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Method for detecting concentration of horseradish peroxidase by utilizing water-soluble InP/ZnS QDs probe

A horseradish peroxidase and probe detection technology, which is applied in measuring devices, fluorescence/phosphorescence, and material analysis through optical means, can solve problems such as limited applications, achieve low detection limits, simple and convenient detection processes, and high sensitivity high effect

Active Publication Date: 2016-07-20
铜陵华兴精细化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, traditional IIB-VI quantum dots such as CdTe, CdSe, etc., although their technical development is relatively mature, but the inherent shortcomings of Cd, a toxic element, will greatly limit their future applications.

Method used

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  • Method for detecting concentration of horseradish peroxidase by utilizing water-soluble InP/ZnS QDs probe
  • Method for detecting concentration of horseradish peroxidase by utilizing water-soluble InP/ZnS QDs probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Establish a linear relationship

[0028] Prepare different concentrations of horseradish peroxidase stock solutions, and add the same volume of water-soluble InP / ZnSQDs, that is, water-soluble indium phosphide / zinc sulfide quantum dots, and then dilute to 3mL with phosphate buffer solution to prepare A series of equal-volume standard solutions of horseradish peroxidase with different concentrations. The concentration of horseradish peroxidase in several standard solutions is different, and the concentration of water-soluble InP / ZnSQDs is the same. Wherein, the concentration of horseradish peroxidase in the standard solution Concentrations are 0, 7×10 -9 mol / L, 1.5×10 -8 mol / L, 2.5×10 -8 mol / L, 3×10 -8 mol / L, 6×10 -8 mol / L, 1×10 -7 mol / L, 2×10 -7 mol / L and 4×10 -7 mol / L, the numbers are a, b, c, d, e, f, g, h and i respectively, and the concentration of water-soluble InP / ZnSQDs in the standard solution is 23.04nM;

[0029] After the water-soluble InP / ZnSQDs pr...

Embodiment 2

[0034] A method utilizing water-soluble InP / ZnSQDs probes to detect horseradish peroxidase concentration, comprising:

[0035] Step 1, prepare the standard solutions of the horseradish peroxidase containing water-soluble InP / ZnSQDs of different concentrations, detect the fluorescence intensity of the standard solution, obtain the fluorescence spectrum of the standard solution, and establish the horseradish peroxidase concentration as zero The linear relationship between the fluorescence intensity of the standard solution and the difference of the fluorescence intensity of all standard solutions and the concentration of horseradish peroxidase, such as figure 2 shown;

[0036] Step 2: Prepare a sample solution of horseradish peroxidase containing water-soluble InP / ZnSQDs, detect the fluorescence intensity of the sample solution, and determine the concentration of horseradish peroxidase in the sample solution through the linear relationship.

[0037] In the described method tha...

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Abstract

The invention discloses a method for detecting concentration of horseradish peroxidase by utilizing a water-soluble InP / ZnS QDs probe. The method comprises the following steps: step 1, preparing horseradish peroxidase standard solutions which have different concentration and contain water-soluble InP / ZnS QDs; detecting the fluorescence intensity of the standard solutions to obtain fluorescence spectrograms of the standard solutions; establishing a linear relation of a difference value between the fluorescence intensity of the standard solution with the concentration of the horseradish peroxidase of zero and the fluorescence intensity of all the standard solutions and the concentration of the horseradish peroxidase; step 2, preparing a horseradish peroxidase sample solution containing the water-soluble InP / ZnS QDs and detecting the fluorescence intensity of the standard solution; and determining the concentration of the horseradish peroxidase in the sample solution through the linear solution. By taking the InP / ZnS QDs as the probe and utilizing the property of quenching the fluorescence of the water-soluble InP / ZnS QDs of the horseradish peroxidase, the concentration of the horseradish peroxidase is detected; and a detection process is simple and convenient, high in sensitivity and low in detection limit, and online and in-situ rapid and sensitive detection of the concentration of the horseradish peroxidase can be realized.

Description

technical field [0001] The invention relates to a method for detecting the concentration of horseradish peroxidase, in particular to a method for detecting the concentration of horseradish peroxidase by using a water-soluble InP / ZnSQDs probe. Background technique [0002] Horseradish peroxidase is a glycoprotein, the origin of its name is because of the high content of the enzyme in horseradish, it is currently the most widely used labeling enzyme, used to label antibodies or other proteins, both It can be used for positioning detection and also for quantitative determination. Horseradish peroxidase is widely used in the fields of industry, agriculture, medicine, and environmental protection. It can be used to prepare biochemical diagnostic kits for blood sugar, triglycerides, and cholesterol, as well as enzyme-labeled antibody performance in ELISA assays. [0003] In recent years, with the continuous advancement of quantum dot synthesis technology and the deepening of the ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 黄珊肖琦王鲁敏黄初升苏炜崔建国
Owner 铜陵华兴精细化工有限公司
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