standard solutions, detect the fluorescence intensity of the standard solution, obtain the fluorescence spectrum of the standard solution, and establish that the horseradish peroxidase concentration is zero The linear relationship between the difference between the fluorescence intensity of the standard solution and the fluorescence intensity of all standard solutions and the concentration of horseradish peroxidase, such as figure 2 Shown
[0036] Step 2: Prepare a sample solution of horseradish peroxidase containing water-soluble InP / ZnSQDs, detect the fluorescence intensity of the sample solution, and determine the concentration of horseradish peroxidase in the sample solution through the linear relationship.
[0037] In the method...
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